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Protein

tRNA pseudouridine synthase B

Gene

truB

Organism
Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.UniRule annotation

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei46 – 461NucleophileUniRule annotation

GO - Molecular functioni

  1. pseudouridine synthase activity Source: UniProtKB-HAMAP
  2. RNA binding Source: InterPro

GO - Biological processi

  1. RNA processing Source: InterPro
  2. tRNA pseudouridine synthesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciNMUL323848:GKEC-1896-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase BUniRule annotation (EC:5.4.99.25UniRule annotation)
Alternative name(s):
tRNA pseudouridine(55) synthaseUniRule annotation
Short name:
Psi55 synthaseUniRule annotation
tRNA pseudouridylate synthaseUniRule annotation
tRNA-uridine isomeraseUniRule annotation
Gene namesi
Name:truBUniRule annotation
Ordered Locus Names:Nmul_A1862
OrganismiNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849)
Taxonomic identifieri323848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira
ProteomesiUP000002718 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313tRNA pseudouridine synthase BPRO_0000229365Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi323848.Nmul_A1862.

Structurei

3D structure databases

ProteinModelPortaliQ2Y7W4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0130.
HOGENOMiHOG000231223.
KOiK03177.
OMAiYELQFIR.
OrthoDBiEOG6358D3.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01080. TruB_bact.
InterProiIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR015240. tRNA_sdUridine_synth_fam1_C.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF09157. TruB-C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2Y7W4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPGSKRHIS GVLLLDKASG LSSNQALQTA KRIFSAHKAG HTGTLDPMAT
60 70 80 90 100
GLLPICFGEA TKFSSALLGA DKTYEAVLRL GYMSTTGDAE GEISIAAGME
110 120 130 140 150
SQYVDLTREK IEAVRKSFIG VITQVPPMYS AIKHRGKPMY TFARAGVEIE
160 170 180 190 200
RQPRAITIHD LSIEAYQGNE MRIRVTCGSG TYIRTLAEDL GHALGCGGAY
210 220 230 240 250
LTALRRSALG GFDLPQAYTL TGLEAMPPSQ RDSCLLPADS LLRSLPPVVV
260 270 280 290 300
DSAAALSLLQ GRAIPGTHPA GESLLPGRQV RLYDKAQRFL GLGEISTEGY
310
ISPKRLIRFE QSL
Length:313
Mass (Da):33,564
Last modified:December 20, 2005 - v1
Checksum:iA3FD1CF30B6604ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA. Translation: ABB75157.1.
RefSeqiWP_011381177.1. NC_007614.1.
YP_412549.1. NC_007614.1.

Genome annotation databases

EnsemblBacteriaiABB75157; ABB75157; Nmul_A1862.
KEGGinmu:Nmul_A1862.
PATRICi22727236. VBINitMul110821_2177.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA. Translation: ABB75157.1.
RefSeqiWP_011381177.1. NC_007614.1.
YP_412549.1. NC_007614.1.

3D structure databases

ProteinModelPortaliQ2Y7W4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323848.Nmul_A1862.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB75157; ABB75157; Nmul_A1862.
KEGGinmu:Nmul_A1862.
PATRICi22727236. VBINitMul110821_2177.

Phylogenomic databases

eggNOGiCOG0130.
HOGENOMiHOG000231223.
KOiK03177.
OMAiYELQFIR.
OrthoDBiEOG6358D3.

Enzyme and pathway databases

BioCyciNMUL323848:GKEC-1896-MONOMER.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01080. TruB_bact.
InterProiIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR015240. tRNA_sdUridine_synth_fam1_C.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF09157. TruB-C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC 25196."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25196 / NCIMB 11849.

Entry informationi

Entry nameiTRUB_NITMU
AccessioniPrimary (citable) accession number: Q2Y7W4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 20, 2005
Last modified: April 1, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.