Q2Y742 (DUT_NITMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	Nmul_A2136

Organism

Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849) [Complete proteome] [HAMAP]

Taxonomic identifier

323848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosospira

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity. HAMAP MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 149	149	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116		PRO_0000231416
Regions
	Region	68 – 70	3	Substrate binding By similarity
	Region	85 – 87	3	Substrate binding By similarity
Sites
	Binding site	81	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2Y742 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: C8C680569B825AA3	FASTA	149	15,973
	10 20 30 40 50 60 MKKIDIKILD SRLKDQLPAY ATSGSAGLDL RACIEHVMTI QPGEAHLIPT GIAIHLSDPG 70 80 90 100 110 120 LAALVLPRSG LGHKHGIVMG NLVGLIDSDY QGQIFVSCWN RGQAPFLLNP LERIAQLVVV 130 140 PVVQVGFKVV DDFEQSERGA NGFGSTGKH

References

[1]

"Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC 25196." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25196 / NCIMB 11849.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000103 Genomic DNA. Translation: ABB75429.1.
RefSeq	YP_412821.1. NC_007614.1.
3D structure databases
ProteinModelPortal	Q2Y742.
SMR	Q2Y742. Positions 1-136.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2Y742.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3784762.
GenomeReviews	Gene locus Nmul_A2136 in contig CP000103_GR.
KEGG	nmu:Nmul_A2136.
PATRIC	22727872. VBINitMul110821_2491.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HBG436079.
OMA	LDLRACI.
PhylomeDB	Q2Y742.
ProtClustDB	PRK00601.
Enzyme and pathway databases
BioCyc	NMUL323848:NMUL_A2136-MONOMER.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
KO	K01520.
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_NITMU

Accession

Primary (citable) accession number: Q2Y742

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program