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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100ATPUniRule annotation1
Active sitei311Tele-AMP-histidine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi285 – 289ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNMUL323848:G1GTI-2242-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:Nmul_A2158
OrganismiNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)
Taxonomic identifieri323848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira
Proteomesi
  • UP000002718 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002647971 – 353RNA 3'-terminal phosphate cyclaseAdd BLAST353

Interactioni

Protein-protein interaction databases

STRINGi323848.Nmul_A2158

Structurei

3D structure databases

ProteinModelPortaliQ2Y720
SMRiQ2Y720
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKX Bacteria
COG0430 LUCA
HOGENOMiHOG000015264
KOiK01974
OMAiQCLVYPL
OrthoDBiPOG091H0UB7

Family and domain databases

Gene3Di3.30.360.20, 1 hit
3.65.10.20, 2 hits
HAMAPiMF_00200 RTC, 1 hit
InterProiView protein in InterPro
IPR013791 RNA3'-term_phos_cycl_insert
IPR023797 RNA3'_phos_cyclase_dom
IPR037136 RNA3'_phos_cyclase_dom_sf
IPR000228 RNA3'_term_phos_cyc
IPR017770 RNA3'_term_phos_cyc_type_1
IPR020719 RNA3'_term_phos_cycl-like_CS
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
IPR036553 RPTC_insert
PANTHERiPTHR11096 PTHR11096, 1 hit
PTHR11096:SF0 PTHR11096:SF0, 1 hit
PfamiView protein in Pfam
PF01137 RTC, 1 hit
PF05189 RTC_insert, 1 hit
PIRSFiPIRSF005378 RNA3'_term_phos_cycl_euk, 1 hit
SUPFAMiSSF52913 SSF52913, 1 hit
SSF55205 SSF55205, 1 hit
TIGRFAMsiTIGR03399 RNA_3prim_cycl, 1 hit
PROSITEiView protein in PROSITE
PS01287 RTC, 1 hit

Sequencei

Sequence statusi: Complete.

Q2Y720-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEIDGSYGE GGGQLLRTSV ALAAITGQSV RVYNIRAKRS NPGLAPQHLT
60 70 80 90 100
AVKAVAALCR ARTEGMEVKS QEIIFRPGPL RGGEYDFPIG TAGSVTLVLQ
110 120 130 140 150
AALPVALACG EKVRMNISGG TDVRAAPPLD YFRYVLLPLV YSMGARAKIE
160 170 180 190 200
VLLRGYYPRG GGKVVVDVEP CLPLRPVLLN ASEGLEGITG FVHISNLPKH
210 220 230 240 250
IIHRMANGAL AELSTFPTPA VGLEVFGKDD AIGEGGAVLL TAHKEHSRLG
260 270 280 290 300
ASAVAERGVP AERLGAEAGR CLREEILSGA TLDIHAADQV LIYLALASGV
310 320 330 340 350
SCFLTRELSS HAATTIWLLE QFLPVRFQVT QEAHLIRVRA KPEFNGMSSF

LWR
Length:353
Mass (Da):37,750
Last modified:December 20, 2005 - v1
Checksum:i2E33AEFBC061A792
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA Translation: ABB75451.1
RefSeqiWP_011381460.1, NC_007614.1

Genome annotation databases

EnsemblBacteriaiABB75451; ABB75451; Nmul_A2158
KEGGinmu:Nmul_A2158

Similar proteinsi

Entry informationi

Entry nameiRTCA_NITMU
AccessioniPrimary (citable) accession number: Q2Y720
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health