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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100ATPUniRule annotation1
Active sitei311Tele-AMP-histidine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi285 – 289ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:Nmul_A2158
OrganismiNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)
Taxonomic identifieri323848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira
Proteomesi
  • UP000002718 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002647971 – 353RNA 3'-terminal phosphate cyclaseAdd BLAST353

Interactioni

Protein-protein interaction databases

STRINGi323848.Nmul_A2158.

Structurei

3D structure databases

ProteinModelPortaliQ2Y720.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKX. Bacteria.
COG0430. LUCA.
HOGENOMiHOG000015264.
KOiK01974.
OMAiNGCVDEF.
OrthoDBiPOG091H0UB7.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC. 1 hit.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PTHR11096:SF0. PTHR11096:SF0. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2Y720-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEIDGSYGE GGGQLLRTSV ALAAITGQSV RVYNIRAKRS NPGLAPQHLT
60 70 80 90 100
AVKAVAALCR ARTEGMEVKS QEIIFRPGPL RGGEYDFPIG TAGSVTLVLQ
110 120 130 140 150
AALPVALACG EKVRMNISGG TDVRAAPPLD YFRYVLLPLV YSMGARAKIE
160 170 180 190 200
VLLRGYYPRG GGKVVVDVEP CLPLRPVLLN ASEGLEGITG FVHISNLPKH
210 220 230 240 250
IIHRMANGAL AELSTFPTPA VGLEVFGKDD AIGEGGAVLL TAHKEHSRLG
260 270 280 290 300
ASAVAERGVP AERLGAEAGR CLREEILSGA TLDIHAADQV LIYLALASGV
310 320 330 340 350
SCFLTRELSS HAATTIWLLE QFLPVRFQVT QEAHLIRVRA KPEFNGMSSF

LWR
Length:353
Mass (Da):37,750
Last modified:December 20, 2005 - v1
Checksum:i2E33AEFBC061A792
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA. Translation: ABB75451.1.
RefSeqiWP_011381460.1. NC_007614.1.

Genome annotation databases

EnsemblBacteriaiABB75451; ABB75451; Nmul_A2158.
KEGGinmu:Nmul_A2158.
PATRICi22727924. VBINitMul110821_2517.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA. Translation: ABB75451.1.
RefSeqiWP_011381460.1. NC_007614.1.

3D structure databases

ProteinModelPortaliQ2Y720.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323848.Nmul_A2158.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB75451; ABB75451; Nmul_A2158.
KEGGinmu:Nmul_A2158.
PATRICi22727924. VBINitMul110821_2517.

Phylogenomic databases

eggNOGiENOG4105CKX. Bacteria.
COG0430. LUCA.
HOGENOMiHOG000015264.
KOiK01974.
OMAiNGCVDEF.
OrthoDBiPOG091H0UB7.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC. 1 hit.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PTHR11096:SF0. PTHR11096:SF0. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRTCA_NITMU
AccessioniPrimary (citable) accession number: Q2Y720
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.