Q2Y6S0 (TPMT_NITMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiopurine S-methyltransferase
EC=2.1.1.67

Alternative name(s):
Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	Nmul_A2259

Organism

Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849) [Complete proteome] [HAMAP]

Taxonomic identifier

323848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosospira

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity HAMAP MF_00812.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_1000047208
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	45	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2Y6S0 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: B4C1C58F32B88EF3	FASTA	220	25,563
	10 20 30 40 50 60 MKKEYWLERW RQEEIGFHQR EINPYLSRYW PELQVAPGKR VFVPLCGKSR DMIWLREQNL 70 80 90 100 110 120 SVLGVELSPL AVEAFFKENG YSPRHIIGEK FDQWDADGIH LLCGDFFDLK KDHLAQVDAV 130 140 150 160 170 180 YDRASLVALP PETRHAYTDH LLCILPPAIR ILLITFDYPQ AEMSGPPFAV STAEVEALYG 190 200 210 220 KRTDIRLLAK FDVLTENPRF QQRGISRLQE SIFLLMTRTA

References

[1]

"Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC 25196." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25196 / NCIMB 11849.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000103 Genomic DNA. Translation: ABB75551.1.
RefSeq	YP_412943.1. NC_007614.1.
3D structure databases
ProteinModelPortal	Q2Y6S0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2Y6S0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3785036.
GenomeReviews	Gene locus Nmul_A2259 in contig CP000103_GR.
KEGG	nmu:Nmul_A2259.
PATRIC	22728158. VBINitMul110821_2633.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0500.
HOGENOM	HBG444929.
OMA	QGDIFTL.
ProtClustDB	PRK13255.
Enzyme and pathway databases
BioCyc	NMUL323848:NMUL_A2259-MONOMER.
Family and domain databases
HAMAP	MF_00812. Thiopur_methtran. [Tree]
InterPro	IPR022474. Thiopur_S-MeTfrase_Se/Te_detox. IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
KO	K00569.
PANTHER	PTHR10259. PTHR10259. 1 hit.
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
TIGRFAMs	TIGR03840. TMPT_Se_Te. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_NITMU

Accession

Primary (citable) accession number: Q2Y6S0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program