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Q2Y641 (DDL_NITMU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Nmul_A2491
OrganismNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849) [Complete proteome] [HAMAP]
Taxonomic identifier323848 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira

Protein attributes

Sequence length306 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 306306D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341139

Regions

Domain106 – 301196ATP-grasp
Nucleotide binding132 – 18756ATP By similarity

Sites

Metal binding2551Magnesium or manganese 1 By similarity
Metal binding2681Magnesium or manganese 1 By similarity
Metal binding2681Magnesium or manganese 2 By similarity
Metal binding2701Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2Y641 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 4F0156B9B4989126

FASTA30632,910
        10         20         30         40         50         60 
MAGSHDFGKV AVLLGGRSAE REISLESGKA VLDALRSSGV DAHPFDPSEQ HMDALLQQGY 

        70         80         90        100        110        120 
TRAHIALHGR YGEDGTVQGA LELLGIPYTG SGVLASALAM DKWRTKLLWQ SAGINTPRHI 

       130        140        150        160        170        180 
LLDEQSDFDA VAKELGLPLI VKPSREGSTI GLSKVREAGE VAAAWHLAAR HDAMVLAEQF 

       190        200        210        220        230        240 
IEGTELTASI LGDVALPLVR IQVEGDLYDY QAKYLSDKTQ YFCPSGVSEE QECLIRKQAL 

       250        260        270        280        290        300 
QAHRLLGCEG WGRVDLILDK SGTPYFLEAN TSPGMTTHSL VPMAAKAAGI SFEELVLKIL 


GLAHVG 

« Hide

References

[1]"Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC 25196."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25196 / NCIMB 11849.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000103 Genomic DNA. Translation: ABB75780.1.
RefSeqYP_413172.1. NC_007614.1.

3D structure databases

ProteinModelPortalQ2Y641.
SMRQ2Y641. Positions 8-304.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323848.Nmul_A2491.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB75780; ABB75780; Nmul_A2491.
GeneID3786750.
KEGGnmu:Nmul_A2491.
PATRIC22728694. VBINitMul110821_2895.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycNMUL323848:GKEC-2537-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_NITMU
AccessionPrimary (citable) accession number: Q2Y641
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: December 20, 2005
Last modified: February 19, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways