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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi485MagnesiumUniRule annotation1
Metal bindingi491MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Nmul_A2553
OrganismiNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)
Taxonomic identifieri323848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira
Proteomesi
  • UP000002718 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003297391 – 701Polyribonucleotide nucleotidyltransferaseAdd BLAST701

Interactioni

Protein-protein interaction databases

STRINGi323848.Nmul_A2553.

Structurei

3D structure databases

ProteinModelPortaliQ2Y5X9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini552 – 611KHUniRule annotationAdd BLAST60
Domaini621 – 689S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2Y5X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIKKSVTYG SHQLTFETGE IARQAHAAIM VSMGDTVVLV TVVGAKSAKQ
60 70 80 90 100
GQDFFPLTVD YQERSYAAGR IPGSFFKREG RPSEKEILTS RLIDRPIRPL
110 120 130 140 150
FPENFYNEVQ VVATVLSSDN EVDADIPAML GASAALVLSG IPFNGPIGAA
160 170 180 190 200
RIGYINGEYV LNPDTSALKQ TQLNLVVAGT RQAVLMVESD AMELSEDVML
210 220 230 240 250
GAIVYGHQQM QVAIDAINEL ADEAGVTAWD WEPPARDPAL AVKIAELAEN
260 270 280 290 300
DLRAAFRQKQ KQVRSETIDN IWTRVFTELG VEDGEGPDAQ AVKEACFALE
310 320 330 340 350
SRIVRSQILD GEPRIDGRDT RTVRPITIRT GLLPRTHGSA LFTRGETQAL
360 370 380 390 400
VVATLGTGRD EQKIDALQGD YSERFMLHYN MPPYATGETG RVGTPKRREI
410 420 430 440 450
GHGRLAKRAL VAVLPSPEEF GYSLRVVSEI TESNGSSSMA SVCGGCLALM
460 470 480 490 500
DAGVPLKGHV AGIAMGLIKE GNRFAVLTDI LGDEDHLGDM DFKVAGTENG
510 520 530 540 550
ITALQMDIKI QGITKEILHA ALVQAREGRM HILAIMRQVL PARREDISEH
560 570 580 590 600
APRIIKIRIN PEKIRDVIGK GGAVIRALTE ETGTTIDITD DGTVMIACVN
610 620 630 640 650
AEGGELAKKR IEDITAEVEV GRVYDGTVLK LLDFGAIVSV LPGKDGLLHI
660 670 680 690 700
SQIANERVNN VGDHLKEGQV VRVKVLEADD KGRLRLSMKA VATDVADNAA

T
Length:701
Mass (Da):75,904
Last modified:December 20, 2005 - v1
Checksum:i0158E189FE1604C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA. Translation: ABB75842.1.
RefSeqiWP_011381841.1. NC_007614.1.

Genome annotation databases

EnsemblBacteriaiABB75842; ABB75842; Nmul_A2553.
KEGGinmu:Nmul_A2553.
PATRICi22728830. VBINitMul110821_2963.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA. Translation: ABB75842.1.
RefSeqiWP_011381841.1. NC_007614.1.

3D structure databases

ProteinModelPortaliQ2Y5X9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323848.Nmul_A2553.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB75842; ABB75842; Nmul_A2553.
KEGGinmu:Nmul_A2553.
PATRICi22728830. VBINitMul110821_2963.

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNP_NITMU
AccessioniPrimary (citable) accession number: Q2Y5X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.