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Q2Y5Q1

- GLND_NITMU

UniProt

Q2Y5Q1 - GLND_NITMU

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, Nmul_A2633
Organism
Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciNMUL323848:GKEC-2679-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:Nmul_A2633
OrganismiNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849)
Taxonomic identifieri323848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira
ProteomesiUP000002718: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 887887Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_0000231684Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi323848.Nmul_A2633.

Structurei

3D structure databases

ProteinModelPortaliQ2Y5Q1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini448 – 555108HD
Add
BLAST
Domaini687 – 77286ACT 1
Add
BLAST
Domaini796 – 87176ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 329329UridylyltransferaseUniRule annotation
Add
BLAST
Regioni330 – 686357Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2Y5Q1-1 [UniParc]FASTAAdd to Basket

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MKGLNAKPFS HEAVRQKLLS GRESLQRRYH QNRNGAALLR DHSRLVDGIL    50
RQVWHEMNMP GSTALLAVGG YGRRQLFPYS DIDLVVLLPD KGDAAEAMDN 100
ELGTRLEHWV GLLWDIGLDI GHSVRTVKEC GEEAANDITV QTSLLEARLL 150
GGNSDLFDRF LQVMETMLAP RKFFVDKQLE QQQRHKRYQD APYKLEPNIK 200
ESPGGLRDLQ NVLWISRAAG FGKTWSELAK KGFIIRREAR LIQRQQTVLQ 250
DLRIRLHYLG GRREDRLLFD YQNPLAEELG IAAKPPRRPG EMLMQRYYRA 300
ARSVTQVNTI LLLTLHAEIF PDEEAVTTII NERFQKRGDL LEICEEDIFE 350
RDPGAILESV LLLQQNPDLK GRSFATLRAM WRSAPLITAS FRREPRHCAM 400
FMEILRQPRG LTRELRLMNR YGILGRYIPA FGRIIGQMQH DLFHVYTVDE 450
HILMVVRNLR RFMAPEFAYE YPLCSRLINE FERPELLYLA GLFHDIAKGR 500
QGDHSLLGKV DARRFCERHR MPAEDTELVV WLVENHLHMS ATAQKKDIAD 550
AEVIADFAAS MRDERHLIAL YLLTVSDIRG TSPKVWNAWK GKLLEDLFHR 600
TRQYLCGETA LTDISLENRK NKVLQLLHPD DAAMRAYERF WSGLDSSYLM 650
MHDPREIAWH TQHLSQRMKS PAPIVKTRAA ETGAGVEVLV YTADQKDLFA 700
RICSFFDGID YNIVQAKIHT TREGYALDSF LVLDPFNVAN HDPREFQFIE 750
QELTQQLEQQ ALMATPVKGR LSRHLRHFPI TPQVSIEPDD SGAYYVLSIT 800
AGDQSGLLSR IAQVLVRFGL NVHSARINTL GERAEDTFLV TGSILSNSRS 850
VIQLEANLIK VLHTSPQPET PGKAPGKPSA GDRIIPR 887
Length:887
Mass (Da):101,510
Last modified:December 20, 2005 - v1
Checksum:iE65E0364AA37D84B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000103 Genomic DNA. Translation: ABB75920.1.
RefSeqiYP_413312.1. NC_007614.1.

Genome annotation databases

EnsemblBacteriaiABB75920; ABB75920; Nmul_A2633.
GeneIDi3786336.
KEGGinmu:Nmul_A2633.
PATRICi22729016. VBINitMul110821_3056.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000103 Genomic DNA. Translation: ABB75920.1 .
RefSeqi YP_413312.1. NC_007614.1.

3D structure databases

ProteinModelPortali Q2Y5Q1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 323848.Nmul_A2633.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB75920 ; ABB75920 ; Nmul_A2633 .
GeneIDi 3786336.
KEGGi nmu:Nmul_A2633.
PATRICi 22729016. VBINitMul110821_3056.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci NMUL323848:GKEC-2679-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC 25196."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25196 / NCIMB 11849.

Entry informationi

Entry nameiGLND_NITMU
AccessioniPrimary (citable) accession number: Q2Y5Q1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 20, 2005
Last modified: June 11, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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