Q2Y0W8 (S4A8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Electroneutral sodium bicarbonate exchanger 1

Alternative name(s):
Electroneutral Na(+)-driven Cl-HCO3 exchanger
Solute carrier family 4 member 8
k-NBC3

Gene names

Name:	SLC4A8
Synonyms:	KIAA0739, NBC, NBC3, NDCBE1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1093 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates electroneutral sodium- and carbonate-dependent chloride-HCO3- exchange with a Na+:HCO3- stoichiometry of 2:1. Plays a major role in pH regulation in neurons. May be involved in cell pH regulation by transporting HCO3- from blood to cell. Enhanced expression in severe acid stress could be important for cell survival by mediating the influx of HCO3- into the cells. Also mediates lithium-dependent HCO3- cotransport. May be regulated by osmolarity. Ref.1 Ref.7 UniProtKB Q8JZR6
Enzyme regulation	Activity is inhibited by 4,4'-Di-isothiocyanatostilbene-2,2'-disulfonic acid (DIDS - an inhibitor of several anion channels and transporters).
Subcellular location	Membrane; Multi-pass membrane protein Ref.9.
Tissue specificity	Expressed in the pyramidal cells of the hippocampus (at protein level). Highly expressed in all major regions of the brain, spinal column and in testis, and moderate levels in trachea, thyroid and medulla region of kidney. Low expression levels observed in pancreas and kidney cortex. Ref.1 Ref.7 Ref.9
Sequence similarities	Belongs to the anion exchanger (TC 2.A.31) family. [View classification]
Sequence caution	The sequence BAA34459.1 differs from that shown. Reason: Erroneous initiation. The sequence CAD38576.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Anion exchange Antiport Ion transport Sodium transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Transmembrane Transmembrane helix
Ligand	Sodium
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	bicarbonate transport Traceable author statement. Source: Reactome sodium ion transport Inferred from electronic annotation. Source: UniProtKB-KW transmembrane transport Traceable author statement. Source: Reactome
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Molecular_function	inorganic anion exchanger activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.2 (identifier: Q2Y0W8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.3 (identifier: Q2Y0W8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: MPAAGSNEPDGVLSYQ → MFNKNNSNKLRSTPRYRRGDPGYLNFTELGPLKPEQKDQWSQH
     1028-1093: EAEKMLEIGG...GNAKEKSLFN → VIVLAPTVYLGASNYRT

Note: No experimental confirmation available.

Isoform 3 Ref.1 (identifier: Q2Y0W8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1028-1093: EAEKMLEIGG...GNAKEKSLFN → VIVLAPTVYLGASNYRT

Note: May be due to an intron retention.

Isoform 4 Ref.2 (identifier: Q2Y0W8-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: Missing.
     1028-1093: EAEKMLEIGG...GNAKEKSLFN → VIVLAPTVYLGASNYRT

Note: May be due to an intron retention.

Isoform 5 Ref.2 (identifier: Q2Y0W8-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: Missing.

Isoform 6 Ref.5 Ref.6 (identifier: Q2Y0W8-6)

The sequence of this isoform differs from the canonical sequence as follows:
     671-691: ECQEMHGEFMGSACGHHGPYT → VSLGAARCPSIVTTGLGGTSK
     692-1093: Missing.

Isoform 7 Ref.4 (identifier: Q2Y0W8-7)

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: Missing.
     725-800: VRSMVSDFAV...LLCTILIFMD → MESCSVAWLE...RPPKMLGLQA
     801-1093: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1093	1093	Electroneutral sodium bicarbonate exchanger 1		PRO_0000328922
Regions
	Topological domain	1 – 478	478	Extracellular Potential
	Transmembrane	479 – 499	21	Helical; Potential
	Topological domain	500 – 523	24	Cytoplasmic Potential
	Transmembrane	524 – 544	21	Helical; Potential
	Topological domain	545 – 565	21	Extracellular Potential
	Transmembrane	566 – 586	21	Helical; Potential
	Topological domain	587 – 595	9	Cytoplasmic Potential
	Transmembrane	596 – 616	21	Helical; Potential
	Topological domain	617 – 687	71	Extracellular Potential
	Transmembrane	688 – 708	21	Helical; Potential
	Topological domain	709 – 731	23	Cytoplasmic Potential
	Transmembrane	732 – 752	21	Helical; Potential
	Topological domain	753 – 778	26	Extracellular Potential
	Transmembrane	779 – 799	21	Helical; Potential
	Topological domain	800 – 824	25	Cytoplasmic Potential
	Transmembrane	825 – 845	21	Helical; Potential
	Topological domain	846 – 881	36	Extracellular Potential
	Transmembrane	882 – 902	21	Helical; Potential
	Topological domain	903 – 904	2	Cytoplasmic Potential
	Transmembrane	905 – 925	21	Helical; Potential
	Topological domain	926 – 962	37	Extracellular Potential
	Transmembrane	963 – 983	21	Helical; Potential
	Topological domain	984 – 1093	110	Cytoplasmic Potential
	Coiled coil	1010 – 1036	27	Potential
Natural variations
	Alternative sequence	1 – 53	53	Missing in isoform 4, isoform 5 and isoform 7. Ref.2 Ref.4		VSP_052759
	Alternative sequence	1 – 16	16	MPAAG…VLSYQ → MFNKNNSNKLRSTPRYRRGD PGYLNFTELGPLKPEQKDQW SQH in isoform 2. Ref.3		VSP_052760
	Alternative sequence	671 – 691	21	ECQEM…HGPYT → VSLGAARCPSIVTTGLGGTS K in isoform 6. Ref.5 Ref.6		VSP_052761
	Alternative sequence	692 – 1093	402	Missing in isoform 6. Ref.5 Ref.6		VSP_052762
	Alternative sequence	725 – 800	76	VRSMV…LIFMD → MESCSVAWLECGGVILAHCN LRLLPSSWDYRHAPPRPANF CIFSGDGVSPCWPGWSQSLD LVICLPRPPKMLGLQA in isoform 7. Ref.4		VSP_052763
	Alternative sequence	801 – 1093	293	Missing in isoform 7. Ref.4		VSP_052764
	Alternative sequence	1028 – 1093	66	EAEKM…KSLFN → VIVLAPTVYLGASNYRT in isoform 2, isoform 3 and isoform 4. Ref.1 Ref.2 Ref.3		VSP_052765
	Natural variant	312	1	D → A. Corresponds to variant rs35966334 [ dbSNP | Ensembl ].		VAR_048351
	Natural variant	898	1	I → V. Corresponds to variant rs12318785 [ dbSNP | Ensembl ].		VAR_048352
Experimental info
	Sequence conflict	1008	1	M → T in AAC82380. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: DDF9D0BC3CBD1A06	FASTA	1,093	122,938
	10 20 30 40 50 60 MPAAGSNEPD GVLSYQRPDE EAVVDQGGTS TILNIHYEKE ELEGHRTLYV GVRMPLGRQS 70 80 90 100 110 120 HRHHRTHGQK HRRRGRGKGA SQGEEGLEAL AHDTPSQRVQ FILGTEEDEE HVPHELFTEL 130 140 150 160 170 180 DEICMKEGED AEWKETARWL KFEEDVEDGG ERWSKPYVAT LSLHSLFELR SCLINGTVLL 190 200 210 220 230 240 DMHANSIEEI SDLILDQQEL SSDLNDSMRV KVREALLKKH HHQNEKKRNN LIPIVRSFAE 250 260 270 280 290 300 VGKKQSDPHL MDKHGQTVSP QSVPTTNLEV KNGVNCEHSP VDLSKVDLHF MKKIPTGAEA 310 320 330 340 350 360 SNVLVGEVDI LDRPIVAFVR LSPAVLLSGL TEVPIPTRFL FILLGPVGKG QQYHEIGRSM 370 380 390 400 410 420 ATIMTDEIFH DVAYKAKERD DLLAGIDEFL DQVTVLPPGE WDPSIRIEPP KNVPSQEKRK 430 440 450 460 470 480 MPGVPNGNVC HIEQEPHGGH SGPELQRTGR LFGGLVLDIK RKAPWYWSDY RDALSLQCLA 490 500 510 520 530 540 SFLFLYCACM SPVITFGGLL GEATEGRISA IESLFGASMT GIAYSLFAGQ ALTILGSTGP 550 560 570 580 590 600 VLVFEKILFK FCKDYALSYL SLRACIGLWT AFLCIVLVAT DASSLVCYIT RFTEEAFASL 610 620 630 640 650 660 ICIIFIYEAI EKLIHLAETY PIHMHSQLDH LSLYYCRCTL PENPNNHTLQ YWKDHNIVTA 670 680 690 700 710 720 EVHWANLTVS ECQEMHGEFM GSACGHHGPY TPDVLFWSCI LFFTTFILSS TLKTFKTSRY 730 740 750 760 770 780 FPTRVRSMVS DFAVFLTIFT MVIIDFLIGV PSPKLQVPSV FKPTRDDRGW IINPIGPNPW 790 800 810 820 830 840 WTVIAAIIPA LLCTILIFMD QQITAVIINR KEHKLKKGCG YHLDLLMVAI MLGVCSIMGL 850 860 870 880 890 900 PWFVAATVLS ITHVNSLKLE SECSAPGEQP KFLGIREQRV TGLMIFVLMG CSVFMTAILK 910 920 930 940 950 960 FIPMPVLYGV FLYMGVSSLQ GIQFFDRLKL FGMPAKHQPD FIYLRHVPLR KVHLFTLIQL 970 980 990 1000 1010 1020 TCLVLLWVIK ASPAAIVFPM MVLALVFVRK VMDLCFSKRE LSWLDDLMPE SKKKKLDDAK 1030 1040 1050 1060 1070 1080 KKAKEEEEAE KMLEIGGDKF PLESRKLLSS PGKNISCRCD PSEINISDEM PKTTVWKALS 1090 MNSGNAKEKS LFN
	Isoform 2 [UniParc]. Checksum: 6CF28C557074A1EE	FASTA	1,071	120,987
	10 20 30 40 50 60 MFNKNNSNKL RSTPRYRRGD PGYLNFTELG PLKPEQKDQW SQHRPDEEAV VDQGGTSTIL 70 80 90 100 110 120 NIHYEKEELE GHRTLYVGVR MPLGRQSHRH HRTHGQKHRR RGRGKGASQG EEGLEALAHD 130 140 150 160 170 180 TPSQRVQFIL GTEEDEEHVP HELFTELDEI CMKEGEDAEW KETARWLKFE EDVEDGGERW 190 200 210 220 230 240 SKPYVATLSL HSLFELRSCL INGTVLLDMH ANSIEEISDL ILDQQELSSD LNDSMRVKVR 250 260 270 280 290 300 EALLKKHHHQ NEKKRNNLIP IVRSFAEVGK KQSDPHLMDK HGQTVSPQSV PTTNLEVKNG 310 320 330 340 350 360 VNCEHSPVDL SKVDLHFMKK IPTGAEASNV LVGEVDILDR PIVAFVRLSP AVLLSGLTEV 370 380 390 400 410 420 PIPTRFLFIL LGPVGKGQQY HEIGRSMATI MTDEIFHDVA YKAKERDDLL AGIDEFLDQV 430 440 450 460 470 480 TVLPPGEWDP SIRIEPPKNV PSQEKRKMPG VPNGNVCHIE QEPHGGHSGP ELQRTGRLFG 490 500 510 520 530 540 GLVLDIKRKA PWYWSDYRDA LSLQCLASFL FLYCACMSPV ITFGGLLGEA TEGRISAIES 550 560 570 580 590 600 LFGASMTGIA YSLFAGQALT ILGSTGPVLV FEKILFKFCK DYALSYLSLR ACIGLWTAFL 610 620 630 640 650 660 CIVLVATDAS SLVCYITRFT EEAFASLICI IFIYEAIEKL IHLAETYPIH MHSQLDHLSL 670 680 690 700 710 720 YYCRCTLPEN PNNHTLQYWK DHNIVTAEVH WANLTVSECQ EMHGEFMGSA CGHHGPYTPD 730 740 750 760 770 780 VLFWSCILFF TTFILSSTLK TFKTSRYFPT RVRSMVSDFA VFLTIFTMVI IDFLIGVPSP 790 800 810 820 830 840 KLQVPSVFKP TRDDRGWIIN PIGPNPWWTV IAAIIPALLC TILIFMDQQI TAVIINRKEH 850 860 870 880 890 900 KLKKGCGYHL DLLMVAIMLG VCSIMGLPWF VAATVLSITH VNSLKLESEC SAPGEQPKFL 910 920 930 940 950 960 GIREQRVTGL MIFVLMGCSV FMTAILKFIP MPVLYGVFLY MGVSSLQGIQ FFDRLKLFGM 970 980 990 1000 1010 1020 PAKHQPDFIY LRHVPLRKVH LFTLIQLTCL VLLWVIKASP AAIVFPMMVL ALVFVRKVMD 1030 1040 1050 1060 1070 LCFSKRELSW LDDLMPESKK KKLDDAKKKA KEEEVIVLAP TVYLGASNYR T
	Isoform 3 [UniParc]. Checksum: 083CF203D4D7FB55	FASTA	1,044	117,473
	10 20 30 40 50 60 MPAAGSNEPD GVLSYQRPDE EAVVDQGGTS TILNIHYEKE ELEGHRTLYV GVRMPLGRQS 70 80 90 100 110 120 HRHHRTHGQK HRRRGRGKGA SQGEEGLEAL AHDTPSQRVQ FILGTEEDEE HVPHELFTEL 130 140 150 160 170 180 DEICMKEGED AEWKETARWL KFEEDVEDGG ERWSKPYVAT LSLHSLFELR SCLINGTVLL 190 200 210 220 230 240 DMHANSIEEI SDLILDQQEL SSDLNDSMRV KVREALLKKH HHQNEKKRNN LIPIVRSFAE 250 260 270 280 290 300 VGKKQSDPHL MDKHGQTVSP QSVPTTNLEV KNGVNCEHSP VDLSKVDLHF MKKIPTGAEA 310 320 330 340 350 360 SNVLVGEVDI LDRPIVAFVR LSPAVLLSGL TEVPIPTRFL FILLGPVGKG QQYHEIGRSM 370 380 390 400 410 420 ATIMTDEIFH DVAYKAKERD DLLAGIDEFL DQVTVLPPGE WDPSIRIEPP KNVPSQEKRK 430 440 450 460 470 480 MPGVPNGNVC HIEQEPHGGH SGPELQRTGR LFGGLVLDIK RKAPWYWSDY RDALSLQCLA 490 500 510 520 530 540 SFLFLYCACM SPVITFGGLL GEATEGRISA IESLFGASMT GIAYSLFAGQ ALTILGSTGP 550 560 570 580 590 600 VLVFEKILFK FCKDYALSYL SLRACIGLWT AFLCIVLVAT DASSLVCYIT RFTEEAFASL 610 620 630 640 650 660 ICIIFIYEAI EKLIHLAETY PIHMHSQLDH LSLYYCRCTL PENPNNHTLQ YWKDHNIVTA 670 680 690 700 710 720 EVHWANLTVS ECQEMHGEFM GSACGHHGPY TPDVLFWSCI LFFTTFILSS TLKTFKTSRY 730 740 750 760 770 780 FPTRVRSMVS DFAVFLTIFT MVIIDFLIGV PSPKLQVPSV FKPTRDDRGW IINPIGPNPW 790 800 810 820 830 840 WTVIAAIIPA LLCTILIFMD QQITAVIINR KEHKLKKGCG YHLDLLMVAI MLGVCSIMGL 850 860 870 880 890 900 PWFVAATVLS ITHVNSLKLE SECSAPGEQP KFLGIREQRV TGLMIFVLMG CSVFMTAILK 910 920 930 940 950 960 FIPMPVLYGV FLYMGVSSLQ GIQFFDRLKL FGMPAKHQPD FIYLRHVPLR KVHLFTLIQL 970 980 990 1000 1010 1020 TCLVLLWVIK ASPAAIVFPM MVLALVFVRK VMDLCFSKRE LSWLDDLMPE SKKKKLDDAK 1030 1040 KKAKEEEVIV LAPTVYLGAS NYRT
	Isoform 4 [UniParc]. Checksum: 41DFC25D0B88F845	FASTA	991	111,661
	10 20 30 40 50 60 MPLGRQSHRH HRTHGQKHRR RGRGKGASQG EEGLEALAHD TPSQRVQFIL GTEEDEEHVP 70 80 90 100 110 120 HELFTELDEI CMKEGEDAEW KETARWLKFE EDVEDGGERW SKPYVATLSL HSLFELRSCL 130 140 150 160 170 180 INGTVLLDMH ANSIEEISDL ILDQQELSSD LNDSMRVKVR EALLKKHHHQ NEKKRNNLIP 190 200 210 220 230 240 IVRSFAEVGK KQSDPHLMDK HGQTVSPQSV PTTNLEVKNG VNCEHSPVDL SKVDLHFMKK 250 260 270 280 290 300 IPTGAEASNV LVGEVDILDR PIVAFVRLSP AVLLSGLTEV PIPTRFLFIL LGPVGKGQQY 310 320 330 340 350 360 HEIGRSMATI MTDEIFHDVA YKAKERDDLL AGIDEFLDQV TVLPPGEWDP SIRIEPPKNV 370 380 390 400 410 420 PSQEKRKMPG VPNGNVCHIE QEPHGGHSGP ELQRTGRLFG GLVLDIKRKA PWYWSDYRDA 430 440 450 460 470 480 LSLQCLASFL FLYCACMSPV ITFGGLLGEA TEGRISAIES LFGASMTGIA YSLFAGQALT 490 500 510 520 530 540 ILGSTGPVLV FEKILFKFCK DYALSYLSLR ACIGLWTAFL CIVLVATDAS SLVCYITRFT 550 560 570 580 590 600 EEAFASLICI IFIYEAIEKL IHLAETYPIH MHSQLDHLSL YYCRCTLPEN PNNHTLQYWK 610 620 630 640 650 660 DHNIVTAEVH WANLTVSECQ EMHGEFMGSA CGHHGPYTPD VLFWSCILFF TTFILSSTLK 670 680 690 700 710 720 TFKTSRYFPT RVRSMVSDFA VFLTIFTMVI IDFLIGVPSP KLQVPSVFKP TRDDRGWIIN 730 740 750 760 770 780 PIGPNPWWTV IAAIIPALLC TILIFMDQQI TAVIINRKEH KLKKGCGYHL DLLMVAIMLG 790 800 810 820 830 840 VCSIMGLPWF VAATVLSITH VNSLKLESEC SAPGEQPKFL GIREQRVTGL MIFVLMGCSV 850 860 870 880 890 900 FMTAILKFIP MPVLYGVFLY MGVSSLQGIQ FFDRLKLFGM PAKHQPDFIY LRHVPLRKVH 910 920 930 940 950 960 LFTLIQLTCL VLLWVIKASP AAIVFPMMVL ALVFVRKVMD LCFSKRELSW LDDLMPESKK 970 980 990 KKLDDAKKKA KEEEVIVLAP TVYLGASNYR T
	Isoform 5 [UniParc]. Checksum: A9CB5DD413DCDED7	FASTA	1,040	117,126
	10 20 30 40 50 60 MPLGRQSHRH HRTHGQKHRR RGRGKGASQG EEGLEALAHD TPSQRVQFIL GTEEDEEHVP 70 80 90 100 110 120 HELFTELDEI CMKEGEDAEW KETARWLKFE EDVEDGGERW SKPYVATLSL HSLFELRSCL 130 140 150 160 170 180 INGTVLLDMH ANSIEEISDL ILDQQELSSD LNDSMRVKVR EALLKKHHHQ NEKKRNNLIP 190 200 210 220 230 240 IVRSFAEVGK KQSDPHLMDK HGQTVSPQSV PTTNLEVKNG VNCEHSPVDL SKVDLHFMKK 250 260 270 280 290 300 IPTGAEASNV LVGEVDILDR PIVAFVRLSP AVLLSGLTEV PIPTRFLFIL LGPVGKGQQY 310 320 330 340 350 360 HEIGRSMATI MTDEIFHDVA YKAKERDDLL AGIDEFLDQV TVLPPGEWDP SIRIEPPKNV 370 380 390 400 410 420 PSQEKRKMPG VPNGNVCHIE QEPHGGHSGP ELQRTGRLFG GLVLDIKRKA PWYWSDYRDA 430 440 450 460 470 480 LSLQCLASFL FLYCACMSPV ITFGGLLGEA TEGRISAIES LFGASMTGIA YSLFAGQALT 490 500 510 520 530 540 ILGSTGPVLV FEKILFKFCK DYALSYLSLR ACIGLWTAFL CIVLVATDAS SLVCYITRFT 550 560 570 580 590 600 EEAFASLICI IFIYEAIEKL IHLAETYPIH MHSQLDHLSL YYCRCTLPEN PNNHTLQYWK 610 620 630 640 650 660 DHNIVTAEVH WANLTVSECQ EMHGEFMGSA CGHHGPYTPD VLFWSCILFF TTFILSSTLK 670 680 690 700 710 720 TFKTSRYFPT RVRSMVSDFA VFLTIFTMVI IDFLIGVPSP KLQVPSVFKP TRDDRGWIIN 730 740 750 760 770 780 PIGPNPWWTV IAAIIPALLC TILIFMDQQI TAVIINRKEH KLKKGCGYHL DLLMVAIMLG 790 800 810 820 830 840 VCSIMGLPWF VAATVLSITH VNSLKLESEC SAPGEQPKFL GIREQRVTGL MIFVLMGCSV 850 860 870 880 890 900 FMTAILKFIP MPVLYGVFLY MGVSSLQGIQ FFDRLKLFGM PAKHQPDFIY LRHVPLRKVH 910 920 930 940 950 960 LFTLIQLTCL VLLWVIKASP AAIVFPMMVL ALVFVRKVMD LCFSKRELSW LDDLMPESKK 970 980 990 1000 1010 1020 KKLDDAKKKA KEEEEAEKML EIGGDKFPLE SRKLLSSPGK NISCRCDPSE INISDEMPKT 1030 1040 TVWKALSMNS GNAKEKSLFN
	Isoform 6 [UniParc]. Checksum: EACAA9A36A17394B	FASTA	691	77,094
	10 20 30 40 50 60 MPAAGSNEPD GVLSYQRPDE EAVVDQGGTS TILNIHYEKE ELEGHRTLYV GVRMPLGRQS 70 80 90 100 110 120 HRHHRTHGQK HRRRGRGKGA SQGEEGLEAL AHDTPSQRVQ FILGTEEDEE HVPHELFTEL 130 140 150 160 170 180 DEICMKEGED AEWKETARWL KFEEDVEDGG ERWSKPYVAT LSLHSLFELR SCLINGTVLL 190 200 210 220 230 240 DMHANSIEEI SDLILDQQEL SSDLNDSMRV KVREALLKKH HHQNEKKRNN LIPIVRSFAE 250 260 270 280 290 300 VGKKQSDPHL MDKHGQTVSP QSVPTTNLEV KNGVNCEHSP VDLSKVDLHF MKKIPTGAEA 310 320 330 340 350 360 SNVLVGEVDI LDRPIVAFVR LSPAVLLSGL TEVPIPTRFL FILLGPVGKG QQYHEIGRSM 370 380 390 400 410 420 ATIMTDEIFH DVAYKAKERD DLLAGIDEFL DQVTVLPPGE WDPSIRIEPP KNVPSQEKRK 430 440 450 460 470 480 MPGVPNGNVC HIEQEPHGGH SGPELQRTGR LFGGLVLDIK RKAPWYWSDY RDALSLQCLA 490 500 510 520 530 540 SFLFLYCACM SPVITFGGLL GEATEGRISA IESLFGASMT GIAYSLFAGQ ALTILGSTGP 550 560 570 580 590 600 VLVFEKILFK FCKDYALSYL SLRACIGLWT AFLCIVLVAT DASSLVCYIT RFTEEAFASL 610 620 630 640 650 660 ICIIFIYEAI EKLIHLAETY PIHMHSQLDH LSLYYCRCTL PENPNNHTLQ YWKDHNIVTA 670 680 690 EVHWANLTVS VSLGAARCPS IVTTGLGGTS K
	Isoform 7 [UniParc]. Checksum: C50A2BC53EB6A41A	FASTA	747	83,897
	10 20 30 40 50 60 MPLGRQSHRH HRTHGQKHRR RGRGKGASQG EEGLEALAHD TPSQRVQFIL GTEEDEEHVP 70 80 90 100 110 120 HELFTELDEI CMKEGEDAEW KETARWLKFE EDVEDGGERW SKPYVATLSL HSLFELRSCL 130 140 150 160 170 180 INGTVLLDMH ANSIEEISDL ILDQQELSSD LNDSMRVKVR EALLKKHHHQ NEKKRNNLIP 190 200 210 220 230 240 IVRSFAEVGK KQSDPHLMDK HGQTVSPQSV PTTNLEVKNG VNCEHSPVDL SKVDLHFMKK 250 260 270 280 290 300 IPTGAEASNV LVGEVDILDR PIVAFVRLSP AVLLSGLTEV PIPTRFLFIL LGPVGKGQQY 310 320 330 340 350 360 HEIGRSMATI MTDEIFHDVA YKAKERDDLL AGIDEFLDQV TVLPPGEWDP SIRIEPPKNV 370 380 390 400 410 420 PSQEKRKMPG VPNGNVCHIE QEPHGGHSGP ELQRTGRLFG GLVLDIKRKA PWYWSDYRDA 430 440 450 460 470 480 LSLQCLASFL FLYCACMSPV ITFGGLLGEA TEGRISAIES LFGASMTGIA YSLFAGQALT 490 500 510 520 530 540 ILGSTGPVLV FEKILFKFCK DYALSYLSLR ACIGLWTAFL CIVLVATDAS SLVCYITRFT 550 560 570 580 590 600 EEAFASLICI IFIYEAIEKL IHLAETYPIH MHSQLDHLSL YYCRCTLPEN PNNHTLQYWK 610 620 630 640 650 660 DHNIVTAEVH WANLTVSECQ EMHGEFMGSA CGHHGPYTPD VLFWSCILFF TTFILSSTLK 670 680 690 700 710 720 TFKTSRYFPT RMESCSVAWL ECGGVILAHC NLRLLPSSWD YRHAPPRPAN FCIFSGDGVS 730 740 PCWPGWSQSL DLVICLPRPP KMLGLQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, characterization, and chromosomal mapping of a human electroneutral Na(+)-driven Cl-HCO3 exchanger." Grichtchenko I.I., Choi I., Zhong X., Bray-Ward P., Russell J.M., Boron W.F. J. Biol. Chem. 276:8358-8363(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, TISSUE SPECIFICITY, INHIBITION. Tissue: Frontal cortex.
[2]	"An electroneutral Na+-driven Cl-HCO3 exchanger (NDCBE1) with a novel COOH-terminus cloned from human brain." Bouyer P., Parker M.D., Boron W.F. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 4 AND 5). Tissue: Brain and Heart.
[3]	"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 7). Tissue: Amygdala.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 6). Tissue: Testis.
[7]	"Characterization of Na+/HCO-3 cotransporter isoform NBC-3." Amlal H., Burnham C.E., Soleimani M. Am. J. Physiol. 276:F903-F913(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-670 (ISOFORMS 1/3/6), FUNCTION, TISSUE SPECIFICITY, INHIBITION. Tissue: Testis carcinoma.
[8]	Erratum Amlal H., Burnham C.E., Soleimani M. Am. J. Physiol. 277:F477-F477(1999)
[9]	"Molecular expression of SLC4-derived Na+-dependent anion transporters in selected human tissues." Damkier H.H., Nielsen S., Praetorius J. Am. J. Physiol. 293:R2136-R2146(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF069512 mRNA. Translation: AAC82380.1. DQ063579 mRNA. Translation: AAY79176.1. DQ975204 mRNA. Translation: ABJ09587.1. DQ996537 mRNA. Translation: ABJ91576.1. DQ996398 mRNA. Translation: ABJ91577.1. AB018282 mRNA. Translation: BAA34459.1. Different initiation. AL831915 mRNA. Translation: CAD38576.2. Different initiation. CH471111 Genomic DNA. Translation: EAW58192.1. BC025994 mRNA. Translation: AAH25994.1. AF107099 mRNA. Translation: AAD52981.1.
IPI	IPI00097856. IPI00184876. IPI00737057. IPI00792986. IPI00794077. IPI00889606. IPI00889627.
RefSeq	NP_001035049.1. NM_001039960.2. NP_001245330.1. NM_001258401.2. NP_001245331.1. NM_001258402.1. NP_001245332.1. NM_001258403.1. NP_001254544.1. NM_001267615.1.
UniGene	Hs.4749.
3D structure databases
ProteinModelPortal	Q2Y0W8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q2Y0W8. 1 interaction.
STRING	9606.ENSP00000405812.
Protein family/group databases
TCDB	2.A.31.2.4. anion exchanger (AE) family.
PTM databases
PhosphoSite	Q2Y0W8.
Polymorphism databases
DMDM	121942008.
Proteomic databases
PaxDb	Q2Y0W8.
PRIDE	Q2Y0W8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000358657; ENSP00000351483; ENSG00000050438. ENST00000453097; ENSP00000405812; ENSG00000050438. ENST00000599597; ENSP00000472774; ENSG00000050438.
GeneID	9498.
KEGG	hsa:9498.
UCSC	uc001rym.3. human. uc001ryo.2. human. uc001ryq.4. human. uc001ryr.3. human. uc010snj.2. human.
Organism-specific databases
CTD	9498.
GeneCards	GC12P051807.
HGNC	HGNC:11034. SLC4A8.
HPA	HPA046665.
MIM	605024. gene.
neXtProt	NX_Q2Y0W8.
PharmGKB	PA35900.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG268067.
HOVERGEN	HBG004326.
KO	K13859.
OMA	VLNIHYE.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	Q2Y0W8.
Bgee	Q2Y0W8.
CleanEx	HS_SLC4A8.
Genevestigator	Q2Y0W8.
Family and domain databases
Gene3D	3.40.1100.10. 1 hit.
InterPro	IPR013769. Band3_cytoplasmic_dom. IPR011531. HCO3_transpt_C. IPR003020. HCO3_transpt_euk. IPR003024. Na/HCO3_transpt. IPR016152. PTrfase/Anion_transptr. [Graphical view]
PANTHER	PTHR11453. PTHR11453. 1 hit.
Pfam	PF07565. Band_3_cyto. 1 hit. PF00955. HCO3_cotransp. 1 hit. [Graphical view]
PRINTS	PR01231. HCO3TRNSPORT. PR01232. NAHCO3TRSPRT.
SUPFAM	SSF55804. PTrfase/Anion_transptr. 1 hit.
TIGRFAMs	TIGR00834. ae. 1 hit.
PROSITE	PS00219. ANION_EXCHANGER_1. False negative. PS00220. ANION_EXCHANGER_2. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SLC4A8. human.
GenomeRNAi	9498.
NextBio	35584.
SOURCE	Search...

Entry information

Entry name

S4A8_HUMAN

Accession

Primary (citable) accession number: Q2Y0W8
Secondary accession number(s): A0MMZ1 , O94843, O95233, Q004B4, Q8N3U2, Q8TC60, Q9UKX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.