Reviewed, UniProtKB/Swiss-Prot Q2XV99 (CP11A_MACFA)

Names and origin

Protein names

Recommended name:
    Cholesterol side-chain cleavage enzyme, mitochondrial
    EC=1.14.15.6
Alternative name(s):
    Cytochrome P450 11A1
    CYPXIA1
    P450(scc)
    Cholesterol desmolase

Gene names

Name:	CYP11A1
Synonyms:	CYP11A

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone By similarity.
Catalytic activity	Cholesterol + reduced adrenal ferredoxin + O2 = pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin + H2O.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; C21-steroid hormone metabolism.
Subcellular location	Mitochondrion membrane By similarity.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism Steroidogenesis
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	cholesterol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cholesterol monooxygenase (side-chain-cleaving) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Mitochondrion By similarity
	Chain	40 – 521	482	Cholesterol side-chain cleavage enzyme, mitochondrial		PRO_0000045764
Sites
	Metal binding	462	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2XV99-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 91B747E6AC00BA20	FASTA	521	60,242
	10 20 30 40 50 60 MLAKGLPPRS VLVKGCQTFL SAPKERLGHL RVPTSEGAGI STRSPRPFNE IPSPGDNGWL 70 80 90 100 110 120 NLYHFWRETG THKVHLHHVQ NFQKYDPIYR EKLGNVESVY VIDPEDVALL FKSEGPNPER 130 140 150 160 170 180 FLIPPWVAYH QYYQRPIGVL LKKSAAWKKD RVALNQEVMA PETTKNFLPL LDAVSRDFVS 190 200 210 220 230 240 VLHRRIKKAG SGNFSGDISD DLFRFAFESI TNVIFGERQG MLEEVVNPEG QRFIDAIYQM 250 260 270 280 290 300 FHTSVHMLNL PPDLFRLFRT KTWKDHVAPR DVIFSKADMY TENFHWELRQ KGNVHHDYRG 310 320 330 340 350 360 ILYRLLGDSK MSFEDIKANV TEMLAGGVDT TSMTLQWHLY EMARNLKVQD MLRAEVLAAR 370 380 390 400 410 420 RQAQGDMATI LQLVPLLKAS IKETLRLHPI SVTLQRYLVN DLVLRGYMIP AKTLVQVAIY 430 440 450 460 470 480 ALGREPTFFF DPENFDPTRW LSKDKNITYF RNLGFGWGVR QCLGRRIAEL EMTIFLINML 490 500 510 520 ENFRVEIQHL SDVGTTFNLI LMPEKPISFT FWPFNQEATQ E

References

[1]	Liu H., Larbie F., Luu-The V. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ228169 mRNA. Translation: ABB76810.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q2XV99.
Enzyme and pathway databases
BRENDA	1.14.15.6. 3438.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP11A_MACFA

Accession

Primary (citable) accession number: Q2XV99

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	December 20, 2005
Last modified:	October 13, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)