Reviewed, UniProtKB/Swiss-Prot Q2XU92 (ACBG2_MOUSE)

Names and origin

Protein names

Recommended name:
    Long-chain-fatty-acid--CoA ligase ACSBG2
    EC=6.2.1.3
Alternative name(s):
    Acyl-CoA synthetase bubblegum family member 2
    Bubblegum-related protein

Gene names

Name:	Acsbg2
Synonyms:	Bgr

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	667 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mediates activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Able to activate long-chain fatty acids. Also able to activate very long-chain fatty acids; however, the relevance of such activity is unclear in vivo. Has increased ability to activate oleic and linoleic acid. May play a role in spermatogenesis By similarity.
Catalytic activity	ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein.
Tissue specificity	Testis- and brainstem-specific. Expressed in pubertal and adult testis. Enriched in germ cells and Sertoli cells while present at a lower level in Leydig cells. Present in testicular Sertoli cells and large motoneurons in the medulla oblongata and cervical spinal cord (at protein level). Ref.1 Ref.2
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family. Bubblegum subfamily.

Ontologies

Keywords
Biological process	Differentiation Fatty acid metabolism Lipid metabolism Spermatogenesis
Cellular component	Cytoplasm Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Ligase
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW fatty acid beta-oxidation Ref.1 Inferred by curator. Source: MGI multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW spermatogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Ref.1 Inferred from direct assay. Source: MGI extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Ref.1 Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain-fatty-acid-CoA ligase activity Ref.1 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 667	667	Long-chain-fatty-acid--CoA ligase ACSBG2		PRO_0000315813
Regions
	Nucleotide binding	227 – 235	9	ATP By similarity
	Nucleotide binding	418 – 423	6	ATP By similarity
Sites
	Binding site	496	1	ATP By similarity
	Binding site	511	1	ATP By similarity
	Binding site	624	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2XU92-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 70E1D0EC8AC4726F	FASTA	667	74,319
	10 20 30 40 50 60 MTQEKKAEDL ERGTDATSAV PRLWSTHCDG EVLLRLSKHG PGHETPMTIP ELFQESAERF 70 80 90 100 110 120 SAYPALASKN GKKWDTLTFS QYYEMCRKAA KSLIKLGLQR FQCVGILGFN SVEWVVTALG 130 140 150 160 170 180 TILAGGLCVG IYATNSAEAC QYVIQQANVS ILIVENDQQL QKILLIPPDK METVKAIVQY 190 200 210 220 230 240 KLPLMESMAN LYSWNDFMEL GNDIPNIQLD RVILSQKANQ CAVILYTSGT TGTPKGVLLS 250 260 270 280 290 300 HDNITWTAGA MSQEMEINRV SGKQNTIVSY LPLSHIAAQL TDIWIPIKIG ALTFFAQPDA 310 320 330 340 350 360 LRGTLVYTLQ EVKPTLFMGV PRIWEKMQDT IKENVARSSR LRKKAFAWAK MLGLKVNTKR 370 380 390 400 410 420 MLGKRDIPMN YRMAKALVFA KVRTSLGLDN CHAFFSSASP LSQDVSEFFL SLDIPIGEIY 430 440 450 460 470 480 GMSECSGPHT VSNKSVYRVL SCGKVLSGCK NMLYNQNKEG VGEVCMWGRH VFMGYLNKEE 490 500 510 520 530 540 ATLEALDENG WLHSGDIGRL DSHDFLYITG RIKEILITAG GENVSPIPIE TLVKEKIPII 550 560 570 580 590 600 SHAMLVGDKA KFLCMLLTLK CETDRKSGEP LNKLSVEAKS FCQMLGSQAT TVSDILKSRD 610 620 630 640 650 660 QVVYTAIQYG IDIVNQQAMS DSHRIRKWII LEKDFSIQGG ELGPTSKLKR SVITQKYKAQ IDSMYLS

References

[1]	"The second member of the human and murine 'bubblegum' family is a testis-and brainstem-specific acyl-CoA synthetase." Pei Z., Jia Z., Watkins P.A. J. Biol. Chem. 281:6632-6641(2006) [PubMed: 16371355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: 129/SvEv.
[2]	"A novel mammalian bubblegum-related acyl-CoA synthetase restricted to testes and possibly involved in spermatogenesis." Fraisl P., Tanaka H., Forss-Petter S., Lassmann H., Nishimune Y., Berger J. Arch. Biochem. Biophys. 451:23-33(2006) [PubMed: 16762313] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ250679 mRNA. Translation: ABB54488.1.
IPI	IPI00342057.
RefSeq	NP_001034203.1.
UniGene	Mm.179421
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q2XU92.
Genome annotation databases
Ensembl	ENSMUST00000043062; ENSMUSP00000042352; ENSMUSG00000024207; Mus musculus. [Genome view]
GeneID	328845.
KEGG	mmu:328845.
UCSC	uc008ddi.1. mouse.
Organism-specific databases
CTD	328845.
MGI	MGI:3587728. Acsbg2.
Phylogenomic databases
HOGENOM	Q2XU92.
HOVERGEN	Q2XU92.
OMA	AAQMIDI.
Enzyme and pathway databases
BRENDA	6.2.1.3. 244.
Gene expression databases
ArrayExpress	Q2XU92.
Bgee	Q2XU92.
CleanEx	MM_ACSBG2.
Genevestigator	Q2XU92.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	398481.
SOURCE	Search...

Entry information

Entry name

ACBG2_MOUSE

Accession

Primary (citable) accession number: Q2XU92

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 20, 2005
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)