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Q2XSC4 (LABER_LAVAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Exo-alpha-bergamotene synthase
Short name=LaBERS
EC=4.2.3.81
Alternative name(s):
Trans-alpha-bergamotene synthase
OrganismLavandula angustifolia (Lavender)
Taxonomic identifier39329 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Lamiales › Lamiaceae › Nepetoideae › Lavanduleae › Lavandula

Protein attributes

Sequence length538 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes a mixture of sesquiterpenoids from (2E,6E)-farnesyl diphosphate. Catalyzes the formation of exo-alpha-bergamotene, as well as (E)-nerolidol, (Z)-alpha-bisabolene, (E)-beta-farnesene and beta-sesquiphellandrene. Also has activity towards geranyl diphosphate, but to a much lesser extent. Ref.1

Catalytic activity

(2E,6E)-farnesyl diphosphate = (-)-exo-alpha-bergamotene + diphosphate. Ref.1

Cofactor

Binds 3 magnesium or manganese ions per subunit By similarity.

Sequence similarities

Belongs to the terpene synthase family.

Biophysicochemical properties

Kinetic parameters:

kcat is 0.033 sec(-1) with (2E,6E)-farnesyl diphosphate as substrate. kcat is 0.00026 sec(-1) with geranyl diphosphate as substrate.

KM=4.7 µM for (2E,6E)-farnesyl diphosphate Ref.1

KM=3.3 µM for geranyl diphosphate

pH dependence:

Optimum pH is 8.0.

Ontologies

Keywords
   LigandMagnesium
Manganese
Metal-binding
   Molecular functionLyase
Gene Ontology (GO)
   Molecular_functionmagnesium ion binding

Inferred from electronic annotation. Source: InterPro

terpene synthase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 538538Exo-alpha-bergamotene synthase
PRO_0000418949

Regions

Motif291 – 2955DDXXD motif

Sites

Metal binding2911Magnesium or manganese 1 By similarity
Metal binding2911Magnesium or manganese 2 By similarity
Metal binding2951Magnesium or manganese 1 By similarity
Metal binding2951Magnesium or manganese 2 By similarity
Metal binding4351Magnesium or manganese 3 By similarity
Metal binding4391Magnesium or manganese 3 By similarity
Metal binding4431Magnesium or manganese 3 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2XSC4 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: E21A362A94398B89

FASTA53862,405
        10         20         30         40         50         60 
MEARRSGNFE SSIWDDDYIQ SLTSSYTGKM YVDKSEKLKI EVKMMMDEAT DELEQLELIN 

        70         80         90        100        110        120 
DLQRLGISYH FKDGIAKMLN NIYKSDSKYM EKDLHLTALK FRLLRQHGYR VPQDVFSSFM 

       130        140        150        160        170        180 
DDEGNFEAWV VEDVSVLVSL YEASHISVEG ESILDMAKDF SSHHLTEMVE QIGEACLAEQ 

       190        200        210        220        230        240 
VKRTLELPLH WRVGRLEARW FVQAYETRPN SNPTLVELAK LDFNMVQAKY QDELKRCSRW 

       250        260        270        280        290        300 
YEETGLPEKM SFARHRLAEC FLWSLGFIPD PHHGYSREIM TKIAVLITIT DDIYDIYGAL 

       310        320        330        340        350        360 
EELQEFTEAF ERWDINSLDL LPEYMQICFL AIFNSANELG YQILRDQGLN IIPNLKRSWA 

       370        380        390        400        410        420 
ELSRAYYLEA RWFHNGFVPT TDQYLNTAWI SISGPLLLSY GYLTTTNPIN NKELKSLEKH 

       430        440        450        460        470        480 
PSIIRWPSMV LRLADDLGTS SEEIKRGDVS KSIQCYMNET GCCEGDARHH VKSLIEVALK 

       490        500        510        520        530 
RMNDEILMEK PFKSFDTNAM NLARISLCFY QYGDGFGKPH SDTIKNLVSL IVLPFHMP

« Hide

References

[1]"Cloning and functional characterization of three terpene synthases from lavender (Lavandula angustifolia)."
Landmann C., Fink B., Festner M., Dregus M., Engel K.H., Schwab W.
Arch. Biochem. Biophys. 465:417-429(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		DQ263742 mRNA. Translation: ABB73046.1.

3D structure databases

ProteinModelPortalQ2XSC4.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-14818.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMSSF48239. Terp_cyc_toroid. 1 hit.
SSF48576. Terpenoid_synth. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLABER_LAVAN
AccessionPrimary (citable) accession number: Q2XSC4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: December 20, 2005
Last modified: May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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