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Q2XQU3

- BGAL2_ENTCL

UniProt

Q2XQU3 - BGAL2_ENTCL

Protein

Beta-galactosidase 2

Gene

lacZ

Organism
Enterobacter cloacae
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei104 – 1041SubstrateUniRule annotation
    Metal bindingi203 – 2031SodiumUniRule annotation
    Binding sitei203 – 2031SubstrateUniRule annotation
    Sitei393 – 3931Transition state stabilizerUniRule annotation
    Metal bindingi418 – 4181Magnesium 1UniRule annotation
    Metal bindingi420 – 4201Magnesium 1UniRule annotation
    Active sitei463 – 4631Proton donorUniRule annotation
    Metal bindingi463 – 4631Magnesium 1UniRule annotation
    Binding sitei463 – 4631SubstrateUniRule annotation
    Active sitei539 – 5391NucleophileUniRule annotation
    Metal bindingi599 – 5991Magnesium 2UniRule annotation
    Metal bindingi603 – 6031Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi606 – 6061SodiumUniRule annotation
    Binding sitei606 – 6061SubstrateUniRule annotation
    Binding sitei1004 – 10041SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase 2UniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Synonyms:bga
    OrganismiEnterobacter cloacae
    Taxonomic identifieri550 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10291029Beta-galactosidase 2PRO_0000366983Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ2XQU3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni539 – 5424Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2XQU3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPNTLSLTLS AILARRDWEN PGVTQWNRLE AHAPLHSWRL EQPALDDAAS     50
    ASRRSLNGVW RFNYFPAPEQ IPEAWVTEDC ADAVPMPVPS NWQMQGFDTP 100
    IYTNVTYPIP VNPPFVPQEN PTGCYSLTFD VDDAWLQSGQ TRIIFDGVNS 150
    AFHLWCNGRW MGYSQDSRLP AEFNLSTVLR PGENRLAVMV LRWCDGSYLE 200
    DQDMWRMSGI FRDVTLLHKP ETQIADYRVV TDLNAELDRA VLKADVALAG 250
    AGFADCEVVF TLWRKGEKCA SVSRRPGSAV VDERGSWDER LTVAIPIDRP 300
    ALWSAETPEL YRLTMALLGP QGEVLEVEAC DVGFRRVDIS NGLLKLNGKP 350
    LLIRGVNRLE HHPENGQVMD EATMRRDIEI MKQHNFNAVR CSHYPNHPLW 400
    YRLCDRYGLY VVDEANIETH GMVPMSRLAD DPRWLPAMSE RVTRMVQRDR 450
    NHPSIIIWSL GNESGHGANH DALYRWLKTT DPTRPVQYEG GGANTAATDI 500
    VCPMYARVDW DQPFPAVPKW SIKKWIGMPD ETRPLILCEY AHAMGNSFGG 550
    FAKYWQAFRS HPRLQGGFVW DWVDQALTKK DEKGNAFWAY GGDFGDTPND 600
    RQFCLNGLVF PDRTPHPALY EAQRAQQFFT FTLVSTSPLM IEVQSGYLFR 650
    PTDNEVLSWT VARDGKVLAS GEVTLAIAPE GVQRLEIALP ELKAGPGEIW 700
    LNVEVRQPRA TPWSPAGHRC AWEQWPLPAP LFIAPPASTG EPPVLTQNDR 750
    ILEVTHRQQR WQFDRASGYL TQWWRNGVET LLSPVTDNVS RAPLDNDIGV 800
    SEATRIDPNA WVERWKAAGM YDLTSRMLHC EAEQHAREVV VTTLNVLEHR 850
    GRALFLSRKI WRLDEQGVLH GDIQVDIASD IPKPARIGLS VHLAETPEKV 900
    DWLGLGPHEN YPDRKLAAQQ GRWTLPLADM HTPYIFPTEN GLRCDTRKLV 950
    LGAHQLNGAF HFSVGRYSQQ QLRETTHHHL LREEPGGWLN LDAFHMGVGG 1000
    DDSWSPSVSP EFILQTRQLR YTFSWQQNP 1029
    Length:1,029
    Mass (Da):116,816
    Last modified:December 20, 2005 - v1
    Checksum:iC96F1A32A29E2241
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ266449 Genomic DNA. Translation: ABB73039.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ266449 Genomic DNA. Translation: ABB73039.1 .

    3D structure databases

    ProteinModelPortali Q2XQU3.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Purification, characterization, and molecular cloning of a beta-galactosidase with transglycosylation activity from Enterobacter cloacae."
      Lu L.L., Xiao M.
      Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: B5.

    Entry informationi

    Entry nameiBGAL2_ENTCL
    AccessioniPrimary (citable) accession number: Q2XQU3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3