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Reviewed, UniProtKB/Swiss-Prot Q2XQU3 (BGAL2_ENTCL)

Last modified June 16, 2009. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Beta-galactosidase 2
      Short name=Beta-gal
    EC=3.2.1.23
Alternative name(s):
    Lactase
Gene names
Name: lacZ
Synonyms: bga
OrganismEnterobacter cloacae
Taxonomic identifier550 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter › Enterobacter cloacae complex

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Protein attributes

Sequence length1029 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity.

Binds 1 sodium ion per monomer By similarity.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10291029Beta-galactosidase 2 HAMAP MF_01687
PRO_0000366983

Regions

Region539 – 5424Substrate binding By similarity

Sites

Active site4631Proton donor By similarity
Active site5391Nucleophile By similarity
Metal binding2031Sodium By similarity
Metal binding4181Magnesium 1 By similarity
Metal binding4201Magnesium 1 By similarity
Metal binding4631Magnesium 1 By similarity
Metal binding5991Magnesium 2 By similarity
Metal binding6031Sodium; via carbonyl oxygen By similarity
Metal binding6061Sodium By similarity
Binding site1041Substrate By similarity
Binding site2031Substrate By similarity
Binding site4631Substrate By similarity
Binding site6061Substrate By similarity
Binding site10041Substrate By similarity
Site3931Transition state stabilizer By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q2XQU3-1 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: C96F1A32A29E2241

FASTA1,029116,816
        10         20         30         40         50         60 
MPNTLSLTLS AILARRDWEN PGVTQWNRLE AHAPLHSWRL EQPALDDAAS ASRRSLNGVW 

        70         80         90        100        110        120 
RFNYFPAPEQ IPEAWVTEDC ADAVPMPVPS NWQMQGFDTP IYTNVTYPIP VNPPFVPQEN 

       130        140        150        160        170        180 
PTGCYSLTFD VDDAWLQSGQ TRIIFDGVNS AFHLWCNGRW MGYSQDSRLP AEFNLSTVLR 

       190        200        210        220        230        240 
PGENRLAVMV LRWCDGSYLE DQDMWRMSGI FRDVTLLHKP ETQIADYRVV TDLNAELDRA 

       250        260        270        280        290        300 
VLKADVALAG AGFADCEVVF TLWRKGEKCA SVSRRPGSAV VDERGSWDER LTVAIPIDRP 

       310        320        330        340        350        360 
ALWSAETPEL YRLTMALLGP QGEVLEVEAC DVGFRRVDIS NGLLKLNGKP LLIRGVNRLE 

       370        380        390        400        410        420 
HHPENGQVMD EATMRRDIEI MKQHNFNAVR CSHYPNHPLW YRLCDRYGLY VVDEANIETH 

       430        440        450        460        470        480 
GMVPMSRLAD DPRWLPAMSE RVTRMVQRDR NHPSIIIWSL GNESGHGANH DALYRWLKTT 

       490        500        510        520        530        540 
DPTRPVQYEG GGANTAATDI VCPMYARVDW DQPFPAVPKW SIKKWIGMPD ETRPLILCEY 

       550        560        570        580        590        600 
AHAMGNSFGG FAKYWQAFRS HPRLQGGFVW DWVDQALTKK DEKGNAFWAY GGDFGDTPND 

       610        620        630        640        650        660 
RQFCLNGLVF PDRTPHPALY EAQRAQQFFT FTLVSTSPLM IEVQSGYLFR PTDNEVLSWT 

       670        680        690        700        710        720 
VARDGKVLAS GEVTLAIAPE GVQRLEIALP ELKAGPGEIW LNVEVRQPRA TPWSPAGHRC 

       730        740        750        760        770        780 
AWEQWPLPAP LFIAPPASTG EPPVLTQNDR ILEVTHRQQR WQFDRASGYL TQWWRNGVET 

       790        800        810        820        830        840 
LLSPVTDNVS RAPLDNDIGV SEATRIDPNA WVERWKAAGM YDLTSRMLHC EAEQHAREVV 

       850        860        870        880        890        900 
VTTLNVLEHR GRALFLSRKI WRLDEQGVLH GDIQVDIASD IPKPARIGLS VHLAETPEKV 

       910        920        930        940        950        960 
DWLGLGPHEN YPDRKLAAQQ GRWTLPLADM HTPYIFPTEN GLRCDTRKLV LGAHQLNGAF 

       970        980        990       1000       1010       1020 
HFSVGRYSQQ QLRETTHHHL LREEPGGWLN LDAFHMGVGG DDSWSPSVSP EFILQTRQLR 


YTFSWQQNP

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References

[1]"Purification, characterization, and molecular cloning of a beta-galactosidase with transglycosylation activity from Enterobacter cloacae."
Lu L.L., Xiao M.
Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: B5.

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Cross-references

Sequence databases

DQ266449 Genomic DNA. Translation: ABB73039.1.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Family and domain databases

HAMAPMF_01687.
[Tree]
InterProIPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006104. Glyco_hydro_2_carb-bd.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006103. Glyco_hydro_2_TIM.
IPR004199. Glyco_hydro_42_D5.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]
Gene3DG3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits.
G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameBGAL2_ENTCL
AccessionPrimary (citable) accession number: Q2XQU3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 20, 2005
Last modified: June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents