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Q2XQU3

- BGAL2_ENTCL

UniProt

Q2XQU3 - BGAL2_ENTCL

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Protein

Beta-galactosidase 2

Gene

lacZ

Organism
Enterobacter cloacae
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na(+)UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041SubstrateUniRule annotation
Metal bindingi203 – 2031SodiumUniRule annotation
Binding sitei203 – 2031SubstrateUniRule annotation
Sitei393 – 3931Transition state stabilizerUniRule annotation
Metal bindingi418 – 4181Magnesium 1UniRule annotation
Metal bindingi420 – 4201Magnesium 1UniRule annotation
Active sitei463 – 4631Proton donorUniRule annotation
Metal bindingi463 – 4631Magnesium 1UniRule annotation
Binding sitei463 – 4631SubstrateUniRule annotation
Active sitei539 – 5391NucleophileUniRule annotation
Metal bindingi599 – 5991Magnesium 2UniRule annotation
Metal bindingi603 – 6031Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi606 – 6061SodiumUniRule annotation
Binding sitei606 – 6061SubstrateUniRule annotation
Binding sitei1004 – 10041SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 2UniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Synonyms:bga
OrganismiEnterobacter cloacae
Taxonomic identifieri550 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10291029Beta-galactosidase 2PRO_0000366983Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ2XQU3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni539 – 5424Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2XQU3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPNTLSLTLS AILARRDWEN PGVTQWNRLE AHAPLHSWRL EQPALDDAAS
60 70 80 90 100
ASRRSLNGVW RFNYFPAPEQ IPEAWVTEDC ADAVPMPVPS NWQMQGFDTP
110 120 130 140 150
IYTNVTYPIP VNPPFVPQEN PTGCYSLTFD VDDAWLQSGQ TRIIFDGVNS
160 170 180 190 200
AFHLWCNGRW MGYSQDSRLP AEFNLSTVLR PGENRLAVMV LRWCDGSYLE
210 220 230 240 250
DQDMWRMSGI FRDVTLLHKP ETQIADYRVV TDLNAELDRA VLKADVALAG
260 270 280 290 300
AGFADCEVVF TLWRKGEKCA SVSRRPGSAV VDERGSWDER LTVAIPIDRP
310 320 330 340 350
ALWSAETPEL YRLTMALLGP QGEVLEVEAC DVGFRRVDIS NGLLKLNGKP
360 370 380 390 400
LLIRGVNRLE HHPENGQVMD EATMRRDIEI MKQHNFNAVR CSHYPNHPLW
410 420 430 440 450
YRLCDRYGLY VVDEANIETH GMVPMSRLAD DPRWLPAMSE RVTRMVQRDR
460 470 480 490 500
NHPSIIIWSL GNESGHGANH DALYRWLKTT DPTRPVQYEG GGANTAATDI
510 520 530 540 550
VCPMYARVDW DQPFPAVPKW SIKKWIGMPD ETRPLILCEY AHAMGNSFGG
560 570 580 590 600
FAKYWQAFRS HPRLQGGFVW DWVDQALTKK DEKGNAFWAY GGDFGDTPND
610 620 630 640 650
RQFCLNGLVF PDRTPHPALY EAQRAQQFFT FTLVSTSPLM IEVQSGYLFR
660 670 680 690 700
PTDNEVLSWT VARDGKVLAS GEVTLAIAPE GVQRLEIALP ELKAGPGEIW
710 720 730 740 750
LNVEVRQPRA TPWSPAGHRC AWEQWPLPAP LFIAPPASTG EPPVLTQNDR
760 770 780 790 800
ILEVTHRQQR WQFDRASGYL TQWWRNGVET LLSPVTDNVS RAPLDNDIGV
810 820 830 840 850
SEATRIDPNA WVERWKAAGM YDLTSRMLHC EAEQHAREVV VTTLNVLEHR
860 870 880 890 900
GRALFLSRKI WRLDEQGVLH GDIQVDIASD IPKPARIGLS VHLAETPEKV
910 920 930 940 950
DWLGLGPHEN YPDRKLAAQQ GRWTLPLADM HTPYIFPTEN GLRCDTRKLV
960 970 980 990 1000
LGAHQLNGAF HFSVGRYSQQ QLRETTHHHL LREEPGGWLN LDAFHMGVGG
1010 1020
DDSWSPSVSP EFILQTRQLR YTFSWQQNP
Length:1,029
Mass (Da):116,816
Last modified:December 20, 2005 - v1
Checksum:iC96F1A32A29E2241
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ266449 Genomic DNA. Translation: ABB73039.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ266449 Genomic DNA. Translation: ABB73039.1 .

3D structure databases

ProteinModelPortali Q2XQU3.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Purification, characterization, and molecular cloning of a beta-galactosidase with transglycosylation activity from Enterobacter cloacae."
    Lu L.L., Xiao M.
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: B5.

Entry informationi

Entry nameiBGAL2_ENTCL
AccessioniPrimary (citable) accession number: Q2XQU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 20, 2005
Last modified: November 26, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3