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Q2XQU3

- BGAL2_ENTCL

UniProt

Q2XQU3 - BGAL2_ENTCL

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Protein
Beta-galactosidase 2
Gene
lacZ, bga
Organism
Enterobacter cloacae
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer By similarity.UniRule annotation
Binds 1 sodium ion per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041Substrate By similarity
Metal bindingi203 – 2031Sodium By similarity
Binding sitei203 – 2031Substrate By similarity
Sitei393 – 3931Transition state stabilizer By similarity
Metal bindingi418 – 4181Magnesium 1 By similarity
Metal bindingi420 – 4201Magnesium 1 By similarity
Active sitei463 – 4631Proton donor By similarity
Metal bindingi463 – 4631Magnesium 1 By similarity
Binding sitei463 – 4631Substrate By similarity
Active sitei539 – 5391Nucleophile By similarity
Metal bindingi599 – 5991Magnesium 2 By similarity
Metal bindingi603 – 6031Sodium; via carbonyl oxygen By similarity
Metal bindingi606 – 6061Sodium By similarity
Binding sitei606 – 6061Substrate By similarity
Binding sitei1004 – 10041Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 2 (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Synonyms:bga
OrganismiEnterobacter cloacae
Taxonomic identifieri550 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10291029Beta-galactosidase 2UniRule annotation
PRO_0000366983Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ2XQU3.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni539 – 5424Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2XQU3-1 [UniParc]FASTAAdd to Basket

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MPNTLSLTLS AILARRDWEN PGVTQWNRLE AHAPLHSWRL EQPALDDAAS     50
ASRRSLNGVW RFNYFPAPEQ IPEAWVTEDC ADAVPMPVPS NWQMQGFDTP 100
IYTNVTYPIP VNPPFVPQEN PTGCYSLTFD VDDAWLQSGQ TRIIFDGVNS 150
AFHLWCNGRW MGYSQDSRLP AEFNLSTVLR PGENRLAVMV LRWCDGSYLE 200
DQDMWRMSGI FRDVTLLHKP ETQIADYRVV TDLNAELDRA VLKADVALAG 250
AGFADCEVVF TLWRKGEKCA SVSRRPGSAV VDERGSWDER LTVAIPIDRP 300
ALWSAETPEL YRLTMALLGP QGEVLEVEAC DVGFRRVDIS NGLLKLNGKP 350
LLIRGVNRLE HHPENGQVMD EATMRRDIEI MKQHNFNAVR CSHYPNHPLW 400
YRLCDRYGLY VVDEANIETH GMVPMSRLAD DPRWLPAMSE RVTRMVQRDR 450
NHPSIIIWSL GNESGHGANH DALYRWLKTT DPTRPVQYEG GGANTAATDI 500
VCPMYARVDW DQPFPAVPKW SIKKWIGMPD ETRPLILCEY AHAMGNSFGG 550
FAKYWQAFRS HPRLQGGFVW DWVDQALTKK DEKGNAFWAY GGDFGDTPND 600
RQFCLNGLVF PDRTPHPALY EAQRAQQFFT FTLVSTSPLM IEVQSGYLFR 650
PTDNEVLSWT VARDGKVLAS GEVTLAIAPE GVQRLEIALP ELKAGPGEIW 700
LNVEVRQPRA TPWSPAGHRC AWEQWPLPAP LFIAPPASTG EPPVLTQNDR 750
ILEVTHRQQR WQFDRASGYL TQWWRNGVET LLSPVTDNVS RAPLDNDIGV 800
SEATRIDPNA WVERWKAAGM YDLTSRMLHC EAEQHAREVV VTTLNVLEHR 850
GRALFLSRKI WRLDEQGVLH GDIQVDIASD IPKPARIGLS VHLAETPEKV 900
DWLGLGPHEN YPDRKLAAQQ GRWTLPLADM HTPYIFPTEN GLRCDTRKLV 950
LGAHQLNGAF HFSVGRYSQQ QLRETTHHHL LREEPGGWLN LDAFHMGVGG 1000
DDSWSPSVSP EFILQTRQLR YTFSWQQNP 1029
Length:1,029
Mass (Da):116,816
Last modified:December 20, 2005 - v1
Checksum:iC96F1A32A29E2241
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ266449 Genomic DNA. Translation: ABB73039.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ266449 Genomic DNA. Translation: ABB73039.1 .

3D structure databases

ProteinModelPortali Q2XQU3.
ModBasei Search...

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Purification, characterization, and molecular cloning of a beta-galactosidase with transglycosylation activity from Enterobacter cloacae."
    Lu L.L., Xiao M.
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: B5.

Entry informationi

Entry nameiBGAL2_ENTCL
AccessioniPrimary (citable) accession number: Q2XQU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 20, 2005
Last modified: February 19, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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