Q2XPU7 (LUPS_RICCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Lupeol synthase EC=5.4.99.41
Organism	Ricinus communis (Castor bean) [Complete proteome]
Taxonomic identifier	3988 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Malpighiales › Euphorbiaceae › Acalyphoideae › Acalypheae › Ricinus

Protein attributes

Sequence length	769 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Oxidosqualene cyclase involved in the biosynthesis of the cuticular lupeol of glaucous individuals, but not of the glossy phenotype. Ref.1
Catalytic activity	(3S)-2,3-epoxy-2,3-dihydrosqualene = lupeol. Ref.1
Tissue specificity	Expressed in stems and laves of glaucous individuals. Not expressed neither in stems or leaves of the glossy phenotype, nor in roots of both phenotypes. Ref.1
Developmental stage	Expressed between 6 and 50 days after emergence, with a peak at day 12. Ref.1
Miscellaneous	Lupeol forms probably the thread-shaped epicuticular wax crystals that hamper insect adhesion and can prevent herbivores from climbing vertical plant structures, a phenomenon known as the 'greasy pole syndrome' (Ref.1).
Sequence similarities	Belongs to the terpene cyclase/mutase family. Contains 4 PFTB repeats.

Ontologies

Keywords
Domain	Repeat
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cuticle development Inferred from direct assay Ref.1. Source: UniProtKB pentacyclic triterpenoid biosynthetic process Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	lupeol synthase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 769	769	Lupeol synthase		PRO_0000413996
Regions
	Repeat	148 – 189	42	PFTB 1
	Repeat	513 – 558	46	PFTB 2
	Repeat	590 – 630	41	PFTB 3
	Repeat	639 – 680	42	PFTB 4
	Compositional bias	94 – 97	4	Poly-Ala

Sequences

	Sequence		Length	Mass (Da)
	Q2XPU7 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 02BC53319F087E92	FASTA	769	88,321
	10 20 30 40 50 60 MWRIKIAEGG NNPYIYSTNN FQGRQIWVFD PNAGTPEEQA EVEEARQNFW KNRFQVKPNS 70 80 90 100 110 120 DLLWQLQFLR EKNFKQKIPK VKVEDGEEIT SEIAAAALRR SVHLFSALQA SDGHWCAENG 130 140 150 160 170 180 GLLFFLPPLV FAVYITGHLN TVFSPEHRKE ILRYIYCHQN EDGGWGIHIE GHSTMFCTVL 190 200 210 220 230 240 NYICMRILGE ARDGGIENAC ERGRKWILDH GGATGISSWG KTWLSILGVY EWDGTNPMPP 250 260 270 280 290 300 EFWAFPSSFP LHPAKMFCYC RITYMPMSYL YGKRFVGPIT PLILQIREEI YNEPYNKIKW 310 320 330 340 350 360 NSVRHLCAKE DNYFPHPTIQ KLLWDALYTF SEPLFSRWPF NKLREKALKI TMDHIHYEDH 370 380 390 400 410 420 NSRYITIGCV EKPLCMLACW IEDPHGEAFK KHLARIADYI WVGEDGIKMQ SFGSQTWDTS 430 440 450 460 470 480 LALQALIASD LSHEIGPTLK QGHVFTKNSQ ATENPSGDFR KMFRHISKGA WTFSDKDQGW 490 500 510 520 530 540 QVSDCTAESL KCCLLFSMMP PEIVGEKMEP EKVYDSVNVI LSLQSQNGGF TAWEPARAGS 550 560 570 580 590 600 WMEWLNPVEF MEDLVVEHEY VECTSSAIQA LVLFKKLYPR HRNKEIENCI INAAQFIENI 610 620 630 640 650 660 QEPDGSWYGN WGICFSYGTW FALKGLAAAG RTYENCSAIR KGVDFLLKSQ RDDGGWAESY 670 680 690 700 710 720 LSCPKKVYVP FEGNRSNLVQ TAWAMMGLIY GGQAKRDPMP LHRAAKLLIN SQTDLGDFPQ 730 740 750 760 QELTGAFMRN CMLHYALFRN TFPIWALAEY RRHVLFPSAG FGFGFTNNL

References

[1]

"Cloning and characterization of a lupeol synthase involved in the synthesis of epicuticular wax crystals on stem and hypocotyl surfaces of Ricinus communis." Guhling O., Hobl B., Yeats T., Jetter R. Arch. Biochem. Biophys. 448:60-72(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

Web resources

Protein Spotlight

Get a grip - Issue 135 of January 2012

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ268869 mRNA. Translation: ABB76766.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018333. Squalene_cyclase. IPR002365. Terpene_synthase_CS. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 2 hits.
TIGRFAMs	TIGR01787. squalene_cyclas. 1 hit.
PROSITE	PS01074. TERPENE_SYNTHASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUPS_RICCO

Accession

Primary (citable) accession number: Q2XPU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2011
Last sequence update:	December 20, 2005
Last modified:	January 9, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program