Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q2WGK3

- HOG1_TRIHA

UniProt

Q2WGK3 - HOG1_TRIHA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Mitogen-activated protein kinase hog1

Gene

hog1

Organism
Trichoderma harzianum (Hypocrea lixii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium.By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491ATPPROSITE-ProRule annotation
Active sitei141 – 1411Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 349ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase hog1 (EC:2.7.11.24)
Short name:
MAP kinase hog1
Alternative name(s):
Osmotic stress MAP kinase
ThHog1
Gene namesi
Name:hog1
Synonyms:osm1
OrganismiTrichoderma harzianum (Hypocrea lixii)
Taxonomic identifieri5544 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication
Note: Concentrates within the nucleus in response to hyperosmotic conditions and phosphorylation.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi315 – 3151F → S: Activates HOG1 in a constitutive manner. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Mitogen-activated protein kinase hog1PRO_0000289704Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei171 – 1711PhosphothreonineBy similarity
Modified residuei173 – 1731PhosphotyrosineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-171 and Tyr-173, which activates the enzyme (By similarity). Phosphorylated after osmotic stress.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliQ2WGK3.
SMRiQ2WGK3. Positions 3-342.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 299280Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi171 – 1733TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2WGK3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEFVRAQIF GTTFEITSRY SDLQPVGMGA FGLVCSARDQ LTNQNVAVKK
60 70 80 90 100
IMKPFSTPVL AKRTYRELKL LKHLRHENVI SLSDIFISPL EDIYFVTELL
110 120 130 140 150
GTDLHRLLTS RPLEKQFIQY FLYQIMRGLK YVHSAGVVHR DLKPSNILVN
160 170 180 190 200
ENCDLKICDF GLARIQDPQM TGYVSTRYYR APEIMLTWQK YDVEVDIWSA
210 220 230 240 250
GCIFAEMLEG KPLFPGKDHV NQFSIITELL GTPPDDVINT IASENTLRFV
260 270 280 290 300
KSLPKRERQP LRNKFKNADD SAIDLLERML VFDPKKRVTA TQALAHEYLQ
310 320 330 340 350
PYHDPTDEPV ADEKFDWSFN DADLPVDTWK IMMYSEILDY HNVEGAPNME

EQFPPQ
Length:356
Mass (Da):41,105
Last modified:January 10, 2006 - v1
Checksum:i3C7C246A24185FE7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB195837 Genomic DNA. Translation: BAE53434.1.
DQ071423 Genomic DNA. Translation: AAY82585.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB195837 Genomic DNA. Translation: BAE53434.1 .
DQ071423 Genomic DNA. Translation: AAY82585.1 .

3D structure databases

ProteinModelPortali Q2WGK3.
SMRi Q2WGK3. Positions 3-342.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Functional analysis of three MAP kinases of Trichoderma harzianum."
    Motoyama T., Ono D., Mukouzaka Y., Kawai Y., Kudo T.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of new molecular hallmarks for YSAPK MAPKs: application for cloning strategies in different fungal filamentous species."
    Adam A.L., Kohut G., Laday M.
    Acta Phytopathol. Entomol. Hung. 40:233-249(2005)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-253.
    Strain: G-1.
  3. "ThHog1 controls the hyperosmotic stress response in Trichoderma harzianum."
    Delgado-Jarana J., Sousa S., Gonzalez F., Rey M., Llobell A.
    Microbiology 152:1687-1700(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, MUTAGENESIS OF PHE-315.

Entry informationi

Entry nameiHOG1_TRIHA
AccessioniPrimary (citable) accession number: Q2WGK3
Secondary accession number(s): Q4PKS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: January 10, 2006
Last modified: October 29, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3