Reviewed, UniProtKB/Swiss-Prot Q2WGK3 (HOG1_TRIHA)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase hog1
      Short name=MAP kinase hog1
    EC=2.7.11.24
Alternative name(s):
    ThHog1
    Osmotic stress MAP kinase

Gene names

Name:	hog1
Synonyms:	osm1

Organism

Trichoderma harzianum (Hypocrea lixii)

Taxonomic identifier

5544 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Hypocreaceae › Hypocrea

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes. Ref.3
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation By similarity.
Subcellular location	Cytoplasm. Nucleus. Note: Concentrates within the nucleus in response to hyperosmotic conditions and phosphorylation. Ref.3
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-171 and Tyr-173, which activates the enzyme By similarity. Phosphorylated after osmotic stress. Ref.3
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Mitogen-activated protein kinase hog1		PRO_0000289704
Regions
	Domain	20 – 299	280	Protein kinase
	Nucleotide binding	26 – 34	9	ATP By similarity
	Motif	171 – 173	3	TXY
Sites
	Active site	141	1	Proton acceptor By similarity
	Binding site	49	1	ATP By similarity
Amino acid modifications
	Modified residue	171	1	Phosphothreonine By similarity
	Modified residue	173	1	Phosphotyrosine By similarity
Experimental info
	Mutagenesis	315	1	F → S: Activates HOG1 in a constitutive manner. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q2WGK3-1 [UniParc]. Last modified January 10, 2006. Version 1. Checksum: 3C7C246A24185FE7	FASTA	356	41,105
	10 20 30 40 50 60 MAEFVRAQIF GTTFEITSRY SDLQPVGMGA FGLVCSARDQ LTNQNVAVKK IMKPFSTPVL 70 80 90 100 110 120 AKRTYRELKL LKHLRHENVI SLSDIFISPL EDIYFVTELL GTDLHRLLTS RPLEKQFIQY 130 140 150 160 170 180 FLYQIMRGLK YVHSAGVVHR DLKPSNILVN ENCDLKICDF GLARIQDPQM TGYVSTRYYR 190 200 210 220 230 240 APEIMLTWQK YDVEVDIWSA GCIFAEMLEG KPLFPGKDHV NQFSIITELL GTPPDDVINT 250 260 270 280 290 300 IASENTLRFV KSLPKRERQP LRNKFKNADD SAIDLLERML VFDPKKRVTA TQALAHEYLQ 310 320 330 340 350 PYHDPTDEPV ADEKFDWSFN DADLPVDTWK IMMYSEILDY HNVEGAPNME EQFPPQ

References

[1]	"Functional analysis of three MAP kinases of Trichoderma harzianum." Motoyama T., Ono D., Mukouzaka Y., Kawai Y., Kudo T. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Identification of new molecular hallmarks for YSAPK MAPKs: application for cloning strategies in different fungal filamentous species." Adam A.L., Kohut G., Laday M. Acta Phytopathol. Entomol. Hung. 40:233-249(2005) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-253. Strain: G-1.
[3]	"ThHog1 controls the hyperosmotic stress response in Trichoderma harzianum." Delgado-Jarana J., Sousa S., Gonzalez F., Rey M., Llobell A. Microbiology 152:1687-1700(2006) [PubMed: 16735732] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, MUTAGENESIS OF PHE-315.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB195837 Genomic DNA. Translation: BAE53434.1. DQ071423 Genomic DNA. Translation: AAY82585.1.
3D structure databases
HSSP	HSSP built from PDB template 2GHL based on UniProtKB Q3U6R5.
SMR	Q2WGK3. Positions 3-342.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.24. 3745.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOG1_TRIHA

Accession

Primary (citable) accession number: Q2WGK3
Secondary accession number(s): Q4PKS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	January 10, 2006
Last modified:	January 19, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)