Q2WAJ8 (CHEB1_MAGSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase 1
EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	amb0323

Organism

Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) [Complete proteome] [HAMAP]

Taxonomic identifier

342108 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Magnetospirillum

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000264286
Regions
	Domain	14 – 132	119	Response regulatory
	Domain	188 – 381	194	CheB-type methylesterase
Sites
	Active site	199	1	By similarity
	Active site	227	1	By similarity
	Active site	323	1	By similarity
Amino acid modifications
	Modified residue	65	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2WAJ8 [UniParc]. Last modified January 10, 2006. Version 1. Checksum: BC671434000D5289	FASTA	381	39,765
	10 20 30 40 50 60 MAIDSPSPAT DSIRVMLVDD SAVVRGLVTR ILEGEAGIQV VASVGNGQMA LASLERNEID 70 80 90 100 110 120 VVILDIEMPV MDGLTALPKL LQIDPGLKVI MQSTLTLKGA DVSLRAMQMG AADYIPKPTS 130 140 150 160 170 180 TRDLAGGVDF KSELVTKIRA LGQARRAGAR PARPGGPPAT RPVIASTSPR TPVPIHPPGP 190 200 210 220 230 240 LQLRSNTPEP PDIIAIGSST GGPQALFTVL GTMKAGTVRQ PIVITQHMPA TFTTILAEHI 250 260 270 280 290 300 GRVSGWEARE AQDGEAIRGG RVYIAPGDFH MVVETKGTDK VLRLNKNPPE NFCRPAVDPM 310 320 330 340 350 360 LRSIAAAYGK RVLACILTGM GADGMKGGQA VVASGGTVIA QDEASSVVWG MPGAAATAGI 370 380 CSAVLPLPEI APWIMKLAAR R

References

[1]

"Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1." Matsunaga T., Okamura Y., Fukuda Y., Wahyudi A.T., Murase Y., Takeyama H. DNA Res. 12:157-166(2005) [PubMed: 16303747] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AMB-1 / ATCC 700264.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP007255 Genomic DNA. Translation: BAE49127.1.
RefSeq	YP_419686.1. NC_007626.1.
3D structure databases
ProteinModelPortal	Q2WAJ8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2WAJ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3806085.
GenomeReviews	Gene locus amb0323 in contig AP007255_GR.
KEGG	mag:amb0323.
NMPDR	fig|342108.5.peg.322.
PATRIC	22434916. VBIMagMag129836_0329.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	LISRWVE.
PhylomeDB	Q2WAJ8.
ProtClustDB	PRK00742.
Enzyme and pathway databases
BioCyc	MMAG342108:AMB0323-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_MAGSA

Accession

Primary (citable) accession number: Q2WAJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	January 10, 2006
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program