Q2W992 (QUEF_MAGSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	amb0779

Organism

Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) [Complete proteome] [HAMAP]

Taxonomic identifier

342108 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Magnetospirillum › Magnetospirillum magneticum

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP-Rule MF_00818
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP-Rule MF_00818
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00818
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 153	153	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP-Rule MF_00818		PRO_0000247682

Sequences

	Sequence		Length	Mass (Da)
	Q2W992 [UniParc]. Last modified January 10, 2006. Version 1. Checksum: A7F847BFFE5F9C3D	FASTA	153	16,520
	10 20 30 40 50 60 MGTGTEHLTQ LGQSTALPAS PDKAVLETVP NPHPGTLYLV RFTAPEFTSL CPITGQPDFA 70 80 90 100 110 120 QLVIDYAPEG SLVESKSLKL FLGSFRNHGA FHEDCTIAIA KRLVAACAPK WLRIGGYWYP 130 140 150 RGGIPIDVFW QTGPAPEGLW LPDQGVAGYR GRG

References

[1]

"Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1." Matsunaga T., Okamura Y., Fukuda Y., Wahyudi A.T., Murase Y., Takeyama H. DNA Res. 12:157-166(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AMB-1 / ATCC 700264.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP007255 Genomic DNA. Translation: BAE49583.1.
RefSeq	YP_420142.1. NC_007626.1.
3D structure databases
ProteinModelPortal	Q2W992.
ModBase	Search...
Protein-protein interaction databases
STRING	342108.amb0779.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAE49583; BAE49583; amb0779.
GeneID	3802158.
KEGG	mag:amb0779.
PATRIC	22435854. VBIMagMag129836_0791.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HOG000009527.
KO	K09457.
OMA	LECKEFT.
ProtClustDB	PRK13258.
Enzyme and pathway databases
BioCyc	MMAG342108:GJNU-787-MONOMER.
UniPathway	UPA00392.
Family and domain databases
HAMAP	MF_00818. QueF_type1.
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_MAGSA

Accession

Primary (citable) accession number: Q2W992

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	January 10, 2006
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program