Q2W7Y6 (SYE1_MAGSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	amb1235

Organism

Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) [Complete proteome] [HAMAP]

Taxonomic identifier

342108 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Magnetospirillum

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000237368
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022_B
	Motif	241 – 245	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	244	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2W7Y6 [UniParc]. Last modified January 10, 2006. Version 1. Checksum: 9A252B67868D01F6	FASTA	444	49,181
	10 20 30 40 50 60 MTSPVVRFAP SPTGLLHVGN ARVALLNRLF AKAHGGSFIL RLDDTDLERS RPEFAEAILD 70 80 90 100 110 120 DLRWLGLDWD RLERQSARLD AYAAAAERLK VSGRVYACYE TPDELELRRK VQLGRGLPPV 130 140 150 160 170 180 YDRAALKLAP DEIARLEAEG RRPHWRFKLE LEDVRWDDCV RGPSHYHGGN LSDPVLIRGD 190 200 210 220 230 240 GSFLYTLPSV VDDIEFGVTH IIRGEDHVTN TAPQIQLFQA LGAVPPAFAH LPLLTGATGE 250 260 270 280 290 300 GLSKRLGSGS LKDLRATGIH PMALNSLLAK LGSSDAIDIR HTLAELEAEF AWDKFGRGTP 310 320 330 340 350 360 KFDSAELERL DARLMHTASF AEVRDRLGID GADEEFWLAV RPNLNHLAEA EAWWAICRQD 370 380 390 400 410 420 LTPVIEDGEF TRAAAALLPD GAWDHATWGA WTETVKQATG RKGKALFHPL RLALTGRENG 430 440 PELKTLLPLI GHDRARARLM GEIA

References

[1]

"Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1." Matsunaga T., Okamura Y., Fukuda Y., Wahyudi A.T., Murase Y., Takeyama H. DNA Res. 12:157-166(2005) [PubMed: 16303747] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AMB-1 / ATCC 700264.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP007255 Genomic DNA. Translation: BAE50039.1.
RefSeq	YP_420598.1. NC_007626.1.
3D structure databases
ProteinModelPortal	Q2W7Y6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2W7Y6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3803454.
GenomeReviews	Gene locus amb1235 in contig AP007255_GR.
KEGG	mag:amb1235.
NMPDR	fig|342108.5.peg.1094.
PATRIC	22436731. VBIMagMag129836_1221.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DQERIEW.
ProtClustDB	PRK12558.
Enzyme and pathway databases
BioCyc	MMAG342108:AMB1235-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_MAGSA

Accession

Primary (citable) accession number: Q2W7Y6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	January 10, 2006
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program