Reviewed, UniProtKB/Swiss-Prot Q2W5V5 (CHEB2_MAGSA)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 2
    EC=3.1.1.61

Gene names

Name:	cheB2
Ordered Locus Names:	amb1966

Organism

Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) [Complete proteome] [HAMAP]

Taxonomic identifier

342108 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Magnetospirillum

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Chemotaxis response regulator protein-glutamate methylesterase 2 HAMAP MF_00099		PRO_0000264287
Regions
	Domain	6 – 124	119	Response regulatory
	Domain	162 – 350	189	CheB-type methylesterase
Sites
	Active site	173	1	By similarity
	Active site	200	1	By similarity
	Active site	292	1	By similarity
Amino acid modifications
	Modified residue	57	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2W5V5-1 [UniParc]. Last modified January 10, 2006. Version 1. Checksum: B7E7ECF3575EF499	FASTA	352	37,022
	10 20 30 40 50 60 MRKKIKVLIV EDSLVVRELL KHIIGSDERF EVMAAVTSAE DCLEMLETQQ PDVISLDIRL 70 80 90 100 110 120 PGMNGLDATL KIMSRRPTPI VVVAAQVDDN ELNIAMNALR AGALSVVEKP VGVTNAGYDT 130 140 150 160 170 180 MAAKICTQLA IMSQVQVVRQ GINRGLNFGS DDTPARVSQG RPGTYSMVGI VASTGGPQAL 190 200 210 220 230 240 VQLLGGLGAD FPLPILLVQH ITSSFLEGFV TWLSGTTPFE ARIAQDGEKP VAGKVYVAPV 250 260 270 280 290 300 DHHLGLVNDQ LVILDLPAVC NQKPSGTVLF GSMARDIGKH GIGVVLTGMG ADGSEGLRQM 310 320 330 340 350 ADKGAYTIVE DASTCVVNGM PAAAAKLGAA RETLPLPAIA ARLRDLALGG EK

References

[1]

"Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1." Matsunaga T., Okamura Y., Fukuda Y., Wahyudi A.T., Murase Y., Takeyama H. DNA Res. 12:157-166(2005) [PubMed: 16303747] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP007255 Genomic DNA. Translation: BAE50770.1.
RefSeq	YP_421329.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2W5V5.
Genome annotation databases
GeneID	3804483.
GenomeReviews	Gene locus amb1966 in contig AP007255_GR.
KEGG	mag:amb1966.
NMPDR	fig|342108.5.peg.1726.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2W5V5.
OMA	TTRRIMA.
Enzyme and pathway databases
BioCyc	MMAG342108:AMB1966-MON.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB2_MAGSA

Accession

Primary (citable) accession number: Q2W5V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	January 10, 2006
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)