Reviewed,
UniProtKB/Swiss-Prot Q2W3T1 (NAPA_MAGMM)
Last modified
November 25, 2008.
Version 30.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Periplasmic nitrate reductase EC=1.7.99.4 | ||||
| Gene names |
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| Organism | Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 342108 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Magnetospirillum |
Protein attributes
| Sequence length | 827 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. |
| Catalytic activity | Nitrite + acceptor = nitrate + reduced acceptor. |
| Cofactor | Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity. |
| Subunit structure | Interacts with napB By similarity. |
| Subcellular location | PeriplasmBy similarity. |
| Post-translational modification | Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. |
| Sequence similarities | Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 34 | 34 | Tat-type signal Potential | ||||||
| Chain | 35 – 827 | 793 | Periplasmic nitrate reductase | PRO_0000256074 | |||||
Sites | |||||||||
| Metal binding | 43 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 46 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 50 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 78 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
Sequences
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References
| [1] | "Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1." Matsunaga T., Okamura Y., Fukuda Y., Wahyudi A.T., Murase Y., Takeyama H. DNA Res. 12:157-166(2005) [PubMed: 16303747] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| AP007255 Genomic DNA. Translation: BAE51494.1. | |
| RefSeq | YP_422053.1. |
3D structure databases | |
| SMR | Q2W3T1. Positions 36-826. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 3802787. |
| GenomeReviews | Gene locus amb2690 in contig AP007255_GR. |
| KEGG | mag:amb2690. |
| NMPDR | fig|342108.5.peg.2385. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | Q2W3T1. |
Enzyme and pathway databases | |
| BioCyc | MMAG342108:AMB2690-MON. |
Family and domain databases | |
| HAMAP | MF_01630. [Tree] |
| InterPro | IPR009010. Asp_de-COase-like_fold. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. Tat. [Graphical view] |
| Gene3D | G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit. |
| Pfam | PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit. |
| PROSITE | PS00551. MOLYBDOPTERIN_PROK_1. False negative. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NAPA_MAGMM | ||||||||
| Accession | Primary (citable) accession number: Q2W3T1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||

Clusters with


