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Q2W3T1

- NAPA_MAGSA

UniProt

Q2W3T1 - NAPA_MAGSA

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Protein

Periplasmic nitrate reductase

Gene
napA, amb2690
Organism
Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431Iron-sulfur (4Fe-4S) By similarity
Metal bindingi46 – 461Iron-sulfur (4Fe-4S) By similarity
Metal bindingi50 – 501Iron-sulfur (4Fe-4S) By similarity
Metal bindingi78 – 781Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciMMAG342108:GJNU-2720-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:amb2690
OrganismiMagnetospirillum magneticum (strain AMB-1 / ATCC 700264)
Taxonomic identifieri342108 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeMagnetospirillum
ProteomesiUP000007058: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434Tat-type signal Reviewed predictionAdd
BLAST
Chaini35 – 827793Periplasmic nitrate reductaseUniRule annotationPRO_0000256074Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi342108.amb2690.

Structurei

3D structure databases

ProteinModelPortaliQ2W3T1.
SMRiQ2W3T1. Positions 36-826.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 92574Fe-4S Mo/W bis-MGD-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiCACKVER.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2W3T1-1 [UniParc]FASTAAdd to Basket

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MSLTRRDFIK ANAVAATAAA AGIATPAIAQ PAKANIRWDK GVCRFCGTGC    50
AVLVGVQDGR VVATQGDPDS PVNRGLNCIK GYFLSKIMYG EDRLTKPLLR 100
MKNGKFDKNG EFQPISWDQA FDIMAEKWKE QLKKPDGVTR VGMFGSGQWT 150
IWEGYAASKL FKAGFRSNNL DPNARHCMAS AVAGFMRTFG IDEPMGCYDD 200
IEQTDAFVLW GSNMAEMHPI LWSRVTDRRL THEGCKVAVL STFEHRSFEL 250
ADIPMVFTPQ TDLAILNYIC HYIISKNAYD KDFIEKHVNF KKGATDIGYG 300
LRPTHALEKD QANAATPDKS DPMTFDEFKA FVAEYTLEKV AKLSGVPADK 350
LEALAKLYAD PKVKVVSFWT MGFNQHARGT WVNNMIYNVH LLMGKISEPG 400
NSPFSLTGQP SACGTAREVG TFAHRLPADM VVMNDKHREI TEGLWKLPPG 450
TLNPKIGYHA VLQHRMLKDG KLNAYWVMCT NNMQTAPNMN EEGYPGYRNP 500
ANFIVVSDPY PTVTALAADL ILPTAMWMEK EGAYGNAERR TQFWRQQVKA 550
PGEAKSDLWQ IMEFSKRFKI EEVWPEELLA KKPELRGKTL FDVLYKNGQV 600
DKFPATDIQA GFENDEAKAF GFYPQKGLFE EYASFGRGHA HDLAPFEAYH 650
KARGLRWPVV DGKETLWRFR EGYDPYVKAG EGVKFYGKPD GKAWIFALPY 700
QPAAESPDKE FDLWLSTGRV LEHWHSGSMT RRVPELHKSV PNAVLYMHPN 750
DAAKRNLRNG DVVKVASRRG EVTTRIDTRG RNKPPEGLVF MPFFDESQLV 800
NKLTLDATCP ISKETDYKKC AVKVSKA 827
Length:827
Mass (Da):92,939
Last modified:January 10, 2006 - v1
Checksum:i1F1DE75CBE2CFC6C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP007255 Genomic DNA. Translation: BAE51494.1.
RefSeqiYP_422053.1. NC_007626.1.

Genome annotation databases

EnsemblBacteriaiBAE51494; BAE51494; amb2690.
GeneIDi3802787.
KEGGimag:amb2690.
PATRICi22439634. VBIMagMag129836_2656.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP007255 Genomic DNA. Translation: BAE51494.1 .
RefSeqi YP_422053.1. NC_007626.1.

3D structure databases

ProteinModelPortali Q2W3T1.
SMRi Q2W3T1. Positions 36-826.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 342108.amb2690.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAE51494 ; BAE51494 ; amb2690 .
GeneIDi 3802787.
KEGGi mag:amb2690.
PATRICi 22439634. VBIMagMag129836_2656.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi CACKVER.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci MMAG342108:GJNU-2720-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1."
    Matsunaga T., Okamura Y., Fukuda Y., Wahyudi A.T., Murase Y., Takeyama H.
    DNA Res. 12:157-166(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AMB-1 / ATCC 700264.

Entry informationi

Entry nameiNAPA_MAGSA
AccessioniPrimary (citable) accession number: Q2W3T1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: January 10, 2006
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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