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Q2W3T1 (NAPA_MAGSA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:amb2690
OrganismMagnetospirillum magneticum (strain AMB-1 / ATCC 700264) [Complete proteome] [HAMAP]
Taxonomic identifier342108 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeMagnetospirillum

Protein attributes

Sequence length827 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434Tat-type signal Potential
Chain35 – 827793Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000256074

Regions

Domain36 – 92574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding431Iron-sulfur (4Fe-4S) By similarity
Metal binding461Iron-sulfur (4Fe-4S) By similarity
Metal binding501Iron-sulfur (4Fe-4S) By similarity
Metal binding781Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2W3T1 [UniParc].

Last modified January 10, 2006. Version 1.
Checksum: 1F1DE75CBE2CFC6C

FASTA82792,939
        10         20         30         40         50         60 
MSLTRRDFIK ANAVAATAAA AGIATPAIAQ PAKANIRWDK GVCRFCGTGC AVLVGVQDGR 

        70         80         90        100        110        120 
VVATQGDPDS PVNRGLNCIK GYFLSKIMYG EDRLTKPLLR MKNGKFDKNG EFQPISWDQA 

       130        140        150        160        170        180 
FDIMAEKWKE QLKKPDGVTR VGMFGSGQWT IWEGYAASKL FKAGFRSNNL DPNARHCMAS 

       190        200        210        220        230        240 
AVAGFMRTFG IDEPMGCYDD IEQTDAFVLW GSNMAEMHPI LWSRVTDRRL THEGCKVAVL 

       250        260        270        280        290        300 
STFEHRSFEL ADIPMVFTPQ TDLAILNYIC HYIISKNAYD KDFIEKHVNF KKGATDIGYG 

       310        320        330        340        350        360 
LRPTHALEKD QANAATPDKS DPMTFDEFKA FVAEYTLEKV AKLSGVPADK LEALAKLYAD 

       370        380        390        400        410        420 
PKVKVVSFWT MGFNQHARGT WVNNMIYNVH LLMGKISEPG NSPFSLTGQP SACGTAREVG 

       430        440        450        460        470        480 
TFAHRLPADM VVMNDKHREI TEGLWKLPPG TLNPKIGYHA VLQHRMLKDG KLNAYWVMCT 

       490        500        510        520        530        540 
NNMQTAPNMN EEGYPGYRNP ANFIVVSDPY PTVTALAADL ILPTAMWMEK EGAYGNAERR 

       550        560        570        580        590        600 
TQFWRQQVKA PGEAKSDLWQ IMEFSKRFKI EEVWPEELLA KKPELRGKTL FDVLYKNGQV 

       610        620        630        640        650        660 
DKFPATDIQA GFENDEAKAF GFYPQKGLFE EYASFGRGHA HDLAPFEAYH KARGLRWPVV 

       670        680        690        700        710        720 
DGKETLWRFR EGYDPYVKAG EGVKFYGKPD GKAWIFALPY QPAAESPDKE FDLWLSTGRV 

       730        740        750        760        770        780 
LEHWHSGSMT RRVPELHKSV PNAVLYMHPN DAAKRNLRNG DVVKVASRRG EVTTRIDTRG 

       790        800        810        820 
RNKPPEGLVF MPFFDESQLV NKLTLDATCP ISKETDYKKC AVKVSKA 

« Hide

References

[1]"Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1."
Matsunaga T., Okamura Y., Fukuda Y., Wahyudi A.T., Murase Y., Takeyama H.
DNA Res. 12:157-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AMB-1 / ATCC 700264.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP007255 Genomic DNA. Translation: BAE51494.1.
RefSeqYP_422053.1. NC_007626.1.

3D structure databases

ProteinModelPortalQ2W3T1.
SMRQ2W3T1. Positions 36-826.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING342108.amb2690.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAE51494; BAE51494; amb2690.
GeneID3802787.
KEGGmag:amb2690.
PATRIC22439634. VBIMagMag129836_2656.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMACACKVER.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycMMAG342108:GJNU-2720-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_MAGSA
AccessionPrimary (citable) accession number: Q2W3T1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: January 10, 2006
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families