Reviewed, UniProtKB/Swiss-Prot Q2W3T1 (NAPA_MAGMM)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	amb2690

Organism

Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) [Complete proteome] [HAMAP]

Taxonomic identifier

342108 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Magnetospirillum

Protein attributes

Sequence length	827 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with napB By similarity.
Subcellular location	PeriplasmBy similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Tat-type signal Potential
	Chain	35 – 827	793	Periplasmic nitrate reductase		PRO_0000256074
Sites
	Metal binding	43	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	46	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	50	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	78	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2W3T1-1 [UniParc]. Last modified January 10, 2006. Version 1. Checksum: 1F1DE75CBE2CFC6C	FASTA	827	92,939
	10 20 30 40 50 60 MSLTRRDFIK ANAVAATAAA AGIATPAIAQ PAKANIRWDK GVCRFCGTGC AVLVGVQDGR 70 80 90 100 110 120 VVATQGDPDS PVNRGLNCIK GYFLSKIMYG EDRLTKPLLR MKNGKFDKNG EFQPISWDQA 130 140 150 160 170 180 FDIMAEKWKE QLKKPDGVTR VGMFGSGQWT IWEGYAASKL FKAGFRSNNL DPNARHCMAS 190 200 210 220 230 240 AVAGFMRTFG IDEPMGCYDD IEQTDAFVLW GSNMAEMHPI LWSRVTDRRL THEGCKVAVL 250 260 270 280 290 300 STFEHRSFEL ADIPMVFTPQ TDLAILNYIC HYIISKNAYD KDFIEKHVNF KKGATDIGYG 310 320 330 340 350 360 LRPTHALEKD QANAATPDKS DPMTFDEFKA FVAEYTLEKV AKLSGVPADK LEALAKLYAD 370 380 390 400 410 420 PKVKVVSFWT MGFNQHARGT WVNNMIYNVH LLMGKISEPG NSPFSLTGQP SACGTAREVG 430 440 450 460 470 480 TFAHRLPADM VVMNDKHREI TEGLWKLPPG TLNPKIGYHA VLQHRMLKDG KLNAYWVMCT 490 500 510 520 530 540 NNMQTAPNMN EEGYPGYRNP ANFIVVSDPY PTVTALAADL ILPTAMWMEK EGAYGNAERR 550 560 570 580 590 600 TQFWRQQVKA PGEAKSDLWQ IMEFSKRFKI EEVWPEELLA KKPELRGKTL FDVLYKNGQV 610 620 630 640 650 660 DKFPATDIQA GFENDEAKAF GFYPQKGLFE EYASFGRGHA HDLAPFEAYH KARGLRWPVV 670 680 690 700 710 720 DGKETLWRFR EGYDPYVKAG EGVKFYGKPD GKAWIFALPY QPAAESPDKE FDLWLSTGRV 730 740 750 760 770 780 LEHWHSGSMT RRVPELHKSV PNAVLYMHPN DAAKRNLRNG DVVKVASRRG EVTTRIDTRG 790 800 810 820 RNKPPEGLVF MPFFDESQLV NKLTLDATCP ISKETDYKKC AVKVSKA

References

[1]

"Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1." Matsunaga T., Okamura Y., Fukuda Y., Wahyudi A.T., Murase Y., Takeyama H. DNA Res. 12:157-166(2005) [PubMed: 16303747] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP007255 Genomic DNA. Translation: BAE51494.1.
RefSeq	YP_422053.1.
3D structure databases
SMR	Q2W3T1. Positions 36-826.
ModBase	Search...
Genome annotation databases
GeneID	3802787.
GenomeReviews	Gene locus amb2690 in contig AP007255_GR.
KEGG	mag:amb2690.
NMPDR	fig|342108.5.peg.2385.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2W3T1.
Enzyme and pathway databases
BioCyc	MMAG342108:AMB2690-MON.
Family and domain databases
HAMAP	MF_01630. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. False negative. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_MAGMM

Accession

Primary (citable) accession number: Q2W3T1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	January 10, 2006
Last modified:	November 25, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)