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Q2W3T1

- NAPA_MAGSA

UniProt

Q2W3T1 - NAPA_MAGSA

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (10 Jan 2006)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi43 – 431Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi78 – 781Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciMMAG342108:GJNU-2720-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:amb2690
    OrganismiMagnetospirillum magneticum (strain AMB-1 / ATCC 700264)
    Taxonomic identifieri342108 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeMagnetospirillum
    ProteomesiUP000007058: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3434Tat-type signalUniRule annotationAdd
    BLAST
    Chaini35 – 827793Periplasmic nitrate reductasePRO_0000256074Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi342108.amb2690.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2W3T1.
    SMRiQ2W3T1. Positions 36-826.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini36 – 92574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiCACKVER.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q2W3T1-1 [UniParc]FASTAAdd to Basket

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    MSLTRRDFIK ANAVAATAAA AGIATPAIAQ PAKANIRWDK GVCRFCGTGC    50
    AVLVGVQDGR VVATQGDPDS PVNRGLNCIK GYFLSKIMYG EDRLTKPLLR 100
    MKNGKFDKNG EFQPISWDQA FDIMAEKWKE QLKKPDGVTR VGMFGSGQWT 150
    IWEGYAASKL FKAGFRSNNL DPNARHCMAS AVAGFMRTFG IDEPMGCYDD 200
    IEQTDAFVLW GSNMAEMHPI LWSRVTDRRL THEGCKVAVL STFEHRSFEL 250
    ADIPMVFTPQ TDLAILNYIC HYIISKNAYD KDFIEKHVNF KKGATDIGYG 300
    LRPTHALEKD QANAATPDKS DPMTFDEFKA FVAEYTLEKV AKLSGVPADK 350
    LEALAKLYAD PKVKVVSFWT MGFNQHARGT WVNNMIYNVH LLMGKISEPG 400
    NSPFSLTGQP SACGTAREVG TFAHRLPADM VVMNDKHREI TEGLWKLPPG 450
    TLNPKIGYHA VLQHRMLKDG KLNAYWVMCT NNMQTAPNMN EEGYPGYRNP 500
    ANFIVVSDPY PTVTALAADL ILPTAMWMEK EGAYGNAERR TQFWRQQVKA 550
    PGEAKSDLWQ IMEFSKRFKI EEVWPEELLA KKPELRGKTL FDVLYKNGQV 600
    DKFPATDIQA GFENDEAKAF GFYPQKGLFE EYASFGRGHA HDLAPFEAYH 650
    KARGLRWPVV DGKETLWRFR EGYDPYVKAG EGVKFYGKPD GKAWIFALPY 700
    QPAAESPDKE FDLWLSTGRV LEHWHSGSMT RRVPELHKSV PNAVLYMHPN 750
    DAAKRNLRNG DVVKVASRRG EVTTRIDTRG RNKPPEGLVF MPFFDESQLV 800
    NKLTLDATCP ISKETDYKKC AVKVSKA 827
    Length:827
    Mass (Da):92,939
    Last modified:January 10, 2006 - v1
    Checksum:i1F1DE75CBE2CFC6C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP007255 Genomic DNA. Translation: BAE51494.1.
    RefSeqiYP_422053.1. NC_007626.1.

    Genome annotation databases

    EnsemblBacteriaiBAE51494; BAE51494; amb2690.
    GeneIDi3802787.
    KEGGimag:amb2690.
    PATRICi22439634. VBIMagMag129836_2656.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP007255 Genomic DNA. Translation: BAE51494.1 .
    RefSeqi YP_422053.1. NC_007626.1.

    3D structure databases

    ProteinModelPortali Q2W3T1.
    SMRi Q2W3T1. Positions 36-826.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 342108.amb2690.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAE51494 ; BAE51494 ; amb2690 .
    GeneIDi 3802787.
    KEGGi mag:amb2690.
    PATRICi 22439634. VBIMagMag129836_2656.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi CACKVER.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci MMAG342108:GJNU-2720-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1."
      Matsunaga T., Okamura Y., Fukuda Y., Wahyudi A.T., Murase Y., Takeyama H.
      DNA Res. 12:157-166(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AMB-1 / ATCC 700264.

    Entry informationi

    Entry nameiNAPA_MAGSA
    AccessioniPrimary (citable) accession number: Q2W3T1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: January 10, 2006
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3