Q2W2H3 (EFTU_MAGSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor Tu
Short name=EF-Tu

Gene names

Name:	tuf1
Ordered Locus Names:	amb3132
AND
Name:	tuf2
Ordered Locus Names:	amb3148

Organism

Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) [Complete proteome] [HAMAP]

Taxonomic identifier

342108 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Magnetospirillum › Magnetospirillum magneticum

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis By similarity. HAMAP-Rule MF_00118
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Elongation factor Tu HAMAP-Rule MF_00118		PRO_0000337427
Regions
	Nucleotide binding	19 – 26	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	136 – 139	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2W2H3 [UniParc]. Last modified January 10, 2006. Version 1. Checksum: 9BA6343579D588CA	FASTA	396	43,053
	10 20 30 40 50 60 MAKAKFERNK PHCNIGTIGH VDHGKTSLTA AITKILAETG GATFTAYDQI DKAPEEKARG 70 80 90 100 110 120 ITISTAHVEY ETSNRHYAHV DCPGHADYVK NMITGAAQMD GAILVVSAAD GPMPQTREHI 130 140 150 160 170 180 LLARQVGVPA LVVFMNKCDM VDDPELLDLV ELEVRELLSS YDFPGDDIPI VRGSALCALE 190 200 210 220 230 240 DKQPEIGRDA ILKLMAEVDA YIPQPERPKD KPFLMPIEDV FSISGRGTVV TGRVERGVVK 250 260 270 280 290 300 VGEEVEIVGI KNTVKTTCTG VEMFRKLLDQ GEAGDNIGAL LRGTKREDVE RGQVLAAPGS 310 320 330 340 350 360 ITPHTDFEAE AYILNKEEGG RHTPFFTNYR PQFYFRTTDV TGVVALPEGT EMVMPGDNVK 370 380 390 MIVTLIAPIA MDQGLRFAIR EGGRTVGAGV VAKIIK

References

[1]

"Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1." Matsunaga T., Okamura Y., Fukuda Y., Wahyudi A.T., Murase Y., Takeyama H. DNA Res. 12:157-166(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AMB-1 / ATCC 700264.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP007255 Genomic DNA. Translation: BAE51936.1. AP007255 Genomic DNA. Translation: BAE51952.1.
RefSeq	YP_422495.1. NC_007626.1. YP_422511.1. NC_007626.1.
3D structure databases
HSSP	HSSP built from PDB template 2BVN based on UniProtKB P0A6N1.
ProteinModelPortal	Q2W2H3.
SMR	Q2W2H3. Positions 2-395.
ModBase	Search...
Protein-protein interaction databases
STRING	342108.amb3148.
Proteomic databases
PRIDE	Q2W2H3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAE51936; BAE51936; amb3132. BAE51952; BAE51952; amb3148.
GeneID	3804789. 3804890.
KEGG	mag:amb3132. mag:amb3148.
PATRIC	22440559. VBIMagMag129836_3113.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0050.
HOGENOM	HOG000229290.
KO	K02358.
OMA	HTDFEAN.
ProtClustDB	PRK00049.
Enzyme and pathway databases
BioCyc	MMAG342108:GJNU-3167-MONOMER. MMAG342108:GJNU-3184-MONOMER.
Family and domain databases
HAMAP	MF_00118_B. EF_Tu_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR005225. Small_GTP-bd_dom. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004541. Transl_elong_EFTu/EF1A_bac/org. IPR004160. Transl_elong_EFTu/EF1A_C. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF50465. Elong_init_C. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00485. EF-Tu. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFTU_MAGSA

Accession

Primary (citable) accession number: Q2W2H3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	January 10, 2006
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program