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Protein

Adenylosuccinate synthetase

Gene

purA

Organism
Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.UniRule annotation

Catalytic activityi

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: AMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from IMP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Adenylosuccinate synthetase (purA)
  2. Adenylosuccinate lyase (amb3052)
This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei13 – 131Proton acceptorUniRule annotation
Metal bindingi13 – 131MagnesiumUniRule annotation
Metal bindingi40 – 401Magnesium; via carbonyl oxygenUniRule annotation
Active sitei41 – 411Proton donorUniRule annotation
Binding sitei129 – 1291IMPUniRule annotation
Binding sitei143 – 1431IMP; shared with dimeric partnerUniRule annotation
Binding sitei223 – 2231IMPUniRule annotation
Binding sitei238 – 2381IMPUniRule annotation
Binding sitei302 – 3021IMPUniRule annotation
Binding sitei304 – 3041GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 187GTPUniRule annotation
Nucleotide bindingi40 – 423GTPUniRule annotation
Nucleotide bindingi330 – 3323GTPUniRule annotation
Nucleotide bindingi412 – 4143GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMAG342108:GJNU-3230-MONOMER.
UniPathwayiUPA00075; UER00335.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylosuccinate synthetaseUniRule annotation (EC:6.3.4.4UniRule annotation)
Short name:
AMPSaseUniRule annotation
Short name:
AdSSUniRule annotation
Alternative name(s):
IMP--aspartate ligaseUniRule annotation
Gene namesi
Name:purAUniRule annotation
Ordered Locus Names:amb3190
OrganismiMagnetospirillum magneticum (strain AMB-1 / ATCC 700264)
Taxonomic identifieri342108 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeMagnetospirillum
Proteomesi
  • UP000007058 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429Adenylosuccinate synthetasePRO_1000000852Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi342108.amb3190.

Structurei

3D structure databases

ProteinModelPortaliQ2W2D1.
SMRiQ2W2D1. Positions 2-428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni13 – 164IMP bindingUniRule annotation
Regioni38 – 414IMP bindingUniRule annotation
Regioni298 – 3047Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the adenylosuccinate synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C91. Bacteria.
COG0104. LUCA.
HOGENOMiHOG000260959.
KOiK01939.
OMAiILPLHRE.
OrthoDBiPOG091H01G9.

Family and domain databases

HAMAPiMF_00011. Adenylosucc_synth. 1 hit.
InterProiIPR018220. Adenylosuccin_syn_GTP-bd.
IPR001114. Adenylosuccinate_synthetase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTiSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00184. purA. 1 hit.
PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2W2D1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANVAVVGAQ WGDEGKGKVV DWLSERADVV VRFQGGHNAG HTLVINGVTY
60 70 80 90 100
KLSLLPSGVV RGKLSIIGNG VVIDPWALLA EIERIRAQGV EITPEVLKIA
110 120 130 140 150
ENACLILPLH AHLDKAREEA SGTAKIGTTG RGIGPAYEDR VGRRAIRVCD
160 170 180 190 200
LADEAVLKAK IATLLRHHNA LLRGLGAAEV DGAELLAKLL DVAPKILPFA
210 220 230 240 250
DVVWQHLDEV RHTRKRVLFE GAQGAMLDVD HGTYPYVTSS NTVSGNAGTG
260 270 280 290 300
SGVGPGQVGY VLGICKAYTT RVGEGPFPTE LFDEIGKSIG ERGHEFGTVT
310 320 330 340 350
GRPRRCGWFD AVMVRQAVKV GGITGIALTK LDVLDGFKEL KICTGYKLDG
360 370 380 390 400
KVINHFPASM TGQANVEPIY EVIEGWSQST RGVRSWKDLP ATAIKYIRRI
410 420
EELIEAPVAL LSTSPEREDS ILVHDPFAT
Length:429
Mass (Da):46,135
Last modified:January 10, 2006 - v1
Checksum:iDBF56FF196968676
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007255 Genomic DNA. Translation: BAE51994.1.
RefSeqiWP_011385555.1. NC_007626.1.

Genome annotation databases

EnsemblBacteriaiBAE51994; BAE51994; amb3190.
KEGGimag:amb3190.
PATRICi22440683. VBIMagMag129836_3170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007255 Genomic DNA. Translation: BAE51994.1.
RefSeqiWP_011385555.1. NC_007626.1.

3D structure databases

ProteinModelPortaliQ2W2D1.
SMRiQ2W2D1. Positions 2-428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi342108.amb3190.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAE51994; BAE51994; amb3190.
KEGGimag:amb3190.
PATRICi22440683. VBIMagMag129836_3170.

Phylogenomic databases

eggNOGiENOG4105C91. Bacteria.
COG0104. LUCA.
HOGENOMiHOG000260959.
KOiK01939.
OMAiILPLHRE.
OrthoDBiPOG091H01G9.

Enzyme and pathway databases

UniPathwayiUPA00075; UER00335.
BioCyciMMAG342108:GJNU-3230-MONOMER.

Family and domain databases

HAMAPiMF_00011. Adenylosucc_synth. 1 hit.
InterProiIPR018220. Adenylosuccin_syn_GTP-bd.
IPR001114. Adenylosuccinate_synthetase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTiSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00184. purA. 1 hit.
PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPURA_MAGSA
AccessioniPrimary (citable) accession number: Q2W2D1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 10, 2006
Last modified: September 7, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.