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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7Magnesium1
Metal bindingi67Magnesium1
Metal bindingi139Magnesium1
Metal bindingi142Magnesium1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvC (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene namesi
Name:ruvC
Ordered Locus Names:amb3219
OrganismiMagnetospirillum magneticum (strain AMB-1 / ATCC 700264)
Taxonomic identifieri342108 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeMagnetospirillum
Proteomesi
  • UP000007058 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000027731 – 166Crossover junction endodeoxyribonuclease RuvCAdd BLAST166

Interactioni

Protein-protein interaction databases

STRINGi342108.amb3219.

Structurei

3D structure databases

ProteinModelPortaliQ2W2A2.
SMRiQ2W2A2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.

Phylogenomic databases

eggNOGiENOG4105NHU. Bacteria.
COG0817. LUCA.
HOGENOMiHOG000012181.
KOiK01159.
OMAiGLTRCGI.
OrthoDBiPOG091H0202.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC. 1 hit.
InterProiView protein in InterPro
IPR012337. RNaseH-like_sf.
IPR036397. RNaseH_sf.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
PANTHERiPTHR30194. PTHR30194. 1 hit.
PfamiView protein in Pfam
PF02075. RuvC. 1 hit.
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiView protein in PROSITE
PS01321. RUVC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2W2A2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILGLDPGL RNTGWGIIDA VDNRLRHVAD GVIRPDASAA LAERLVQLHD
60 70 80 90 100
GIMAVIADFS PDEAAVEETF VNMNPASTLK LGQARGVVLL VPAKSGLPVG
110 120 130 140 150
EYAATLVKQS VVGTGRAAKE QVGMMVKTLL PGCLAATADA ADALAVAICH
160
AHHRGTQQKL ARAVAR
Length:166
Mass (Da):17,302
Last modified:January 10, 2006 - v1
Checksum:i3D4ECC934C9D9013
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007255 Genomic DNA. Translation: BAE52023.1.
RefSeqiWP_011385584.1. NC_007626.1.

Genome annotation databases

EnsemblBacteriaiBAE52023; BAE52023; amb3219.
KEGGimag:amb3219.

Similar proteinsi

Entry informationi

Entry nameiRUVC_MAGSA
AccessioniPrimary (citable) accession number: Q2W2A2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 10, 2006
Last modified: October 25, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families