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Q2W0H0 (DDL_MAGSA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:amb3851
OrganismMagnetospirillum magneticum (strain AMB-1 / ATCC 700264) [Complete proteome] [HAMAP]
Taxonomic identifier342108 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeMagnetospirillum

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 305305D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341127

Regions

Domain104 – 300197ATP-grasp
Nucleotide binding131 – 18151ATP By similarity

Sites

Metal binding2491Magnesium or manganese 1 By similarity
Metal binding2671Magnesium or manganese 1 By similarity
Metal binding2671Magnesium or manganese 2 By similarity
Metal binding2691Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2W0H0 [UniParc].

Last modified January 10, 2006. Version 1.
Checksum: 0C672F815E884A21

FASTA30532,630
        10         20         30         40         50         60 
MSKRVTVLMG GASAERDVSL RSGAAAAKAL EQAGFEVALV DCDRNPAELV RAITETRPDV 

        70         80         90        100        110        120 
VFNALHGRFG EDGCVQGVLN LLGVPYTHSG LLASAAAMDK AFARALFASA GIPVAEGRVI 

       130        140        150        160        170        180 
TRTDRNGPDP LPRPFVVKPL NEGSSVGVFI VRDNQPSPLP DWPFDADEVL VESFIPGREL 

       190        200        210        220        230        240 
TAAVMGDRAL GVLEITSDHG FYDYEAKYAP GGSRHLMPAP IPEADYAEAC RLAVAAHKAL 

       250        260        270        280        290        300 
GCRGVSRADL RYDDTVPGQP PRLVMLEVNT QPGMTATSLV PEMAAYQGIT FPELVRWMVE 


EARCD 

« Hide

References

[1]"Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1."
Matsunaga T., Okamura Y., Fukuda Y., Wahyudi A.T., Murase Y., Takeyama H.
DNA Res. 12:157-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AMB-1 / ATCC 700264.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP007255 Genomic DNA. Translation: BAE52655.1.
RefSeqYP_423214.1. NC_007626.1.

3D structure databases

ProteinModelPortalQ2W0H0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING342108.amb3851.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAE52655; BAE52655; amb3851.
GeneID3803104.
KEGGmag:amb3851.
PATRIC22442008. VBIMagMag129836_3826.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAANDTQYR.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycMMAG342108:GJNU-3897-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_MAGSA
AccessionPrimary (citable) accession number: Q2W0H0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: January 10, 2006
Last modified: May 14, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways