Q2VZH9 (Q2VZH9_MAGSA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase HAMAP-Rule MF_01374
EC=3.1.2.6 HAMAP-Rule MF_01374

Alternative name(s):
Glyoxalase II HAMAP-Rule MF_01374

Gene names

Name:	gloB HAMAP-Rule MF_01374
Ordered Locus Names:	amb4192

Organism

Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) [Complete proteome] [HAMAP]

Taxonomic identifier

342108 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Magnetospirillum › Magnetospirillum magneticum

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP-Rule MF_01374 SAAS SAAS017782
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP-Rule MF_01374 SAAS SAAS017782
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP-Rule MF_01374 SAAS SAAS017782
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP-Rule MF_01374
Subunit structure	Monomer By similarity. HAMAP-Rule MF_01374 SAAS SAAS017782
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. HAMAP-Rule MF_01374

Ontologies

Keywords
Ligand	Metal-binding HAMAP-Rule MF_01374 SAAS SAAS017782 Zinc HAMAP-Rule MF_01374 SAAS SAAS017782
Molecular function	Hydrolase HAMAP-Rule MF_01374 SAAS SAAS017782 EMBL BAE52996.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutathione biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	70	1	Zinc 1 By similarity HAMAP-Rule MF_01374
	Metal binding	72	1	Zinc 1 By similarity HAMAP-Rule MF_01374
	Metal binding	74	1	Zinc 2 By similarity HAMAP-Rule MF_01374
	Metal binding	75	1	Zinc 2 By similarity HAMAP-Rule MF_01374
	Metal binding	128	1	Zinc 1 By similarity HAMAP-Rule MF_01374
	Metal binding	147	1	Zinc 1 By similarity HAMAP-Rule MF_01374
	Metal binding	147	1	Zinc 2 By similarity HAMAP-Rule MF_01374
	Metal binding	185	1	Zinc 2 By similarity HAMAP-Rule MF_01374

Sequences

	Sequence		Length	Mass (Da)
	Q2VZH9 [UniParc]. Last modified January 10, 2006. Version 1. Checksum: 09796E817846E834	FASTA	270	29,472
	10 20 30 40 50 60 MMLHSAYRCG WMSMAKIIVE QIPVLSDNYV YLAHEPLGGA TAAIDPAVSE PVLERLAARG 70 80 90 100 110 120 WTLTHILNTH HHGDHTGANL DLARRTGCAV VGAARDSERI PGITLEVSEG ETFMLGHAAV 130 140 150 160 170 180 TVLEVPGHTS GHVAYWLADS HVLFCGDTLF SLGCGRLFEG TAEEMWASLK KLRDLPPDTL 190 200 210 220 230 240 AYPAHEYTAG NGRFARLVER DNGALKARLD EVERLRAHNR PTVPVRLADE RAANPFLRAD 250 260 270 TAAIARAVGL EPGTDPALVF AELRRRKDVF

References

[1]

"Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1." Matsunaga T., Okamura Y., Fukuda Y., Wahyudi A.T., Murase Y., Takeyama H. DNA Res. 12:157-166(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AMB-1 / ATCC 700264.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP007255 Genomic DNA. Translation: BAE52996.1.
RefSeq	YP_423555.1. NC_007626.1.
3D structure databases
ProteinModelPortal	Q2VZH9.
ModBase	Search...
Protein-protein interaction databases
STRING	342108.amb4192.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAE52996; BAE52996; amb4192.
GeneID	3803857.
KEGG	mag:amb4192.
PATRIC	22442706. VBIMagMag129836_4171.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HOG000058041.
KO	K01069.
OMA	PFMRVHE.
Enzyme and pathway databases
BioCyc	MMAG342108:GJNU-4242-MONOMER.
UniPathway	UPA00619; UER00676.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2.
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
PIRSF	PIRSF005457. Glx. 1 hit.
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q2VZH9_MAGSA

Accession

Primary (citable) accession number: Q2VZH9

Entry history

Integrated into UniProtKB/TrEMBL:	January 10, 2006
Last sequence update:	January 10, 2006
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)