Reviewed, UniProtKB/Swiss-Prot Q2VRL0 (PLCZ1_CHICK)

Names and origin

Protein names

Recommended name:
    1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase zeta-1
    EC=3.1.4.11
Alternative name(s):
    Phosphoinositide phospholipase C-zeta-1
    Phospholipase C-zeta-1
      Short name=PLC-zeta-1

Gene names

Name:

PLCZ1

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. Ref.1 UniProtKB Q86YW0 UniProtKB Q8K4D7
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. UniProtKB P10688
Cofactor	Calcium By similarity. UniProtKB Q8K4D7
Subunit structure	Interacts via its C2 domain with PtdIns3P and, to a lesser extent, PtdIns5P in vitro By similarity. UniProtKB Q8K4D7
Subcellular location	Nucleus By similarity. Cytoplasm › perinuclear region By similarity. Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis By similarity. UniProtKB Q8K4D7
Tissue specificity	Expressed specifically in testis. Ref.1
Domain	The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity By similarity. UniProtKB Q8K4D7 The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization By similarity. UniProtKB Q8K4D7
Sequence similarities	Contains 1 C2 domain. Contains 1 EF-hand domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.

Ontologies

Keywords
Biological process	Fertilization Lipid degradation
Cellular component	Cytoplasm Nucleus
Ligand	Calcium
Molecular function	Developmental protein Hydrolase Transducer
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoinositide phospholipase C activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 637	637	1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase zeta-1		PRO_0000347249
Regions
	Domain	36 – 72	37	EF-hand
	Domain	157 – 302	146	PI-PLC X-box
	Domain	377 – 493	117	PI-PLC Y-box
	Domain	498 – 602	105	C2
Sites
	Active site	172	1	By similarity UniProtKB P10688
	Active site	217	1	By similarity UniProtKB P10688

Sequences

	Sequence		Length	Mass (Da)
	Q2VRL0-1 [UniParc]. Last modified January 10, 2006. Version 1. Checksum: 8FFFA824997BA401	FASTA	637	72,533
	10 20 30 40 50 60 MEENRWFLNI IQDGFMNGKI DFDSTVKLLE KLHMPFNLAH VKHVFKKTVD KRKIHTINIE 70 80 90 100 110 120 DFRAIYRAIV HRNEFHEIFC AYSENRKNLA DTELTAFLKK EQFKTEGAET TALEVILKYE 130 140 150 160 170 180 PIDEVRKRRQ LSFEGFIRYM SSEDCTIFKK EHRTVYQDMN HPLCDYFISS SHNTYLVSDQ 190 200 210 220 230 240 LIGPSDLNGY ISALLKGCRC LEIDCWDGSN NDPVVYHGHT LTSKITFCSV IHVVDKYAFA 250 260 270 280 290 300 ASDYPVVLSL ENHCSTKQQE RIAQYLLNIL GDKLLTSPIG DIEVTQLPSP EALKFKILVK 310 320 330 340 350 360 NKKCGTIEET MLRKGRDSHG ETGEVSEEEI TSSDEETDEK TPLYPKSGSS KRKSEGRSSP 370 380 390 400 410 420 PPRKKAKVKK MKIAMGLSDL VIYTKSEKFV SFEHSLAHQK CYENNSIGEL KAQKFVKHAA 430 440 450 460 470 480 NQFVSHTSRF ITRIYPKGTR AGSSNYNPQE FWNVGCQMVA LNFQTSGTPM ELQNGKFLDN 490 500 510 520 530 540 GGCGYILKPE FLRNRNSTFN PHNVGRYSNP LSLSIRLISG HQLPPSNLSK SNKADPLVQL 550 560 570 580 590 600 EIYGVPEDQA KRKSSVIKSN ALSPRWDETF SFTVQVPELA LIRFCVQDEI SLVANDFLGQ 610 620 630 YTLPLLSLSK GYCTVPLFSK SGGKLEPASL FVYVWYY

References

[1]

"Phospholipase Czeta, the trigger of egg activation in mammals, is present in a non-mammalian species." Coward K., Ponting C.P., Chang H.-Y., Hibbitt O., Savolainen P., Jones K.T., Parrington J. Reproduction 130:157-163(2005) [PubMed: 16049153] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY843531 mRNA. Translation: AAX48001.1.
IPI	IPI00589297.
RefSeq	NP_001034362.1.
UniGene	Gga.21947
3D structure databases
SMR	Q2VRL0. Positions 6-634, 61-635.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2VRL0.
Genome annotation databases
Ensembl	ENSGALT00000021418; ENSGALP00000021386; ENSGALG00000013123; Gallus gallus. [Genome view]
GeneID	418182.
KEGG	gga:418182.
Organism-specific databases
CTD	418182.
Phylogenomic databases
eggNOG	veNOG15439.
HOGENOM	HBG527074.
HOVERGEN	Q2VRL0.
InParanoid	Q2VRL0.
OMA	EIEETAR.
OrthoDB	EOG92VC1M.
PhylomeDB	Q2VRL0.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR011992. EF-hand-like_dom. IPR015359. Phospholipase_C_EF-hand-like. IPR001192. Phospholipase_C_Pinositol-sp_C. IPR001711. Phospholipase_C_Pinositol-sp_Y. IPR017946. PLC-like_Pdiesterase_TIM-brl. IPR000909. PLipase_C_PInositol-sp_X_dom. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit. G3DSA:3.20.20.190. PLC-like_Pdiesterase_TIM-brl. 1 hit.
Pfam	PF00168. C2. 1 hit. PF09279. efhand_like. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view]
PRINTS	PR00390. PHPHLIPASEC.
SMART	SM00239. C2. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
PROSITE	PS50004. C2. 1 hit. PS00018. EF_HAND_1. False negative. PS50222. EF_HAND_2. False negative. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLCZ1_CHICK

Accession

Primary (citable) accession number: Q2VRL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	January 10, 2006
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)