Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q2VRL0

- PLCZ1_CHICK

UniProt

Q2VRL0 - PLCZ1_CHICK

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

Gene

PLCZ1

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.By similarity1 PublicationCurated

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.By similarity

Cofactori

Calcium.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei172 – 1721By similarityPROSITE-ProRule annotation
Active sitei217 – 2171By similarityPROSITE-ProRule annotation

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. calcium ion transport Source: Ensembl
  2. egg activation Source: Ensembl
  3. intracellular signal transduction Source: InterPro
  4. lipid catabolic process Source: UniProtKB-KW
  5. multicellular organismal development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Transducer

Keywords - Biological processi

Fertilization, Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_198135. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-zeta-1
Phospholipase C-zeta-1By similarity
Short name:
PLC-zeta-1By similarity
Gene namesi
Name:PLCZ1By similarity
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Chromosome 1

Subcellular locationi

Nucleus By similarity. Cytoplasmperinuclear region By similarity
Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis (By similarity).By similarity

GO - Cellular componenti

  1. cytosol Source: InterPro
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6376371-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1PRO_0000347249Add
BLAST

Proteomic databases

PaxDbiQ2VRL0.

Expressioni

Tissue specificityi

Expressed specifically in testis.1 Publication

Interactioni

Subunit structurei

Interacts via its C2 domain with PtdIns3P and, to a lesser extent, PtdIns5P in vitro.By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000021386.

Structurei

3D structure databases

ProteinModelPortaliQ2VRL0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 7237EF-handSequence AnalysisAdd
BLAST
Domaini157 – 302146PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini377 – 493117PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini498 – 602105C2PROSITE-ProRule annotationAdd
BLAST

Domaini

The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity.By similarity
The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization.By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 EF-hand domain.Sequence Analysis
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00760000118936.
HOGENOMiHOG000006871.
HOVERGENiHBG053610.
InParanoidiQ2VRL0.
KOiK05861.
OMAiTHADINY.
OrthoDBiEOG7V49XT.
PhylomeDBiQ2VRL0.
TreeFamiTF313216.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028395. PLC-zeta1.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF29. PTHR10336:SF29. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2VRL0 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEENRWFLNI IQDGFMNGKI DFDSTVKLLE KLHMPFNLAH VKHVFKKTVD
60 70 80 90 100
KRKIHTINIE DFRAIYRAIV HRNEFHEIFC AYSENRKNLA DTELTAFLKK
110 120 130 140 150
EQFKTEGAET TALEVILKYE PIDEVRKRRQ LSFEGFIRYM SSEDCTIFKK
160 170 180 190 200
EHRTVYQDMN HPLCDYFISS SHNTYLVSDQ LIGPSDLNGY ISALLKGCRC
210 220 230 240 250
LEIDCWDGSN NDPVVYHGHT LTSKITFCSV IHVVDKYAFA ASDYPVVLSL
260 270 280 290 300
ENHCSTKQQE RIAQYLLNIL GDKLLTSPIG DIEVTQLPSP EALKFKILVK
310 320 330 340 350
NKKCGTIEET MLRKGRDSHG ETGEVSEEEI TSSDEETDEK TPLYPKSGSS
360 370 380 390 400
KRKSEGRSSP PPRKKAKVKK MKIAMGLSDL VIYTKSEKFV SFEHSLAHQK
410 420 430 440 450
CYENNSIGEL KAQKFVKHAA NQFVSHTSRF ITRIYPKGTR AGSSNYNPQE
460 470 480 490 500
FWNVGCQMVA LNFQTSGTPM ELQNGKFLDN GGCGYILKPE FLRNRNSTFN
510 520 530 540 550
PHNVGRYSNP LSLSIRLISG HQLPPSNLSK SNKADPLVQL EIYGVPEDQA
560 570 580 590 600
KRKSSVIKSN ALSPRWDETF SFTVQVPELA LIRFCVQDEI SLVANDFLGQ
610 620 630
YTLPLLSLSK GYCTVPLFSK SGGKLEPASL FVYVWYY
Length:637
Mass (Da):72,533
Last modified:January 10, 2006 - v1
Checksum:i8FFFA824997BA401
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY843531 mRNA. Translation: AAX48001.1.
RefSeqiNP_001034362.1. NM_001039273.1.
UniGeneiGga.21947.

Genome annotation databases

EnsembliENSGALT00000021418; ENSGALP00000021386; ENSGALG00000013123.
GeneIDi418182.
KEGGigga:418182.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY843531 mRNA. Translation: AAX48001.1 .
RefSeqi NP_001034362.1. NM_001039273.1.
UniGenei Gga.21947.

3D structure databases

ProteinModelPortali Q2VRL0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000021386.

Proteomic databases

PaxDbi Q2VRL0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSGALT00000021418 ; ENSGALP00000021386 ; ENSGALG00000013123 .
GeneIDi 418182.
KEGGi gga:418182.

Organism-specific databases

CTDi 89869.

Phylogenomic databases

eggNOGi NOG149692.
GeneTreei ENSGT00760000118936.
HOGENOMi HOG000006871.
HOVERGENi HBG053610.
InParanoidi Q2VRL0.
KOi K05861.
OMAi THADINY.
OrthoDBi EOG7V49XT.
PhylomeDBi Q2VRL0.
TreeFami TF313216.

Enzyme and pathway databases

Reactomei REACT_198135. Synthesis of IP3 and IP4 in the cytosol.

Miscellaneous databases

NextBioi 20821385.
PROi Q2VRL0.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028395. PLC-zeta1.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
PTHR10336:SF29. PTHR10336:SF29. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Phospholipase Czeta, the trigger of egg activation in mammals, is present in a non-mammalian species."
    Coward K., Ponting C.P., Chang H.-Y., Hibbitt O., Savolainen P., Jones K.T., Parrington J.
    Reproduction 130:157-163(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Testis1 Publication.

Entry informationi

Entry nameiPLCZ1_CHICK
AccessioniPrimary (citable) accession number: Q2VRL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: January 10, 2006
Last modified: October 29, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3