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Protein

Cytoplasmic tRNA 2-thiolation protein 2

Gene

CTU2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.UniRule annotation1 Publication

Pathwayi: 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis

This protein is involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis and in tRNA modification.

GO - Molecular functioni

GO - Biological processi

  • tRNA thio-modification Source: UniProtKB
  • tRNA wobble position uridine thiolation Source: GO_Central
  • tRNA wobble uridine modification Source: UniProtKB

Keywordsi

Biological processtRNA processing

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-20243
ReactomeiR-HSA-6782315 tRNA modification in the nucleus and cytosol
UniPathwayiUPA00988

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic tRNA 2-thiolation protein 2UniRule annotation
Alternative name(s):
Cytosolic thiouridylase subunit 2
Gene namesi
Name:CTU2UniRule annotation
Synonyms:C16orf84, NCS2UniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000174177.12
HGNCiHGNC:28005 CTU2
MIMi617057 gene
neXtProtiNX_Q2VPK5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000174177
PharmGKBiPA165449882

Polymorphism and mutation databases

BioMutaiCTU2
DMDMi121941955

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002891752 – 515Cytoplasmic tRNA 2-thiolation protein 2Add BLAST514

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylcysteineCombined sources1
Modified residuei415PhosphoserineCombined sources1
Modified residuei419PhosphoserineCombined sources1
Modified residuei435PhosphoserineCombined sources1
Modified residuei508PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ2VPK5
MaxQBiQ2VPK5
PaxDbiQ2VPK5
PeptideAtlasiQ2VPK5
PRIDEiQ2VPK5

PTM databases

iPTMnetiQ2VPK5
PhosphoSitePlusiQ2VPK5

Expressioni

Gene expression databases

BgeeiENSG00000174177
CleanExiHS_C16orf84
ExpressionAtlasiQ2VPK5 baseline and differential
GenevisibleiQ2VPK5 HS

Organism-specific databases

HPAiHPA041135
HPA041894

Interactioni

Subunit structurei

Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi131513, 34 interactors
DIPiDIP-48633N
IntActiQ2VPK5, 2 interactors
STRINGi9606.ENSP00000388320

Structurei

3D structure databases

ProteinModelPortaliQ2VPK5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CTU2/NCS2 family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2594 Eukaryota
ENOG410YGU3 LUCA
GeneTreeiENSGT00390000008797
HOGENOMiHOG000007287
HOVERGENiHBG107783
InParanoidiQ2VPK5
KOiK14169
PhylomeDBiQ2VPK5
TreeFamiTF313203

Family and domain databases

HAMAPiMF_03054 CTU2, 1 hit
InterProiView protein in InterPro
IPR019407 CTU2
PANTHERiPTHR20882 PTHR20882, 1 hit
PfamiView protein in Pfam
PF10288 CTU2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2VPK5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCQVGEDYGE PAPEEPPPAP RPSREQKCVK CKEAQPVVVI RAGDAFCRDC
60 70 80 90 100
FKAFYVHKFR AMLGKNRLIF PGEKVLLAWS GGPSSSSMVW QVLEGLSQDS
110 120 130 140 150
AKRLRFVAGV IFVDEGAACG QSLEERSKTL AEVKPILQAT GFPWHVVALE
160 170 180 190 200
EVFSLPPSVL WCSAQELVGS EGAYKAAVDS FLQQQHVLGA GGGPGPTQGE
210 220 230 240 250
EQPPQPPLDP QNLARPPAPA QTEALSQLFC SVRTLTAKEE LLQTLRTHLI
260 270 280 290 300
LHMARAHGYS KVMTGDSCTR LAIKLMTNLA LGRGAFLAWD TGFSDERHGD
310 320 330 340 350
VVVVRPMRDH TLKEVAFYNR LFSVPSVFTP AVDTKAPEKA SIHRLMEAFI
360 370 380 390 400
LRLQTQFPST VSTVYRTSEK LVKGPRDGPA AGDSGPRCLL CMCALDVDAA
410 420 430 440 450
DSATAFGAQT SSRLSQMQSP IPLTETRTPP GPCCSPGVGW AQRCGQGACR
460 470 480 490 500
REDPQACIEE QLCYSCRVNM KDLPSLDPLP PYILAEAQLR TQRAWGLQEI
510
RDCLIEDSDD EAGQS
Length:515
Mass (Da):56,107
Last modified:January 10, 2006 - v1
Checksum:i200CE7129FC89E65
GO
Isoform 2 (identifier: Q2VPK5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-87: Missing.

Note: No experimental confirmation available.
Show »
Length:428
Mass (Da):46,527
Checksum:iF964CA51F97BD822
GO
Isoform 3 (identifier: Q2VPK5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     474-515: PSLDPLPPYILAEAQLRTQRAWGLQEIRDCLIEDSDDEAGQS → GLGLAGDPGLSD

Note: Incomplete sequence.
Show »
Length:485
Mass (Da):52,467
Checksum:iF88DF9EE98405429
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti297R → W in AAH80540 (PubMed:15616553).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_062244186H → Y. Corresponds to variant dbSNP:rs2290895Ensembl.1
Natural variantiVAR_032595253M → V1 PublicationCorresponds to variant dbSNP:rs11549837Ensembl.1
Natural variantiVAR_032596332V → I. Corresponds to variant dbSNP:rs4782321Ensembl.1
Natural variantiVAR_032597416Q → R1 PublicationCorresponds to variant dbSNP:rs8059048Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0259501 – 87Missing in isoform 2. 1 PublicationAdd BLAST87
Alternative sequenceiVSP_039378474 – 515PSLDP…EAGQS → GLGLAGDPGLSD in isoform 3. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC138028 Genomic DNA No translation available.
BC021829 mRNA No translation available.
BC080540 mRNA Translation: AAH80540.1
BC108659 mRNA Translation: AAI08660.1
BC121805 mRNA Translation: AAI21806.1
BC125269 mRNA Translation: AAI25270.1
BC157881 mRNA Translation: AAI57882.1
CCDSiCCDS32506.1 [Q2VPK5-5]
CCDS45545.1 [Q2VPK5-1]
RefSeqiNP_001012777.1, NM_001012759.2 [Q2VPK5-1]
NP_001012780.1, NM_001012762.2 [Q2VPK5-5]
NP_001305436.1, NM_001318507.1
NP_001305442.1, NM_001318513.1 [Q2VPK5-3]
UniGeneiHs.592074

Genome annotation databases

EnsembliENST00000312060; ENSP00000308617; ENSG00000174177 [Q2VPK5-5]
ENST00000453996; ENSP00000388320; ENSG00000174177 [Q2VPK5-1]
GeneIDi348180
KEGGihsa:348180
UCSCiuc002flm.4 human [Q2VPK5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCTU2_HUMAN
AccessioniPrimary (citable) accession number: Q2VPK5
Secondary accession number(s): B2RXK0
, Q0P511, Q66K78, Q6P4C8, Q86SV4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: January 10, 2006
Last modified: May 23, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

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