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Protein

Scavenger receptor cysteine-rich type 1 protein M130

Gene

Cd163

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1 (By similarity).By similarity
After shedding, the soluble form (sCD163) may play an anti-inflammatory role.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Acute phase, Inflammatory response

Enzyme and pathway databases

ReactomeiR-MMU-2168880. Scavenging of heme from plasma.

Names & Taxonomyi

Protein namesi
Recommended name:
Scavenger receptor cysteine-rich type 1 protein M130
Alternative name(s):
CD_antigen: CD163
Cleaved into the following chain:
Soluble CD163
Short name:
sCD163
Gene namesi
Name:Cd163
Synonyms:M130
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2135946. Cd163.

Subcellular locationi

Soluble CD163 :
  • Secreted By similarity
  • Cell membrane By similarity; Single-pass type I membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini39 – 1045ExtracellularSequence analysisAdd BLAST1007
Transmembranei1046 – 1066HelicalSequence analysisAdd BLAST21
Topological domaini1067 – 1121CytoplasmicSequence analysisAdd BLAST55

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 38Sequence analysisAdd BLAST38
ChainiPRO_000023894039 – 1121Scavenger receptor cysteine-rich type 1 protein M130Add BLAST1083
ChainiPRO_000023894139 – ?Soluble CD163

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi75 ↔ 139PROSITE-ProRule annotation
Disulfide bondi88 ↔ 149PROSITE-ProRule annotation
Glycosylationi104N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi119 ↔ 129PROSITE-ProRule annotation
Glycosylationi138N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi183 ↔ 247PROSITE-ProRule annotation
Disulfide bondi196 ↔ 257PROSITE-ProRule annotation
Disulfide bondi227 ↔ 237PROSITE-ProRule annotation
Disulfide bondi290 ↔ 354PROSITE-ProRule annotation
Disulfide bondi303 ↔ 364PROSITE-ProRule annotation
Disulfide bondi334 ↔ 344PROSITE-ProRule annotation
Disulfide bondi397 ↔ 461PROSITE-ProRule annotation
Disulfide bondi410 ↔ 471PROSITE-ProRule annotation
Disulfide bondi441 ↔ 451PROSITE-ProRule annotation
Disulfide bondi502 ↔ 566PROSITE-ProRule annotation
Disulfide bondi515 ↔ 576PROSITE-ProRule annotation
Disulfide bondi546 ↔ 556PROSITE-ProRule annotation
Disulfide bondi607 ↔ 671PROSITE-ProRule annotation
Disulfide bondi620 ↔ 681PROSITE-ProRule annotation
Disulfide bondi651 ↔ 661PROSITE-ProRule annotation
Disulfide bondi744 ↔ 808PROSITE-ProRule annotation
Disulfide bondi757 ↔ 818PROSITE-ProRule annotation
Disulfide bondi788 ↔ 798PROSITE-ProRule annotation
Disulfide bondi864 ↔ 926PROSITE-ProRule annotation
Disulfide bondi895 ↔ 905PROSITE-ProRule annotation
Disulfide bondi955 ↔ 1019PROSITE-ProRule annotation
Disulfide bondi968 ↔ 1029PROSITE-ProRule annotation
Disulfide bondi999 ↔ 1009PROSITE-ProRule annotation

Post-translational modificationi

A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ2VLH6.
PaxDbiQ2VLH6.
PRIDEiQ2VLH6.

PTM databases

iPTMnetiQ2VLH6.
PhosphoSitePlusiQ2VLH6.
SwissPalmiQ2VLH6.

Expressioni

Tissue specificityi

Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen and mesenteric lymph nodes.1 Publication

Inductioni

Induced by antiinflammatory mediators such as glucocorticoids and IL10; suppressed by IL4.1 Publication

Gene expression databases

BgeeiENSMUSG00000008845.
CleanExiMM_CD163.
ExpressionAtlasiQ2VLH6. baseline and differential.
GenevisibleiQ2VLH6. MM.

Interactioni

Subunit structurei

Interacts with CSNK2B.By similarity

Protein-protein interaction databases

IntActiQ2VLH6. 1 interactor.
MINTiMINT-4124450.
STRINGi10090.ENSMUSP00000108160.

Structurei

3D structure databases

ProteinModelPortaliQ2VLH6.
SMRiQ2VLH6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 150SRCR 1PROSITE-ProRule annotationAdd BLAST101
Domaini157 – 258SRCR 2PROSITE-ProRule annotationAdd BLAST102
Domaini265 – 365SRCR 3PROSITE-ProRule annotationAdd BLAST101
Domaini372 – 472SRCR 4PROSITE-ProRule annotationAdd BLAST101
Domaini477 – 577SRCR 5PROSITE-ProRule annotationAdd BLAST101
Domaini582 – 682SRCR 6PROSITE-ProRule annotationAdd BLAST101
Domaini719 – 819SRCR 7PROSITE-ProRule annotationAdd BLAST101
Domaini824 – 927SRCR 8PROSITE-ProRule annotationAdd BLAST104
Domaini930 – 1030SRCR 9PROSITE-ProRule annotationAdd BLAST101

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1091 – 1094Internalization signal4

Domaini

The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.By similarity

Sequence similaritiesi

Contains 9 SRCR domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHBC. Eukaryota.
ENOG410XQVR. LUCA.
GeneTreeiENSGT00840000129699.
HOGENOMiHOG000290652.
HOVERGENiHBG080943.
InParanoidiQ2VLH6.
KOiK06545.
OMAiKLIGKVC.
TreeFamiTF329295.

Family and domain databases

Gene3Di3.10.250.10. 9 hits.
InterProiIPR001190. SRCR.
IPR017448. SRCR-like_dom.
[Graphical view]
PfamiPF00530. SRCR. 9 hits.
[Graphical view]
PRINTSiPR00258. SPERACTRCPTR.
SMARTiSM00202. SR. 9 hits.
[Graphical view]
SUPFAMiSSF56487. SSF56487. 9 hits.
PROSITEiPS00420. SRCR_1. 3 hits.
PS50287. SRCR_2. 9 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2VLH6-1) [UniParc]FASTAAdd to basket
Also known as: CD163v2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGGHRMVLLG GAGSPGCKRF VHLGFFVVAV SSLLSASAVT NAPGEMKKEL
60 70 80 90 100
RLAGGENNCS GRVELKIHDK WGTVCSNGWS MNEVSVVCQQ LGCPTSIKAL
110 120 130 140 150
GWANSSAGSG YIWMDKVSCT GNESALWDCK HDGWGKHNCT HEKDAGVTCS
160 170 180 190 200
DGSNLEMRLV NSAGHRCLGR VEIKFQGKWG TVCDDNFSKD HASVICKQLG
210 220 230 240 250
CGSAISFSGS AKLGAGSGPI WLDDLACNGN ESALWDCKHR GWGKHNCDHA
260 270 280 290 300
EDVGVICLEG ADLSLRLVDG VSRCSGRLEV RFQGEWGTVC DDNWDLRDAS
310 320 330 340 350
VVCKQLGCPT AISAIGRVNA SEGSGQIWLD NISCEGHEAT LWECKHQEWG
360 370 380 390 400
KHYCHHREDA GVTCSDGADL ELRLVGGGSR CAGIVEVEIQ KLTGKMCSRG
410 420 430 440 450
WTLADADVVC RQLGCGSALQ TQAKIYSKTG ATNTWLFPGS CNGNETTFWQ
460 470 480 490 500
CKNWQWGGLS CDNFEEAKVT CSGHREPRLV GGEIPCSGRV EVKHGDVWGS
510 520 530 540 550
VCDFDLSLEA ASVVCRELQC GTVVSILGGA HFGEGSGQIW GEEFQCSGDE
560 570 580 590 600
SHLSLCSVAP PLDRTCTHSR DVSVVCSRYI DIRLAGGESS CEGRVELKTL
610 620 630 640 650
GAWGPLCSSH WDMEDAHVLC QQLKCGVAQS IPEGAHFGKG AGQVWSHMFH
660 670 680 690 700
CTGTEEHIGD CLMTALGAPT CSEGQVASVI CSGNQSQTLL PCSSLSPVQT
710 720 730 740 750
TSSTIPKESE VPCIASGQLR LVGGGGRCAG RVEVYHEGSW GTVCDDNWDM
760 770 780 790 800
TDANVVCKQL DCGVAINATG SAYFGEGAGA IWLDEVICTG KESHIWQCHS
810 820 830 840 850
HGWGRHNCRH KEDAGVICSE FMSLRLTNEA HKENCTGRLE VFYNGTWGSI
860 870 880 890 900
GSSNMSPTTV GVVCRQLGCA DNGTVKPIPS DKTPSRPMWV DRVQCPKGVD
910 920 930 940 950
TLWQCPSSPW KQRQASPSSQ ESWIICDNKI RLQEGHTDCS GRVEIWHKGS
960 970 980 990 1000
WGTVCDDSWD LNDAKVVCKQ LGCGQAVKAL KEAAFGPGTG PIWLNEIKCR
1010 1020 1030 1040 1050
GNESSLWDCP AKPWSHSDCG HKEDASIQCL PKMTSESHHG TGHPTLTALL
1060 1070 1080 1090 1100
VCGAILLVLL IVFLLWTLKR RQIQRLTVSS RGEVLIHQVQ YQEMDSKADD
1110 1120
LDLLKSSGVI QRHTEKENDN L
Length:1,121
Mass (Da):120,921
Last modified:July 27, 2011 - v2
Checksum:i553B1EF5ED5064BD
GO
Isoform 2 (identifier: Q2VLH6-2) [UniParc]FASTAAdd to basket
Also known as: CD163v3

The sequence of this isoform differs from the canonical sequence as follows:
     1108-1121: GVIQRHTEKENDNL → ENSNNSYDFNDDGLTSLSKYLPISGIKKGSFRGTLRRKMIIYNPLRLEFKKP

Show »
Length:1,159
Mass (Da):125,292
Checksum:iF7FFA963E4277CC1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti492V → M in AAY99764 (Ref. 2) Curated1
Sequence conflicti735Y → C in AAK16065 (PubMed:11345593).Curated1
Sequence conflicti834N → S in AAY99763 (Ref. 2) Curated1
Sequence conflicti950S → F in AAY99763 (Ref. 2) Curated1
Sequence conflicti1072Q → R in AAK16065 (PubMed:11345593).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0190161108 – 1121GVIQR…ENDNL → ENSNNSYDFNDDGLTSLSKY LPISGIKKGSFRGTLRRKMI IYNPLRLEFKKP in isoform 2. 1 PublicationAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF274883 mRNA. Translation: AAK16065.1.
DQ058616 mRNA. Translation: AAY99763.1.
DQ058617 mRNA. Translation: AAY99764.1.
CH466523 Genomic DNA. Translation: EDK99729.1.
BC145793 mRNA. Translation: AAI45794.1.
CCDSiCCDS20516.1. [Q2VLH6-1]
CCDS51905.1. [Q2VLH6-2]
RefSeqiNP_444324.2. NM_053094.2. [Q2VLH6-1]
UniGeneiMm.37426.

Genome annotation databases

EnsembliENSMUST00000032234; ENSMUSP00000032234; ENSMUSG00000008845. [Q2VLH6-1]
ENSMUST00000112541; ENSMUSP00000108160; ENSMUSG00000008845. [Q2VLH6-2]
GeneIDi93671.
KEGGimmu:93671.
UCSCiuc009dqn.2. mouse. [Q2VLH6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF274883 mRNA. Translation: AAK16065.1.
DQ058616 mRNA. Translation: AAY99763.1.
DQ058617 mRNA. Translation: AAY99764.1.
CH466523 Genomic DNA. Translation: EDK99729.1.
BC145793 mRNA. Translation: AAI45794.1.
CCDSiCCDS20516.1. [Q2VLH6-1]
CCDS51905.1. [Q2VLH6-2]
RefSeqiNP_444324.2. NM_053094.2. [Q2VLH6-1]
UniGeneiMm.37426.

3D structure databases

ProteinModelPortaliQ2VLH6.
SMRiQ2VLH6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ2VLH6. 1 interactor.
MINTiMINT-4124450.
STRINGi10090.ENSMUSP00000108160.

PTM databases

iPTMnetiQ2VLH6.
PhosphoSitePlusiQ2VLH6.
SwissPalmiQ2VLH6.

Proteomic databases

MaxQBiQ2VLH6.
PaxDbiQ2VLH6.
PRIDEiQ2VLH6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032234; ENSMUSP00000032234; ENSMUSG00000008845. [Q2VLH6-1]
ENSMUST00000112541; ENSMUSP00000108160; ENSMUSG00000008845. [Q2VLH6-2]
GeneIDi93671.
KEGGimmu:93671.
UCSCiuc009dqn.2. mouse. [Q2VLH6-1]

Organism-specific databases

CTDi9332.
MGIiMGI:2135946. Cd163.

Phylogenomic databases

eggNOGiENOG410IHBC. Eukaryota.
ENOG410XQVR. LUCA.
GeneTreeiENSGT00840000129699.
HOGENOMiHOG000290652.
HOVERGENiHBG080943.
InParanoidiQ2VLH6.
KOiK06545.
OMAiKLIGKVC.
TreeFamiTF329295.

Enzyme and pathway databases

ReactomeiR-MMU-2168880. Scavenging of heme from plasma.

Miscellaneous databases

PROiQ2VLH6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000008845.
CleanExiMM_CD163.
ExpressionAtlasiQ2VLH6. baseline and differential.
GenevisibleiQ2VLH6. MM.

Family and domain databases

Gene3Di3.10.250.10. 9 hits.
InterProiIPR001190. SRCR.
IPR017448. SRCR-like_dom.
[Graphical view]
PfamiPF00530. SRCR. 9 hits.
[Graphical view]
PRINTSiPR00258. SPERACTRCPTR.
SMARTiSM00202. SR. 9 hits.
[Graphical view]
SUPFAMiSSF56487. SSF56487. 9 hits.
PROSITEiPS00420. SRCR_1. 3 hits.
PS50287. SRCR_2. 9 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC163A_MOUSE
AccessioniPrimary (citable) accession number: Q2VLH6
Secondary accession number(s): A6H691, Q2VLH5, Q99MX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.