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Protein

Scavenger receptor cysteine-rich type 1 protein M130

Gene

CD163

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1 (By similarity). May play a role in the process of infection of porcine monocytes/macrophages by African swine fever virus (ASFV). In case of porcine reproductive and respiratory syndrome virus (PRRSV), serves mediates virion attachment and plays a role in viral entry.By similarity1 Publication
After shedding, the soluble form (sCD163) may play an anti-inflammatory role.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Host cell receptor for virus entry, Receptor

Keywords - Biological processi

Acute phase, Host-virus interaction, Inflammatory response

Names & Taxonomyi

Protein namesi
Recommended name:
Scavenger receptor cysteine-rich type 1 protein M130
Alternative name(s):
CD_antigen: CD163
Cleaved into the following chain:
Soluble CD163
Short name:
sCD163
Gene namesi
Name:CD163
Synonyms:M130
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Soluble CD163 :
  • Secreted By similarity
  • Cell membrane By similarity; Single-pass type I membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini47 – 1044998ExtracellularSequence analysisAdd
BLAST
Transmembranei1045 – 106521HelicalSequence analysisAdd
BLAST
Topological domaini1066 – 111550CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4646Sequence analysisAdd
BLAST
Chaini47 – 11151069Scavenger receptor cysteine-rich type 1 protein M130PRO_0000238942Add
BLAST
Chaini47 – ?Soluble CD163PRO_0000238943

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi76 ↔ 140PROSITE-ProRule annotation
Disulfide bondi89 ↔ 150PROSITE-ProRule annotation
Glycosylationi105 – 1051N-linked (GlcNAc...)Sequence analysis
Disulfide bondi120 ↔ 130PROSITE-ProRule annotation
Glycosylationi139 – 1391N-linked (GlcNAc...)Sequence analysis
Disulfide bondi183 ↔ 247PROSITE-ProRule annotation
Disulfide bondi196 ↔ 257PROSITE-ProRule annotation
Disulfide bondi227 ↔ 237PROSITE-ProRule annotation
Disulfide bondi290 ↔ 354PROSITE-ProRule annotation
Disulfide bondi303 ↔ 364PROSITE-ProRule annotation
Disulfide bondi334 ↔ 344PROSITE-ProRule annotation
Disulfide bondi397 ↔ 461PROSITE-ProRule annotation
Disulfide bondi410 ↔ 471PROSITE-ProRule annotation
Disulfide bondi441 ↔ 451PROSITE-ProRule annotation
Disulfide bondi502 ↔ 566PROSITE-ProRule annotation
Disulfide bondi515 ↔ 576PROSITE-ProRule annotation
Disulfide bondi546 ↔ 556PROSITE-ProRule annotation
Disulfide bondi607 ↔ 671PROSITE-ProRule annotation
Disulfide bondi620 ↔ 681PROSITE-ProRule annotation
Disulfide bondi651 ↔ 661PROSITE-ProRule annotation
Disulfide bondi743 ↔ 807PROSITE-ProRule annotation
Disulfide bondi756 ↔ 817PROSITE-ProRule annotation
Disulfide bondi787 ↔ 797PROSITE-ProRule annotation
Disulfide bondi863 ↔ 924PROSITE-ProRule annotation
Disulfide bondi894 ↔ 904PROSITE-ProRule annotation
Glycosylationi936 – 9361N-linked (GlcNAc...)Sequence analysis
Disulfide bondi953 ↔ 1017PROSITE-ProRule annotation
Disulfide bondi966 ↔ 1027PROSITE-ProRule annotation
Disulfide bondi997 ↔ 1007PROSITE-ProRule annotation

Post-translational modificationi

A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs (By similarity).By similarity
Phosphorylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PeptideAtlasiQ2VL90.

Expressioni

Tissue specificityi

Expressed in monocytes and macrophages. Detected only in one population of monocytes (CD163+) which is in advanced maturation stage.1 Publication

Inductioni

Suppressed when monocytes are differentiated towards dendritic cells by CSF1 and IL4.1 Publication

Interactioni

Subunit structurei

Interacts with CSNK2B.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ2VL90.
SMRiQ2VL90. Positions 715-821, 926-1030.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 151101SRCR 1PROSITE-ProRule annotationAdd
BLAST
Domaini158 – 258101SRCR 2PROSITE-ProRule annotationAdd
BLAST
Domaini265 – 365101SRCR 3PROSITE-ProRule annotationAdd
BLAST
Domaini372 – 472101SRCR 4PROSITE-ProRule annotationAdd
BLAST
Domaini477 – 577101SRCR 5PROSITE-ProRule annotationAdd
BLAST
Domaini582 – 682101SRCR 6PROSITE-ProRule annotationAdd
BLAST
Domaini718 – 818101SRCR 7PROSITE-ProRule annotationAdd
BLAST
Domaini823 – 925103SRCR 8PROSITE-ProRule annotationAdd
BLAST
Domaini928 – 1028101SRCR 9PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1090 – 10934Internalization signal

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi694 – 6974Poly-Ser

Domaini

The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.By similarity

Sequence similaritiesi

Contains 9 SRCR domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG080943.
InParanoidiQ2VL90.
KOiK06545.

Family and domain databases

Gene3Di3.10.250.10. 9 hits.
InterProiIPR001190. SRCR.
IPR017448. SRCR-like_dom.
[Graphical view]
PfamiPF00530. SRCR. 9 hits.
[Graphical view]
PRINTSiPR00258. SPERACTRCPTR.
SMARTiSM00202. SR. 9 hits.
[Graphical view]
SUPFAMiSSF56487. SSF56487. 9 hits.
PROSITEiPS00420. SRCR_1. 3 hits.
PS50287. SRCR_2. 9 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2VL90-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDKLRMVLHE NSGSADFRRC SAHLSSFTFA VVAVLSACLV TSSLGGKDKE
60 70 80 90 100
LRLTGGENKC SGRVEVKVQE EWGTVCNNGW DMDVVSVVCR QLGCPTAIKA
110 120 130 140 150
TGWANFSAGS GRIWMDHVSC RGNESALWDC KHDGWGKHNC THQQDAGVTC
160 170 180 190 200
SDGSDLEMGL VNGGNRCLGR IEVKFQGRWG TVCDDNFNIN HASVVCKQLE
210 220 230 240 250
CGSAVSFSGS ANFGEGSGPI WFDDLVCNGN ESALWNCKHE GWGKHNCDHA
260 270 280 290 300
EDAGVICLNG ADLKLRVVDG VTECSGRLEV KFQGEWGTIC DDGWDSDDAA
310 320 330 340 350
VACKQLGCPT AVTAIGRVNA SEGTGHIWLD SVSCHGHESA LWQCRHHEWG
360 370 380 390 400
KHYCNHDEDA GVTCSDGSDL ELRLKGGGSH CAGTVEVEIQ KLVGKVCDRS
410 420 430 440 450
WGLKEADVVC RQLGCGSALK TSYQVYSKTK ATNTWLFVSS CNGNETSLWD
460 470 480 490 500
CKNWQWGGLS CDHYDEAKIT CSAHRKPRLV GGDIPCSGRV EVQHGDTWGT
510 520 530 540 550
VCDSDFSLEA ASVLCRELQC GTVVSLLGGA HFGEGSGQIW AEEFQCEGHE
560 570 580 590 600
SHLSLCPVAP RPDGTCSHSR DVGVVCSRYT QIRLVNGKTP CEGRVELNIL
610 620 630 640 650
GSWGSLCNSH WDMEDAHVLC QQLKCGVALS IPGGAPFGKG SEQVWRHMFH
660 670 680 690 700
CTGTEKHMGD CSVTALGASL CSSGQVASVI CSGNQSQTLS PCNSSSSDPS
710 720 730 740 750
SSIISEENGV ACIGSGQLRL VDGGGRCAGR VEVYHEGSWG TICDDSWDLN
760 770 780 790 800
DAHVVCKQLS CGWAINATGS AHFGEGTGPI WLDEINCNGK ESHIWQCHSH
810 820 830 840 850
GWGRHNCRHK EDAGVICSEF MSLRLISENS RETCAGRLEV FYNGAWGSVG
860 870 880 890 900
RNSMSPATVG VVCRQLGCAD RGDISPASSD KTVSRHMWVD NVQCPKGPDT
910 920 930 940 950
LWQCPSSPWK KRLASPSEET WITCANKIRL QEGNTNCSGR VEIWYGGSWG
960 970 980 990 1000
TVCDDSWDLE DAQVVCRQLG CGSALEAGKE AAFGQGTGPI WLNEVKCKGN
1010 1020 1030 1040 1050
ETSLWDCPAR SWGHSDCGHK EDAAVTCSEI AKSRESLHAT GRSSFVALAI
1060 1070 1080 1090 1100
FGVILLACLI AFLIWTQKRR QRQRLSVFSG GENSVHQIQY REMNSCLKAD
1110
ETDMLNPSGD HSEVQ
Length:1,115
Mass (Da):120,456
Last modified:January 10, 2006 - v1
Checksum:i68877E34BA5955C1
GO
Isoform 2 (identifier: Q2VL90-2) [UniParc]FASTAAdd to basket

Also known as: CD163v3

The sequence of this isoform differs from the canonical sequence as follows:
     15-260: Missing.

Show »
Length:869
Mass (Da):94,061
Checksum:i6C3A917D59603DAA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 113HEN → LED in CAC84397 (Ref. 2) Curated
Sequence conflicti159 – 1591G → R in CAC84397 (Ref. 2) Curated
Sequence conflicti177 – 1771G → E in CAC84397 (Ref. 2) Curated
Sequence conflicti271 – 2711V → L in CAC84397 (Ref. 2) Curated
Sequence conflicti357 – 3571D → N in AAZ50617 (Ref. 1) Curated
Sequence conflicti357 – 3571D → N in CAC84397 (Ref. 2) Curated
Sequence conflicti708 – 7081N → S in CAC84397 (Ref. 2) Curated
Sequence conflicti735 – 7373HEG → PGA in CAC84397 (Ref. 2) Curated
Sequence conflicti851 – 8511R → K in AAZ50617 (Ref. 1) Curated
Sequence conflicti981 – 9811A → P in CAC84397 (Ref. 2) Curated
Sequence conflicti1002 – 10021T → P in CAC84397 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei15 – 260246Missing in isoform 2. 1 PublicationVSP_019017Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ067278 mRNA. Translation: AAZ50616.1.
DQ067279 mRNA. Translation: AAZ50617.1.
AJ311716 mRNA. Translation: CAC84397.1.
RefSeqiNP_999141.1. NM_213976.1.
UniGeneiSsc.100404.
Ssc.5053.

Genome annotation databases

GeneIDi397031.
KEGGissc:397031.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ067278 mRNA. Translation: AAZ50616.1.
DQ067279 mRNA. Translation: AAZ50617.1.
AJ311716 mRNA. Translation: CAC84397.1.
RefSeqiNP_999141.1. NM_213976.1.
UniGeneiSsc.100404.
Ssc.5053.

3D structure databases

ProteinModelPortaliQ2VL90.
SMRiQ2VL90. Positions 715-821, 926-1030.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PeptideAtlasiQ2VL90.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397031.
KEGGissc:397031.

Organism-specific databases

CTDi9332.

Phylogenomic databases

HOVERGENiHBG080943.
InParanoidiQ2VL90.
KOiK06545.

Family and domain databases

Gene3Di3.10.250.10. 9 hits.
InterProiIPR001190. SRCR.
IPR017448. SRCR-like_dom.
[Graphical view]
PfamiPF00530. SRCR. 9 hits.
[Graphical view]
PRINTSiPR00258. SPERACTRCPTR.
SMARTiSM00202. SR. 9 hits.
[Graphical view]
SUPFAMiSSF56487. SSF56487. 9 hits.
PROSITEiPS00420. SRCR_1. 3 hits.
PS50287. SRCR_2. 9 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Scavenger receptor cd163 is a cell permissive factor for infection with porcine reproductive and respiratory syndrome viruses."
    Welch S.-K.W., Calvert J.G., Slade D.E., Shields S.L.
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
  2. "Cloning and characterization of the cDNA coding for the porcine homologous to human CD163 antigen."
    Gomez N., Ortuno E., Alonso F., Dominguez J., Ezquerra A.
    Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-1115 (ISOFORM 1).
    Tissue: Lung.
  3. "Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection."
    Sanchez-Torres C., Gomez-Puertas P., Gomez-del-Moral M., Alonso F., Escribano J.M., Ezquerra A., Dominguez J.
    Arch. Virol. 148:2307-2323(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  4. "In vitro differentiation of porcine blood CD163- and CD163+ monocytes into functional dendritic cells."
    Chamorro S., Revilla C., Gomez N., Alvarez B., Alonso F., Ezquerra A., Dominguez J.
    Immunobiology 209:57-65(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. "The minor envelope glycoproteins GP2a and GP4 of porcine reproductive and respiratory syndrome virus interact with the receptor CD163."
    Das P.B., Dinh P.X., Ansari I.H., de Lima M., Osorio F.A., Pattnaik A.K.
    J. Virol. 84:1731-1740(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS GLYCOPROTEIN 4 AND GLYCOPROTEIN 2A.

Entry informationi

Entry nameiC163A_PIG
AccessioniPrimary (citable) accession number: Q2VL90
Secondary accession number(s): Q2VL89, Q8HY22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: January 10, 2006
Last modified: July 6, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-6 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.