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Q2V6K0

- UFOG6_FRAAN

UniProt

Q2V6K0 - UFOG6_FRAAN

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Protein
UDP-glucose flavonoid 3-O-glucosyltransferase 6
Gene
GT6
Organism
Fragaria ananassa (Strawberry)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Broad spectrum multifunctional glucosyltransferase. Catalyzes the formation of flavonol 3-O-glucosides during fruit ripening. Accepted substrates include several flavonoids, hydroxycoumarins and beta-naphthols. Uses UDP-Glc as a sugar donor, but not UDP-Gal or UDP-GlcUA. May also be involved in detoxification of xenobiotics.1 Publication

Catalytic activityi

UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.1 Publication

Kineticsi

The kinetic constants are determined for the recombinant GST-fusion protein.1 Publication

  1. KM=5.3 µM for 3-hydroxyflavone1 Publication
  2. KM=2.0 mM for UDP-glucose1 Publication

Vmax=1.4 nmol/sec/mg enzyme with 3-hydroxyflavone as substrate

Vmax=0.5 nmol/sec/mg enzyme with UDP-glucose as substrate1 Publication

pH dependencei

Optimum pH is 7.5-8.1 Publication

Temperature dependencei

Optimum temperature is 35 degrees Celsius.1 Publication

GO - Molecular functioni

  1. flavonol 3-O-glucosyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17433.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucose flavonoid 3-O-glucosyltransferase 6 (EC:2.4.1.91)
Alternative name(s):
Flavonol 3-O-glucosyltransferase 6
Short name:
FaGT6
Gene namesi
Name:GT6
OrganismiFragaria ananassa (Strawberry)
Taxonomic identifieri3747 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeRosoideaePotentilleaeFragariinaeFragaria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479UDP-glucose flavonoid 3-O-glucosyltransferase 6
PRO_0000413769Add
BLAST

Expressioni

Tissue specificityi

Strongly expressed in achenes, with lower expression levels detected in receptacles.1 Publication

Developmental stagei

The expression in receptacles is ripening-related, with highest expression detected in red fruit.1 Publication

Inductioni

By de-achening. By injection with salicylic acid, with transcript levels increasing by a factor of 5-6 at 4 hours post-injection, remaining stable until 6 hours post-injection and falling below control levels at 8 hours post-injection. Down-regulated by synthetic auxin naphthaleneacetic acid (NAA).1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ2V6K0.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2V6K0-1 [UniParc]FASTAAdd to Basket

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MKKASELIFI PIPGIGHIVS TVEIAKLLLC RDDNLFITIL IMKFPFTADG    50
SDVYIKSLAV DPSLKTQRIR FVNLPQEHFQ GTGATGFFTF IDSHKSHVKD 100
AVTRLMETKS ETTRIAGFVI DMFCTGMIDL ANEFGLPSYV FYTSGAADLG 150
LMFHLQALRD EENKDCTEFK DSDAELVVSS FVNPLPAARV LPSVVFEKEG 200
GNFFLNFAKR YRETKGILVN TFLELEPHAI QSLSSDGKIL PVYPVGPILN 250
VKSEGNQVSS EKSKQKSDIL EWLDDQPPSS VVFLCFGSMG CFGEDQVKEI 300
AHALEQGGIR FLWSLRQPSK EKIGFPSDYT DYKAVLPEGF LDRTTDLGKV 350
IGWAPQLAIL AHPAVGGFVS HCGWNSTLES IWYGVPIATW PFYAEQQVNA 400
FELVKELKLA VEIDMGYRKD SGVIVSRENI EKGIKEVMEQ ESELRKRVKE 450
MSQMSRKALE EDGSSYSSLG RFLDQIQTS 479
Length:479
Mass (Da):53,477
Last modified:January 10, 2006 - v1
Checksum:iF7E4BAD1CB8D14D4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ289587 mRNA. Translation: ABB92748.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ289587 mRNA. Translation: ABB92748.1 .

3D structure databases

ProteinModelPortali Q2V6K0.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT1. Glycosyltransferase Family 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-17433.

Family and domain databases

InterProi IPR002213. UDP_glucos_trans.
[Graphical view ]
PANTHERi PTHR11926. PTHR11926. 1 hit.
Pfami PF00201. UDPGT. 1 hit.
[Graphical view ]
PROSITEi PS00375. UDPGT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Multi-substrate flavonol O-glucosyltransferases from strawberry (Fragaria x ananassa) achene and receptacle."
    Griesser M., Vitzthum F., Fink B., Bellido M.L., Raasch C., Munoz-Blanco J., Schwab W.
    J. Exp. Bot. 59:2611-2625(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    Strain: cv. Elsanta.
    Tissue: Fruit.

Entry informationi

Entry nameiUFOG6_FRAAN
AccessioniPrimary (citable) accession number: Q2V6K0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: January 10, 2006
Last modified: February 19, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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