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Q2V6J9

- UFOG7_FRAAN

UniProt

Q2V6J9 - UFOG7_FRAAN

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Protein

UDP-glucose flavonoid 3-O-glucosyltransferase 7

Gene

GT7

Organism
Fragaria ananassa (Strawberry)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Broad spectrum multifunctional glucosyltransferase. Catalyzes the formation of flavonol 3-O- and 4'-O-glucosides during fruit ripening. Accepted substrates include several flavonoids, hydroxycoumarins and beta-naphthols. Uses UDP-Glc as a sugar donor, but not UDP-Gal or UDP-GlcUA. May also be involved in detoxification of xenobiotics.1 Publication

Catalytic activityi

UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.1 Publication

Kineticsi

The kinetic constants are determined for the recombinant GST-fusion protein.1 Publication

  1. KM=3.5 µM for isorhamnetin1 Publication
  2. KM=0.6 mM for UDP-glucose1 Publication

Vmax=2.8 nmol/sec/mg enzyme with isorhamnetin as substrate1 Publication

Vmax=2.7 nmol/sec/mg enzyme with UDP-glucose as substrate1 Publication

pH dependencei

Optimum pH is 6.5.1 Publication

Temperature dependencei

Optimum temperature is 45 degrees Celsius.1 Publication

GO - Molecular functioni

  1. flavonol 3-O-glucosyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucose flavonoid 3-O-glucosyltransferase 71 Publication (EC:2.4.1.911 Publication)
Alternative name(s):
Flavonol 3-O-glucosyltransferase 71 Publication
Short name:
FaGT71 Publication
Gene namesi
Name:GT7Imported
OrganismiFragaria ananassa (Strawberry)
Taxonomic identifieri3747 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeRosoideaePotentilleaeFragariinaeFragaria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 487487UDP-glucose flavonoid 3-O-glucosyltransferase 7PRO_0000413770Add
BLAST

Expressioni

Tissue specificityi

Strongly expressed in achenes and receptacles.1 Publication

Developmental stagei

The expression in receptacles is ripening-related, with highest expression detected in red fruit.1 Publication

Inductioni

By de-achening. By injection with salicylic acid, with transcript levels increasing by a factor of 5-6 at 4 hours post-injection, remaining stable until 6 hours post-injection and falling below control levels at 8 hours post-injection. Down-regulated by synthetic auxin naphthaleneacetic acid (NAA).1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Sequence Analysis

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2V6J9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAMETKSCQQ LHIFFLPFMA RGHSIPLTDI AKLFSSHGAR CTIVTTPLNA
60 70 80 90 100
PLFSKATQRG EIELVLIKFP SAEAGLPQDC ESADLITTQD MLGKFVKATF
110 120 130 140 150
LIEPHFEKIL DEHRPHCLVA DAFFTWATDV AAKFRIPRLY FHGTGFFALC
160 170 180 190 200
ASLSVMMYQP HSNLSSDSES FVIPNLPDEI KMTRSQLPVF PDESEFMKML
210 220 230 240 250
KASIEIEERS YGVIVNSFYE LEPAYANHYR KVFGRKAWHI GPVSFCNKAI
260 270 280 290 300
EDKAERGSIK SSTAEKHECL KWLDSKKPRS VVYVSFGSMV RFADSQLLEI
310 320 330 340 350
ATGLEASGQD FIWVVKKEKK EVEEWLPEGF EKRMEGKGLI IRDWAPQVLI
360 370 380 390 400
LEHEAIGAFV THCGWNSILE AVSAGVPMIT WPVFGEQFYN EKLVTEIHRI
410 420 430 440 450
GVPVGSEKWA LSFVDVNAET EGRVRREAIE EAVTRIMVGD EAVETRSRVK
460 470 480
ELGENARRAV EEGGSSFLDL SALVGELNDL AFGGLVE
Length:487
Mass (Da):54,652
Last modified:January 10, 2006 - v1
Checksum:i313E7EB3889985FD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ289588 mRNA. Translation: ABB92749.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ289588 mRNA. Translation: ABB92749.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT1. Glycosyltransferase Family 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002213. UDP_glucos_trans.
[Graphical view ]
PANTHERi PTHR11926. PTHR11926. 1 hit.
Pfami PF00201. UDPGT. 1 hit.
[Graphical view ]
PROSITEi PS00375. UDPGT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Multi-substrate flavonol O-glucosyltransferases from strawberry (Fragaria x ananassa) achene and receptacle."
    Griesser M., Vitzthum F., Fink B., Bellido M.L., Raasch C., Munoz-Blanco J., Schwab W.
    J. Exp. Bot. 59:2611-2625(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    Strain: cv. Elsanta1 Publication.
    Tissue: Fruit1 Publication.

Entry informationi

Entry nameiUFOG7_FRAAN
AccessioniPrimary (citable) accession number: Q2V6J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: January 10, 2006
Last modified: October 1, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3