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Q2V6J9

- UFOG7_FRAAN

UniProt

Q2V6J9 - UFOG7_FRAAN

Protein

UDP-glucose flavonoid 3-O-glucosyltransferase 7

Gene

GT7

Organism
Fragaria ananassa (Strawberry)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 28 (01 Oct 2014)
      Sequence version 1 (10 Jan 2006)
      Previous versions | rss
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    Functioni

    Broad spectrum multifunctional glucosyltransferase. Catalyzes the formation of flavonol 3-O- and 4'-O-glucosides during fruit ripening. Accepted substrates include several flavonoids, hydroxycoumarins and beta-naphthols. Uses UDP-Glc as a sugar donor, but not UDP-Gal or UDP-GlcUA. May also be involved in detoxification of xenobiotics.1 Publication

    Catalytic activityi

    UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.1 Publication

    Kineticsi

    The kinetic constants are determined for the recombinant GST-fusion protein.1 Publication

    1. KM=3.5 µM for isorhamnetin1 Publication
    2. KM=0.6 mM for UDP-glucose1 Publication

    Vmax=2.8 nmol/sec/mg enzyme with isorhamnetin as substrate1 Publication

    Vmax=2.7 nmol/sec/mg enzyme with UDP-glucose as substrate1 Publication

    pH dependencei

    Optimum pH is 6.5.1 Publication

    Temperature dependencei

    Optimum temperature is 45 degrees Celsius.1 Publication

    GO - Molecular functioni

    1. flavonol 3-O-glucosyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucose flavonoid 3-O-glucosyltransferase 71 Publication (EC:2.4.1.911 Publication)
    Alternative name(s):
    Flavonol 3-O-glucosyltransferase 71 Publication
    Short name:
    FaGT71 Publication
    Gene namesi
    Name:GT7Imported
    OrganismiFragaria ananassa (Strawberry)
    Taxonomic identifieri3747 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeRosoideaePotentilleaeFragariinaeFragaria

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 487487UDP-glucose flavonoid 3-O-glucosyltransferase 7PRO_0000413770Add
    BLAST

    Expressioni

    Tissue specificityi

    Strongly expressed in achenes and receptacles.1 Publication

    Developmental stagei

    The expression in receptacles is ripening-related, with highest expression detected in red fruit.1 Publication

    Inductioni

    By de-achening. By injection with salicylic acid, with transcript levels increasing by a factor of 5-6 at 4 hours post-injection, remaining stable until 6 hours post-injection and falling below control levels at 8 hours post-injection. Down-regulated by synthetic auxin naphthaleneacetic acid (NAA).1 Publication

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the UDP-glycosyltransferase family.Sequence Analysis

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 1 hit.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]
    PROSITEiPS00375. UDPGT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2V6J9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAMETKSCQQ LHIFFLPFMA RGHSIPLTDI AKLFSSHGAR CTIVTTPLNA    50
    PLFSKATQRG EIELVLIKFP SAEAGLPQDC ESADLITTQD MLGKFVKATF 100
    LIEPHFEKIL DEHRPHCLVA DAFFTWATDV AAKFRIPRLY FHGTGFFALC 150
    ASLSVMMYQP HSNLSSDSES FVIPNLPDEI KMTRSQLPVF PDESEFMKML 200
    KASIEIEERS YGVIVNSFYE LEPAYANHYR KVFGRKAWHI GPVSFCNKAI 250
    EDKAERGSIK SSTAEKHECL KWLDSKKPRS VVYVSFGSMV RFADSQLLEI 300
    ATGLEASGQD FIWVVKKEKK EVEEWLPEGF EKRMEGKGLI IRDWAPQVLI 350
    LEHEAIGAFV THCGWNSILE AVSAGVPMIT WPVFGEQFYN EKLVTEIHRI 400
    GVPVGSEKWA LSFVDVNAET EGRVRREAIE EAVTRIMVGD EAVETRSRVK 450
    ELGENARRAV EEGGSSFLDL SALVGELNDL AFGGLVE 487
    Length:487
    Mass (Da):54,652
    Last modified:January 10, 2006 - v1
    Checksum:i313E7EB3889985FD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ289588 mRNA. Translation: ABB92749.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ289588 mRNA. Translation: ABB92749.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT1. Glycosyltransferase Family 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002213. UDP_glucos_trans.
    [Graphical view ]
    PANTHERi PTHR11926. PTHR11926. 1 hit.
    Pfami PF00201. UDPGT. 1 hit.
    [Graphical view ]
    PROSITEi PS00375. UDPGT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Multi-substrate flavonol O-glucosyltransferases from strawberry (Fragaria x ananassa) achene and receptacle."
      Griesser M., Vitzthum F., Fink B., Bellido M.L., Raasch C., Munoz-Blanco J., Schwab W.
      J. Exp. Bot. 59:2611-2625(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
      Strain: cv. Elsanta1 Publication.
      Tissue: Fruit1 Publication.

    Entry informationi

    Entry nameiUFOG7_FRAAN
    AccessioniPrimary (citable) accession number: Q2V6J9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2011
    Last sequence update: January 10, 2006
    Last modified: October 1, 2014
    This is version 28 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3