Q2V6J9 (UFOG7_FRAAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glucose flavonoid 3-O-glucosyltransferase 7
EC=2.4.1.91

Alternative name(s):
Flavonol 3-O-glucosyltransferase 7
Short name=FaGT7

Gene names

Name:

GT7

Organism

Fragaria ananassa (Strawberry)

Taxonomic identifier

3747 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Rosales › Rosaceae › Rosoideae › Potentilleae › Fragariinae › Fragaria

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Broad spectrum multifunctional glucosyltransferase. Catalyzes the formation of flavonol 3-O- and 4'-O-glucosides during fruit ripening. Accepted substrates include several flavonoids, hydroxycoumarins and beta-naphthols. Uses UDP-Glc as a sugar donor, but not UDP-Gal or UDP-GlcUA. May also be involved in detoxification of xenobiotics. Ref.1
Catalytic activity	UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside. Ref.1
Tissue specificity	Strongly expressed in achenes and receptacles. Ref.1
Developmental stage	The expression in receptacles is ripening-related, with highest expression detected in red fruit. Ref.1
Induction	By de-achening. By injection with salicylic acid, with transcript levels increasing by a factor of 5-6 at 4 hours post-injection, remaining stable until 6 hours post-injection and falling below control levels at 8 hours post-injection. Down-regulated by synthetic auxin naphthaleneacetic acid (NAA). Ref.1
Sequence similarities	Belongs to the UDP-glycosyltransferase family.
Biophysicochemical properties	Kinetic parameters: The kinetic constants are determined for the recombinant GST-fusion protein. Ref.1 KM=3.5 µM for isorhamnetin Ref.1 KM=0.6 mM for UDP-glucose Ref.1 Vmax=2.8 nmol/sec/mg enzyme with isorhamnetin as substrate Ref.1 Vmax=2.7 nmol/sec/mg enzyme with UDP-glucose as substrate Ref.1 pH dependence: Optimum pH is 6.5. Ref.1 Temperature dependence: Optimum temperature is 45 degrees Celsius. Ref.1

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Molecular_function	flavonol 3-O-glucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	UDP-glucose flavonoid 3-O-glucosyltransferase 7		PRO_0000413770

Sequences

	Sequence		Length	Mass (Da)
	Q2V6J9 [UniParc]. Last modified January 10, 2006. Version 1. Checksum: 313E7EB3889985FD	FASTA	487	54,652
	10 20 30 40 50 60 MAMETKSCQQ LHIFFLPFMA RGHSIPLTDI AKLFSSHGAR CTIVTTPLNA PLFSKATQRG 70 80 90 100 110 120 EIELVLIKFP SAEAGLPQDC ESADLITTQD MLGKFVKATF LIEPHFEKIL DEHRPHCLVA 130 140 150 160 170 180 DAFFTWATDV AAKFRIPRLY FHGTGFFALC ASLSVMMYQP HSNLSSDSES FVIPNLPDEI 190 200 210 220 230 240 KMTRSQLPVF PDESEFMKML KASIEIEERS YGVIVNSFYE LEPAYANHYR KVFGRKAWHI 250 260 270 280 290 300 GPVSFCNKAI EDKAERGSIK SSTAEKHECL KWLDSKKPRS VVYVSFGSMV RFADSQLLEI 310 320 330 340 350 360 ATGLEASGQD FIWVVKKEKK EVEEWLPEGF EKRMEGKGLI IRDWAPQVLI LEHEAIGAFV 370 380 390 400 410 420 THCGWNSILE AVSAGVPMIT WPVFGEQFYN EKLVTEIHRI GVPVGSEKWA LSFVDVNAET 430 440 450 460 470 480 EGRVRREAIE EAVTRIMVGD EAVETRSRVK ELGENARRAV EEGGSSFLDL SALVGELNDL AFGGLVE

References

[1]

"Multi-substrate flavonol O-glucosyltransferases from strawberry (Fragaria x ananassa) achene and receptacle." Griesser M., Vitzthum F., Fink B., Bellido M.L., Raasch C., Munoz-Blanco J., Schwab W. J. Exp. Bot. 59:2611-2625(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION. Strain: cv. Elsanta. Tissue: Fruit.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ289588 mRNA. Translation: ABB92749.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. PTHR11926. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UFOG7_FRAAN

Accession

Primary (citable) accession number: Q2V6J9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2011
Last sequence update:	January 10, 2006
Last modified:	October 3, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program