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Q2V4L8

- GUN3_ARATH

UniProt

Q2V4L8 - GUN3_ARATH

Protein

Endoglucanase 3

Gene

CEL5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 2 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    May be involved in the sloughing (cell-cell separation) of the root cap cells from root tip.1 Publication

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei402 – 4021PROSITE-ProRule annotation
    Active sitei453 – 4531PROSITE-ProRule annotation
    Active sitei462 – 4621PROSITE-ProRule annotation

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciARA:AT1G22880-MONOMER.
    ARA:GQT-2340-MONOMER.

    Protein family/group databases

    CAZyiGH9. Glycoside Hydrolase Family 9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase 3 (EC:3.2.1.4)
    Alternative name(s):
    Cellulase 5
    Short name:
    AtCEL5
    Endo-1,4-beta glucanase 3
    Gene namesi
    Name:CEL5
    Ordered Locus Names:At1g22880
    ORF Names:F19G10.16
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G22880.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. cell wall Source: TAIR
    2. extracellular region Source: UniProtKB-SubCell
    3. Golgi apparatus Source: TAIR
    4. plant-type cell wall Source: TAIR
    5. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 484463Endoglucanase 3PRO_0000249256Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi370 – 3701N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ2V4L8.
    PRIDEiQ2V4L8.

    Expressioni

    Tissue specificityi

    Specifically expressed in root cap cells.1 Publication

    Inductioni

    Down-regulated by auxin (IAA) and abscisic acid (ABA).1 Publication

    Gene expression databases

    GenevestigatoriQ2V4L8.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2V4L8.
    SMRiQ2V4L8. Positions 24-480.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG249374.
    HOGENOMiHOG000021033.
    InParanoidiQ2V4L8.
    OMAiTYINAAI.
    PhylomeDBiQ2V4L8.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view]
    PfamiPF00759. Glyco_hydro_9. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    PROSITEiPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q2V4L8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASPFFFVFL LSALSLENTY ASPNYREALS KSLLFFQGQR SGRLPSDQQL    50
    SWRSSSGLSD GSSAHVDLTG GYYDAGDNVK FNFPMAFTTT MLSWSSLEYG 100
    KKMGPELQNS RVAIRWATDY LLKCARATPG KLYVGVGDPN GDHKCWERPE 150
    DMDTPRTVYS VSPSNPGSDV AAETAAALAA SSMVFRKVDP KYSRLLLATA 200
    KKVMQFAIQY RGAYSNSLSS SVCPFYCSYS GYKDELLWGA AWLHRATNDP 250
    YYTNFIKSLG GGDQPDIFSW DNKYAGAYVL LSRRAVLNKD NNFELYKQAA 300
    ENFMCKILPN SPSSSTKYTK GGLMYKLPQS NLQYVTSITF LLTTYAKYMK 350
    STKQTFNCGN SLIVPNALIN LSKRQVDYVL GVNPMKMSYM VGFSSNFPKR 400
    IHHRGSSLPS RAVRSNSLGC NGGFQSFRTQ NPNPNILTGA IVGGPNQNDE 450
    YPDQRDDYTR SEPATYINAA FVGPLAYFAA SRSP 484
    Length:484
    Mass (Da):53,662
    Last modified:September 5, 2006 - v2
    Checksum:iFFB4FF2A83AE813B
    GO
    Isoform 2 (identifier: Q2V4L8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-84: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:400
    Mass (Da):44,428
    Checksum:i9F5D20F7997524BB
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 8484Missing in isoform 2. CuratedVSP_020386Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF000657 Genomic DNA. Translation: AAB72171.1.
    CP002684 Genomic DNA. Translation: AEE30299.1.
    CP002684 Genomic DNA. Translation: AEE30300.1.
    PIRiG86362.
    RefSeqiNP_001031082.1. NM_001036005.1. [Q2V4L8-2]
    NP_173701.1. NM_102134.2. [Q2V4L8-1]
    UniGeneiAt.41580.

    Genome annotation databases

    EnsemblPlantsiAT1G22880.1; AT1G22880.1; AT1G22880. [Q2V4L8-1]
    GeneIDi838893.
    KEGGiath:AT1G22880.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF000657 Genomic DNA. Translation: AAB72171.1 .
    CP002684 Genomic DNA. Translation: AEE30299.1 .
    CP002684 Genomic DNA. Translation: AEE30300.1 .
    PIRi G86362.
    RefSeqi NP_001031082.1. NM_001036005.1. [Q2V4L8-2 ]
    NP_173701.1. NM_102134.2. [Q2V4L8-1 ]
    UniGenei At.41580.

    3D structure databases

    ProteinModelPortali Q2V4L8.
    SMRi Q2V4L8. Positions 24-480.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH9. Glycoside Hydrolase Family 9.

    Proteomic databases

    PaxDbi Q2V4L8.
    PRIDEi Q2V4L8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G22880.1 ; AT1G22880.1 ; AT1G22880 . [Q2V4L8-1 ]
    GeneIDi 838893.
    KEGGi ath:AT1G22880.

    Organism-specific databases

    TAIRi AT1G22880.

    Phylogenomic databases

    eggNOGi NOG249374.
    HOGENOMi HOG000021033.
    InParanoidi Q2V4L8.
    OMAi TYINAAI.
    PhylomeDBi Q2V4L8.

    Enzyme and pathway databases

    BioCyci ARA:AT1G22880-MONOMER.
    ARA:GQT-2340-MONOMER.

    Gene expression databases

    Genevestigatori Q2V4L8.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view ]
    Pfami PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    PROSITEi PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
      Libertini E., Li Y., McQueen-Mason S.J.
      J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    4. "Root cap specific expression of an endo-beta-1,4-D-glucanase (cellulase): a new marker to study root development in Arabidopsis."
      del Campillo E., Abdel-Aziz A., Crawford D., Patterson S.E.
      Plant Mol. Biol. 56:309-323(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiGUN3_ARATH
    AccessioniPrimary (citable) accession number: Q2V4L8
    Secondary accession number(s): O23134
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 71 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The sloughing of root-cap cells from the root tip is a process of cell-cell separation that entails cell wall break down. It is important in plant development because it assists the growing root in penetrating the soil.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3