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Q2V4L8 (GUN3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase 3

EC=3.2.1.4
Alternative name(s):
Cellulase 5
Short name=AtCEL5
Endo-1,4-beta glucanase 3
Gene names
Name:CEL5
Ordered Locus Names:At1g22880
ORF Names:F19G10.16
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May be involved in the sloughing (cell-cell separation) of the root cap cells from root tip. Ref.4

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subcellular location

Secreted By similarity.

Tissue specificity

Specifically expressed in root cap cells. Ref.4

Induction

Down-regulated by auxin (IAA) and abscisic acid (ABA). Ref.4

Miscellaneous

The sloughing of root-cap cells from the root tip is a process of cell-cell separation that entails cell wall break down. It is important in plant development because it assists the growing root in penetrating the soil.

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q2V4L8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q2V4L8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-84: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 484463Endoglucanase 3
PRO_0000249256

Sites

Active site4021 By similarity
Active site4531 By similarity
Active site4621 By similarity

Amino acid modifications

Glycosylation3701N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 8484Missing in isoform 2.
VSP_020386

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 5, 2006. Version 2.
Checksum: FFB4FF2A83AE813B

FASTA48453,662
        10         20         30         40         50         60 
MASPFFFVFL LSALSLENTY ASPNYREALS KSLLFFQGQR SGRLPSDQQL SWRSSSGLSD 

        70         80         90        100        110        120 
GSSAHVDLTG GYYDAGDNVK FNFPMAFTTT MLSWSSLEYG KKMGPELQNS RVAIRWATDY 

       130        140        150        160        170        180 
LLKCARATPG KLYVGVGDPN GDHKCWERPE DMDTPRTVYS VSPSNPGSDV AAETAAALAA 

       190        200        210        220        230        240 
SSMVFRKVDP KYSRLLLATA KKVMQFAIQY RGAYSNSLSS SVCPFYCSYS GYKDELLWGA 

       250        260        270        280        290        300 
AWLHRATNDP YYTNFIKSLG GGDQPDIFSW DNKYAGAYVL LSRRAVLNKD NNFELYKQAA 

       310        320        330        340        350        360 
ENFMCKILPN SPSSSTKYTK GGLMYKLPQS NLQYVTSITF LLTTYAKYMK STKQTFNCGN 

       370        380        390        400        410        420 
SLIVPNALIN LSKRQVDYVL GVNPMKMSYM VGFSSNFPKR IHHRGSSLPS RAVRSNSLGC 

       430        440        450        460        470        480 
NGGFQSFRTQ NPNPNILTGA IVGGPNQNDE YPDQRDDYTR SEPATYINAA FVGPLAYFAA 


SRSP 

« Hide

Isoform 2 [UniParc].

Checksum: 9F5D20F7997524BB
Show »

FASTA40044,428

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
Libertini E., Li Y., McQueen-Mason S.J.
J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[4]"Root cap specific expression of an endo-beta-1,4-D-glucanase (cellulase): a new marker to study root development in Arabidopsis."
del Campillo E., Abdel-Aziz A., Crawford D., Patterson S.E.
Plant Mol. Biol. 56:309-323(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF000657 Genomic DNA. Translation: AAB72171.1.
CP002684 Genomic DNA. Translation: AEE30299.1.
CP002684 Genomic DNA. Translation: AEE30300.1.
PIRG86362.
RefSeqNP_001031082.1. NM_001036005.1. [Q2V4L8-2]
NP_173701.1. NM_102134.2. [Q2V4L8-1]
UniGeneAt.41580.

3D structure databases

ProteinModelPortalQ2V4L8.
SMRQ2V4L8. Positions 24-480.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH9. Glycoside Hydrolase Family 9.

Proteomic databases

PaxDbQ2V4L8.
PRIDEQ2V4L8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G22880.1; AT1G22880.1; AT1G22880. [Q2V4L8-1]
GeneID838893.
KEGGath:AT1G22880.

Organism-specific databases

TAIRAT1G22880.

Phylogenomic databases

eggNOGNOG249374.
HOGENOMHOG000021033.
InParanoidQ2V4L8.
OMATYINAAI.
PhylomeDBQ2V4L8.

Enzyme and pathway databases

BioCycARA:AT1G22880-MONOMER.
ARA:GQT-2340-MONOMER.

Gene expression databases

GenevestigatorQ2V4L8.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
PROSITEPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN3_ARATH
AccessionPrimary (citable) accession number: Q2V4L8
Secondary accession number(s): O23134
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: May 14, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names