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Protein

Endoglucanase 3

Gene

CEL5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May be involved in the sloughing (cell-cell separation) of the root cap cells from root tip.1 Publication

Miscellaneous

The sloughing of root-cap cells from the root tip is a process of cell-cell separation that entails cell wall break down. It is important in plant development because it assists the growing root in penetrating the soil.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei402PROSITE-ProRule annotation1
Active sitei453PROSITE-ProRule annotation1
Active sitei462PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciARA:AT1G22880-MONOMER.

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 3 (EC:3.2.1.4)
Alternative name(s):
Cellulase 5
Short name:
AtCEL5
Endo-1,4-beta glucanase 3
Gene namesi
Name:CEL5
Ordered Locus Names:At1g22880
ORF Names:F19G10.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G22880.
TAIRilocus:2017704. AT1G22880.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000024925622 – 484Endoglucanase 3Add BLAST463

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi370N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ2V4L8.
PRIDEiQ2V4L8.

Expressioni

Tissue specificityi

Specifically expressed in root cap cells.1 Publication

Inductioni

Down-regulated by auxin (IAA) and abscisic acid (ABA).1 Publication

Gene expression databases

ExpressionAtlasiQ2V4L8. baseline and differential.
GenevisibleiQ2V4L8. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G22880.1.

Structurei

3D structure databases

ProteinModelPortaliQ2V4L8.
SMRiQ2V4L8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IIWR. Eukaryota.
ENOG410YA4V. LUCA.
HOGENOMiHOG000021033.
InParanoidiQ2V4L8.
OMAiFNCGNSL.
OrthoDBiEOG093608GU.
PhylomeDBiQ2V4L8.

Family and domain databases

InterProiView protein in InterPro
IPR008928. 6-hairpin_glycosidase-like.
IPR001701. Glyco_hydro_9.
IPR033126. Glyco_hydro_9_Asp/Glu_AS.
PfamiView protein in Pfam
PF00759. Glyco_hydro_9. 1 hit.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiView protein in PROSITE
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2V4L8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPFFFVFL LSALSLENTY ASPNYREALS KSLLFFQGQR SGRLPSDQQL
60 70 80 90 100
SWRSSSGLSD GSSAHVDLTG GYYDAGDNVK FNFPMAFTTT MLSWSSLEYG
110 120 130 140 150
KKMGPELQNS RVAIRWATDY LLKCARATPG KLYVGVGDPN GDHKCWERPE
160 170 180 190 200
DMDTPRTVYS VSPSNPGSDV AAETAAALAA SSMVFRKVDP KYSRLLLATA
210 220 230 240 250
KKVMQFAIQY RGAYSNSLSS SVCPFYCSYS GYKDELLWGA AWLHRATNDP
260 270 280 290 300
YYTNFIKSLG GGDQPDIFSW DNKYAGAYVL LSRRAVLNKD NNFELYKQAA
310 320 330 340 350
ENFMCKILPN SPSSSTKYTK GGLMYKLPQS NLQYVTSITF LLTTYAKYMK
360 370 380 390 400
STKQTFNCGN SLIVPNALIN LSKRQVDYVL GVNPMKMSYM VGFSSNFPKR
410 420 430 440 450
IHHRGSSLPS RAVRSNSLGC NGGFQSFRTQ NPNPNILTGA IVGGPNQNDE
460 470 480
YPDQRDDYTR SEPATYINAA FVGPLAYFAA SRSP
Length:484
Mass (Da):53,662
Last modified:September 5, 2006 - v2
Checksum:iFFB4FF2A83AE813B
GO
Isoform 2 (identifier: Q2V4L8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-84: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:400
Mass (Da):44,428
Checksum:i9F5D20F7997524BB
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0203861 – 84Missing in isoform 2. CuratedAdd BLAST84

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000657 Genomic DNA. Translation: AAB72171.1.
CP002684 Genomic DNA. Translation: AEE30299.1.
CP002684 Genomic DNA. Translation: AEE30300.1.
PIRiG86362.
RefSeqiNP_001031082.1. NM_001036005.2. [Q2V4L8-2]
NP_173701.1. NM_102134.3. [Q2V4L8-1]
UniGeneiAt.41580.

Genome annotation databases

EnsemblPlantsiAT1G22880.1; AT1G22880.1; AT1G22880. [Q2V4L8-1]
AT1G22880.2; AT1G22880.2; AT1G22880. [Q2V4L8-2]
GeneIDi838893.
GrameneiAT1G22880.1; AT1G22880.1; AT1G22880.
AT1G22880.2; AT1G22880.2; AT1G22880.
KEGGiath:AT1G22880.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGUN3_ARATH
AccessioniPrimary (citable) accession number: Q2V4L8
Secondary accession number(s): O23134
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: October 25, 2017
This is version 90 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families