Q2V4L8 (GUN3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 3
EC=3.2.1.4

Alternative name(s):
Cellulase 5
Short name=AtCEL5
Endo-1,4-beta glucanase 3

Gene names

Name:	CEL5
Ordered Locus Names:	At1g22880
ORF Names:	F19G10.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in the sloughing (cell-cell separation) of the root cap cells from root tip. Ref.4
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Tissue specificity	Specifically expressed in root cap cells. Ref.4
Induction	Down-regulated by auxin (IAA) and abscisic acid (ABA). Ref.4
Miscellaneous	The sloughing of root-cap cells from the root tip is a process of cell-cell separation that entails cell wall break down. It is important in plant development because it assists the growing root in penetrating the soil.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 22430844. Source: TAIR extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell plant-type cell wall Inferred from direct assay PubMed 16287169. Source: TAIR plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q2V4L8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q2V4L8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-84: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 484	463	Endoglucanase 3		PRO_0000249256
Sites
	Active site	402	1	By similarity
	Active site	453	1	By similarity
	Active site	462	1	By similarity
Amino acid modifications
	Glycosylation	370	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 84	84	Missing in isoform 2.		VSP_020386

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 5, 2006. Version 2. Checksum: FFB4FF2A83AE813B	FASTA	484	53,662
	10 20 30 40 50 60 MASPFFFVFL LSALSLENTY ASPNYREALS KSLLFFQGQR SGRLPSDQQL SWRSSSGLSD 70 80 90 100 110 120 GSSAHVDLTG GYYDAGDNVK FNFPMAFTTT MLSWSSLEYG KKMGPELQNS RVAIRWATDY 130 140 150 160 170 180 LLKCARATPG KLYVGVGDPN GDHKCWERPE DMDTPRTVYS VSPSNPGSDV AAETAAALAA 190 200 210 220 230 240 SSMVFRKVDP KYSRLLLATA KKVMQFAIQY RGAYSNSLSS SVCPFYCSYS GYKDELLWGA 250 260 270 280 290 300 AWLHRATNDP YYTNFIKSLG GGDQPDIFSW DNKYAGAYVL LSRRAVLNKD NNFELYKQAA 310 320 330 340 350 360 ENFMCKILPN SPSSSTKYTK GGLMYKLPQS NLQYVTSITF LLTTYAKYMK STKQTFNCGN 370 380 390 400 410 420 SLIVPNALIN LSKRQVDYVL GVNPMKMSYM VGFSSNFPKR IHHRGSSLPS RAVRSNSLGC 430 440 450 460 470 480 NGGFQSFRTQ NPNPNILTGA IVGGPNQNDE YPDQRDDYTR SEPATYINAA FVGPLAYFAA SRSP
	Isoform 2 [UniParc]. Checksum: 9F5D20F7997524BB	FASTA	400	44,428
	10 20 30 40 50 60 MAFTTTMLSW SSLEYGKKMG PELQNSRVAI RWATDYLLKC ARATPGKLYV GVGDPNGDHK 70 80 90 100 110 120 CWERPEDMDT PRTVYSVSPS NPGSDVAAET AAALAASSMV FRKVDPKYSR LLLATAKKVM 130 140 150 160 170 180 QFAIQYRGAY SNSLSSSVCP FYCSYSGYKD ELLWGAAWLH RATNDPYYTN FIKSLGGGDQ 190 200 210 220 230 240 PDIFSWDNKY AGAYVLLSRR AVLNKDNNFE LYKQAAENFM CKILPNSPSS STKYTKGGLM 250 260 270 280 290 300 YKLPQSNLQY VTSITFLLTT YAKYMKSTKQ TFNCGNSLIV PNALINLSKR QVDYVLGVNP 310 320 330 340 350 360 MKMSYMVGFS SNFPKRIHHR GSSLPSRAVR SNSLGCNGGF QSFRTQNPNP NILTGAIVGG 370 380 390 400 PNQNDEYPDQ RDDYTRSEPA TYINAAFVGP LAYFAASRSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family." Libertini E., Li Y., McQueen-Mason S.J. J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[4]	"Root cap specific expression of an endo-beta-1,4-D-glucanase (cellulase): a new marker to study root development in Arabidopsis." del Campillo E., Abdel-Aziz A., Crawford D., Patterson S.E. Plant Mol. Biol. 56:309-323(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF000657 Genomic DNA. Translation: AAB72171.1. CP002684 Genomic DNA. Translation: AEE30299.1. CP002684 Genomic DNA. Translation: AEE30300.1.
IPI	IPI00537156. IPI00656697.
PIR	G86362.
RefSeq	NP_001031082.1. NM_001036005.1. NP_173701.1. NM_102134.2.
UniGene	At.41580.
3D structure databases
ProteinModelPortal	Q2V4L8.
SMR	Q2V4L8. Positions 24-480.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Proteomic databases
PaxDb	Q2V4L8.
PRIDE	Q2V4L8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G22880.1; AT1G22880.1; AT1G22880.
GeneID	838893.
KEGG	ath:AT1G22880.
Organism-specific databases
TAIR	At1g22880.
Phylogenomic databases
eggNOG	NOG249374.
HOGENOM	HOG000021033.
InParanoid	Q2V4L8.
OMA	KSILFFQ.
PhylomeDB	Q2V4L8.
ProtClustDB	PLN02175.
Gene expression databases
Genevestigator	Q2V4L8.
GermOnline	AT1G22880. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. False negative. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN3_ARATH

Accession

Primary (citable) accession number: Q2V4L8
Secondary accession number(s): O23134

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	September 5, 2006
Last modified:	May 1, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program