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Q2V4L8

- GUN3_ARATH

UniProt

Q2V4L8 - GUN3_ARATH

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Protein

Endoglucanase 3

Gene

CEL5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

May be involved in the sloughing (cell-cell separation) of the root cap cells from root tip.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei402 – 4021PROSITE-ProRule annotation
Active sitei453 – 4531PROSITE-ProRule annotation
Active sitei462 – 4621PROSITE-ProRule annotation

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
  2. cell wall organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciARA:AT1G22880-MONOMER.
ARA:GQT-2340-MONOMER.

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 3 (EC:3.2.1.4)
Alternative name(s):
Cellulase 5
Short name:
AtCEL5
Endo-1,4-beta glucanase 3
Gene namesi
Name:CEL5
Ordered Locus Names:At1g22880
ORF Names:F19G10.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G22880.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. cell wall Source: TAIR
  2. extracellular region Source: UniProtKB-KW
  3. Golgi apparatus Source: TAIR
  4. plant-type cell wall Source: TAIR
  5. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 484463Endoglucanase 3PRO_0000249256Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi370 – 3701N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ2V4L8.
PRIDEiQ2V4L8.

Expressioni

Tissue specificityi

Specifically expressed in root cap cells.1 Publication

Inductioni

Down-regulated by auxin (IAA) and abscisic acid (ABA).1 Publication

Gene expression databases

GenevestigatoriQ2V4L8.

Structurei

3D structure databases

ProteinModelPortaliQ2V4L8.
SMRiQ2V4L8. Positions 24-480.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG249374.
HOGENOMiHOG000021033.
InParanoidiQ2V4L8.
OMAiTYINAAI.
PhylomeDBiQ2V4L8.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q2V4L8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPFFFVFL LSALSLENTY ASPNYREALS KSLLFFQGQR SGRLPSDQQL
60 70 80 90 100
SWRSSSGLSD GSSAHVDLTG GYYDAGDNVK FNFPMAFTTT MLSWSSLEYG
110 120 130 140 150
KKMGPELQNS RVAIRWATDY LLKCARATPG KLYVGVGDPN GDHKCWERPE
160 170 180 190 200
DMDTPRTVYS VSPSNPGSDV AAETAAALAA SSMVFRKVDP KYSRLLLATA
210 220 230 240 250
KKVMQFAIQY RGAYSNSLSS SVCPFYCSYS GYKDELLWGA AWLHRATNDP
260 270 280 290 300
YYTNFIKSLG GGDQPDIFSW DNKYAGAYVL LSRRAVLNKD NNFELYKQAA
310 320 330 340 350
ENFMCKILPN SPSSSTKYTK GGLMYKLPQS NLQYVTSITF LLTTYAKYMK
360 370 380 390 400
STKQTFNCGN SLIVPNALIN LSKRQVDYVL GVNPMKMSYM VGFSSNFPKR
410 420 430 440 450
IHHRGSSLPS RAVRSNSLGC NGGFQSFRTQ NPNPNILTGA IVGGPNQNDE
460 470 480
YPDQRDDYTR SEPATYINAA FVGPLAYFAA SRSP
Length:484
Mass (Da):53,662
Last modified:September 5, 2006 - v2
Checksum:iFFB4FF2A83AE813B
GO
Isoform 2 (identifier: Q2V4L8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-84: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:400
Mass (Da):44,428
Checksum:i9F5D20F7997524BB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8484Missing in isoform 2. CuratedVSP_020386Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000657 Genomic DNA. Translation: AAB72171.1.
CP002684 Genomic DNA. Translation: AEE30299.1.
CP002684 Genomic DNA. Translation: AEE30300.1.
PIRiG86362.
RefSeqiNP_001031082.1. NM_001036005.1. [Q2V4L8-2]
NP_173701.1. NM_102134.2. [Q2V4L8-1]
UniGeneiAt.41580.

Genome annotation databases

EnsemblPlantsiAT1G22880.1; AT1G22880.1; AT1G22880. [Q2V4L8-1]
GeneIDi838893.
KEGGiath:AT1G22880.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000657 Genomic DNA. Translation: AAB72171.1 .
CP002684 Genomic DNA. Translation: AEE30299.1 .
CP002684 Genomic DNA. Translation: AEE30300.1 .
PIRi G86362.
RefSeqi NP_001031082.1. NM_001036005.1. [Q2V4L8-2 ]
NP_173701.1. NM_102134.2. [Q2V4L8-1 ]
UniGenei At.41580.

3D structure databases

ProteinModelPortali Q2V4L8.
SMRi Q2V4L8. Positions 24-480.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH9. Glycoside Hydrolase Family 9.

Proteomic databases

PaxDbi Q2V4L8.
PRIDEi Q2V4L8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G22880.1 ; AT1G22880.1 ; AT1G22880 . [Q2V4L8-1 ]
GeneIDi 838893.
KEGGi ath:AT1G22880.

Organism-specific databases

TAIRi AT1G22880.

Phylogenomic databases

eggNOGi NOG249374.
HOGENOMi HOG000021033.
InParanoidi Q2V4L8.
OMAi TYINAAI.
PhylomeDBi Q2V4L8.

Enzyme and pathway databases

BioCyci ARA:AT1G22880-MONOMER.
ARA:GQT-2340-MONOMER.

Gene expression databases

Genevestigatori Q2V4L8.

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view ]
Pfami PF00759. Glyco_hydro_9. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
PROSITEi PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
    Libertini E., Li Y., McQueen-Mason S.J.
    J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  4. "Root cap specific expression of an endo-beta-1,4-D-glucanase (cellulase): a new marker to study root development in Arabidopsis."
    del Campillo E., Abdel-Aziz A., Crawford D., Patterson S.E.
    Plant Mol. Biol. 56:309-323(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiGUN3_ARATH
AccessioniPrimary (citable) accession number: Q2V4L8
Secondary accession number(s): O23134
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: October 29, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The sloughing of root-cap cells from the root tip is a process of cell-cell separation that entails cell wall break down. It is important in plant development because it assists the growing root in penetrating the soil.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3