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Q2V419 (CKB12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase B1-2
Short name=CDKB1;2
EC=2.7.11.22
EC=2.7.11.23
Gene names
Name:CDKB1-2
Ordered Locus Names:At2g38620
ORF Names:T6A23.18
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Subunit structure

Interacts with CKS1. Ref.1

Tissue specificity

Expressed in flowers. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence BAD95353.1 differs from that shown. Reason: Erroneous termination at position 60. Translated as Gln.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q2V419-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q2V419-2)

The sequence of this isoform differs from the canonical sequence as follows:
     236-257: LLGTPTEQQWPGVMALRDWHVY → YLLLVFDANVYVYRNQFRTLAH
     258-311: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 311311Cyclin-dependent kinase B1-2
PRO_0000293114

Regions

Domain4 – 303300Protein kinase
Nucleotide binding10 – 189ATP By similarity

Sites

Active site1441Proton acceptor By similarity
Binding site331ATP By similarity

Amino acid modifications

Modified residue141Phosphothreonine By similarity
Modified residue151Phosphotyrosine By similarity
Modified residue1781Phosphothreonine By similarity

Natural variations

Alternative sequence236 – 25722LLGTP…DWHVY → YLLLVFDANVYVYRNQFRTL AH in isoform 2.
VSP_026473
Alternative sequence258 – 31154Missing in isoform 2.
VSP_026474

Experimental info

Sequence conflict2311L → I in BAD95353. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: A04E60AEF83E5BB0

FASTA31135,582
        10         20         30         40         50         60 
MEKYEKLEKV GEGTYGKVYK AMEKTTGKLV ALKKTRLEMD EEGIPPTALR EISLLQMLSQ 

        70         80         90        100        110        120 
SIYIVRLLCV EHVIQSKDST VSHSPKSNLY LVFEYLDTDL KKFIDSHRKG SNPRPLEASL 

       130        140        150        160        170        180 
VQRFMFQLFK GVAHCHSHGV LHRDLKPQNL LLDKDKGILK IADLGLSRAF TVPLKAYTHE 

       190        200        210        220        230        240 
IVTLWYRAPE VLLGSTHYST AVDIWSVGCI FAEMIRRQAL FPGDSEFQQL LHIFRLLGTP 

       250        260        270        280        290        300 
TEQQWPGVMA LRDWHVYPKW EPQDLSRAVP SLSPEGIDLL TQMLKYNPAE RISAKAALDH 

       310 
PYFDSLDKSQ F

« Hide

Isoform 2 [UniParc].

Checksum: 45178C9E1D5852B3
Show »

FASTA25729,602

References

« Hide 'large scale' references
[1]"Identification of novel cyclin-dependent kinases interacting with the CKS1 protein of Arabidopsis."
Boudolf V., Rombauts S., Naudts M., Inze D., de Veylder L.
J. Exp. Bot. 52:1381-1382(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH CKS1.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Genome-wide analysis of core cell cycle genes in Arabidopsis."
Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.
Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[6]"Cell cycle regulation in plant development."
Inze D., de Veylder L.
Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ297937 mRNA. Translation: CAC34053.1.
AC005499 Genomic DNA. Translation: AAC67356.1.
CP002685 Genomic DNA. Translation: AEC09557.1.
CP002685 Genomic DNA. Translation: AEC09558.1.
AK221669 mRNA. Translation: BAD95353.1. Sequence problems.
IPIIPI00535858.
IPI00656545.
PIRC84807.
RefSeqNP_001031507.1. NM_001036430.2.
NP_181396.2. NM_129419.3.
UniGeneAt.10323.
At.37270.

3D structure databases

ProteinModelPortalQ2V419.
SMRQ2V419. Positions 1-308.
ModBaseSearch...

Protein-protein interaction databases

IntActQ2V419. 9 interactions.

Proteomic databases

PaxDbQ2V419.
PRIDEQ2V419.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G38620.2; AT2G38620.2; AT2G38620.
GeneID818444.
KEGGath:AT2G38620.

Organism-specific databases

GeneFarm2965. 107.
TAIRAt2g38620.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
OMARITCEDA.
PhylomeDBQ2V419.
ProtClustDBCLSN2685082.

Gene expression databases

GenevestigatorQ2V419.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCKB12_ARATH
AccessionPrimary (citable) accession number: Q2V419
Secondary accession number(s): Q56XK5, Q9ZVI4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: May 29, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents