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Protein

Cyclin-dependent kinase B1-2

Gene

CDKB1-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPPROSITE-ProRule annotation1
Active sitei144Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • regulation of cell cycle Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase B1-2 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKB1;2
Gene namesi
Name:CDKB1-2
Ordered Locus Names:At2g38620
ORF Names:T6A23.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G38620.

Subcellular locationi

GO - Cellular componenti

  • cyclin-dependent protein kinase holoenzyme complex Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002931141 – 311Cyclin-dependent kinase B1-2Add BLAST311

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphotyrosineBy similarity1
Modified residuei178PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ2V419.

Expressioni

Tissue specificityi

Expressed in flowers.1 Publication

Gene expression databases

GenevisibleiQ2V419. AT.

Interactioni

Subunit structurei

Interacts with CKS1.1 Publication

Protein-protein interaction databases

BioGridi3786. 40 interactors.
IntActiQ2V419. 9 interactors.
STRINGi3702.AT2G38620.2.

Structurei

3D structure databases

ProteinModelPortaliQ2V419.
SMRiQ2V419.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 303Protein kinasePROSITE-ProRule annotationAdd BLAST300

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ2V419.
KOiK07760.
OMAiPQWEPQN.
OrthoDBiEOG09360F64.
PhylomeDBiQ2V419.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2V419-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKYEKLEKV GEGTYGKVYK AMEKTTGKLV ALKKTRLEMD EEGIPPTALR
60 70 80 90 100
EISLLQMLSQ SIYIVRLLCV EHVIQSKDST VSHSPKSNLY LVFEYLDTDL
110 120 130 140 150
KKFIDSHRKG SNPRPLEASL VQRFMFQLFK GVAHCHSHGV LHRDLKPQNL
160 170 180 190 200
LLDKDKGILK IADLGLSRAF TVPLKAYTHE IVTLWYRAPE VLLGSTHYST
210 220 230 240 250
AVDIWSVGCI FAEMIRRQAL FPGDSEFQQL LHIFRLLGTP TEQQWPGVMA
260 270 280 290 300
LRDWHVYPKW EPQDLSRAVP SLSPEGIDLL TQMLKYNPAE RISAKAALDH
310
PYFDSLDKSQ F
Length:311
Mass (Da):35,582
Last modified:July 10, 2007 - v2
Checksum:iA04E60AEF83E5BB0
GO
Isoform 2 (identifier: Q2V419-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     236-257: LLGTPTEQQWPGVMALRDWHVY → YLLLVFDANVYVYRNQFRTLAH
     258-311: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:257
Mass (Da):29,602
Checksum:i45178C9E1D5852B3
GO

Sequence cautioni

The sequence BAD95353 differs from that shown. Reason: Erroneous termination at position 60. Translated as Gln.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti231L → I in BAD95353 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_026473236 – 257LLGTP…DWHVY → YLLLVFDANVYVYRNQFRTL AH in isoform 2. CuratedAdd BLAST22
Alternative sequenceiVSP_026474258 – 311Missing in isoform 2. CuratedAdd BLAST54

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ297937 mRNA. Translation: CAC34053.1.
AC005499 Genomic DNA. Translation: AAC67356.1.
CP002685 Genomic DNA. Translation: AEC09557.1.
CP002685 Genomic DNA. Translation: AEC09558.1.
AK221669 mRNA. Translation: BAD95353.1. Sequence problems.
PIRiC84807.
RefSeqiNP_001031507.1. NM_001036430.3. [Q2V419-1]
NP_181396.2. NM_129419.3. [Q2V419-2]
UniGeneiAt.10323.
At.37270.

Genome annotation databases

EnsemblPlantsiAT2G38620.2; AT2G38620.2; AT2G38620. [Q2V419-1]
GeneIDi818444.
GrameneiAT2G38620.2; AT2G38620.2; AT2G38620.
KEGGiath:AT2G38620.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ297937 mRNA. Translation: CAC34053.1.
AC005499 Genomic DNA. Translation: AAC67356.1.
CP002685 Genomic DNA. Translation: AEC09557.1.
CP002685 Genomic DNA. Translation: AEC09558.1.
AK221669 mRNA. Translation: BAD95353.1. Sequence problems.
PIRiC84807.
RefSeqiNP_001031507.1. NM_001036430.3. [Q2V419-1]
NP_181396.2. NM_129419.3. [Q2V419-2]
UniGeneiAt.10323.
At.37270.

3D structure databases

ProteinModelPortaliQ2V419.
SMRiQ2V419.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3786. 40 interactors.
IntActiQ2V419. 9 interactors.
STRINGi3702.AT2G38620.2.

Proteomic databases

PaxDbiQ2V419.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G38620.2; AT2G38620.2; AT2G38620. [Q2V419-1]
GeneIDi818444.
GrameneiAT2G38620.2; AT2G38620.2; AT2G38620.
KEGGiath:AT2G38620.

Organism-specific databases

TAIRiAT2G38620.

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ2V419.
KOiK07760.
OMAiPQWEPQN.
OrthoDBiEOG09360F64.
PhylomeDBiQ2V419.

Miscellaneous databases

PROiQ2V419.

Gene expression databases

GenevisibleiQ2V419. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCKB12_ARATH
AccessioniPrimary (citable) accession number: Q2V419
Secondary accession number(s): Q56XK5, Q9ZVI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: November 30, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.