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Q2V419

- CKB12_ARATH

UniProt

Q2V419 - CKB12_ARATH

Protein

Cyclin-dependent kinase B1-2

Gene

CDKB1-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 2 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Active sitei144 – 1441Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin binding Source: TAIR
    3. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
    4. protein binding Source: TAIR
    5. protein serine/threonine kinase activity Source: TAIR
    6. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. regulation of cell cycle Source: TAIR

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G38620-MONOMER.
    ARA:GQT-1788-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase B1-2 (EC:2.7.11.22, EC:2.7.11.23)
    Short name:
    CDKB1;2
    Gene namesi
    Name:CDKB1-2
    Ordered Locus Names:At2g38620
    ORF Names:T6A23.18
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G38620.

    Subcellular locationi

    GO - Cellular componenti

    1. cyclin-dependent protein kinase holoenzyme complex Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 311311Cyclin-dependent kinase B1-2PRO_0000293114Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei15 – 151PhosphotyrosineBy similarity
    Modified residuei178 – 1781PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ2V419.
    PRIDEiQ2V419.

    Expressioni

    Tissue specificityi

    Expressed in flowers.1 Publication

    Gene expression databases

    GenevestigatoriQ2V419.

    Interactioni

    Subunit structurei

    Interacts with CKS1.1 Publication

    Protein-protein interaction databases

    BioGridi3786. 40 interactions.
    IntActiQ2V419. 9 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2V419.
    SMRiQ2V419. Positions 1-308.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 303300Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    KOiK07760.
    OMAiCALRDWH.
    PhylomeDBiQ2V419.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q2V419-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEKYEKLEKV GEGTYGKVYK AMEKTTGKLV ALKKTRLEMD EEGIPPTALR    50
    EISLLQMLSQ SIYIVRLLCV EHVIQSKDST VSHSPKSNLY LVFEYLDTDL 100
    KKFIDSHRKG SNPRPLEASL VQRFMFQLFK GVAHCHSHGV LHRDLKPQNL 150
    LLDKDKGILK IADLGLSRAF TVPLKAYTHE IVTLWYRAPE VLLGSTHYST 200
    AVDIWSVGCI FAEMIRRQAL FPGDSEFQQL LHIFRLLGTP TEQQWPGVMA 250
    LRDWHVYPKW EPQDLSRAVP SLSPEGIDLL TQMLKYNPAE RISAKAALDH 300
    PYFDSLDKSQ F 311
    Length:311
    Mass (Da):35,582
    Last modified:July 10, 2007 - v2
    Checksum:iA04E60AEF83E5BB0
    GO
    Isoform 2 (identifier: Q2V419-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         236-257: LLGTPTEQQWPGVMALRDWHVY → YLLLVFDANVYVYRNQFRTLAH
         258-311: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:257
    Mass (Da):29,602
    Checksum:i45178C9E1D5852B3
    GO

    Sequence cautioni

    The sequence BAD95353.1 differs from that shown. Reason: Erroneous termination at position 60. Translated as Gln.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti231 – 2311L → I in BAD95353. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei236 – 25722LLGTP…DWHVY → YLLLVFDANVYVYRNQFRTL AH in isoform 2. CuratedVSP_026473Add
    BLAST
    Alternative sequencei258 – 31154Missing in isoform 2. CuratedVSP_026474Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ297937 mRNA. Translation: CAC34053.1.
    AC005499 Genomic DNA. Translation: AAC67356.1.
    CP002685 Genomic DNA. Translation: AEC09557.1.
    CP002685 Genomic DNA. Translation: AEC09558.1.
    AK221669 mRNA. Translation: BAD95353.1. Sequence problems.
    PIRiC84807.
    RefSeqiNP_001031507.1. NM_001036430.2. [Q2V419-1]
    NP_181396.2. NM_129419.3. [Q2V419-2]
    UniGeneiAt.10323.
    At.37270.

    Genome annotation databases

    EnsemblPlantsiAT2G38620.2; AT2G38620.2; AT2G38620. [Q2V419-1]
    GeneIDi818444.
    KEGGiath:AT2G38620.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ297937 mRNA. Translation: CAC34053.1 .
    AC005499 Genomic DNA. Translation: AAC67356.1 .
    CP002685 Genomic DNA. Translation: AEC09557.1 .
    CP002685 Genomic DNA. Translation: AEC09558.1 .
    AK221669 mRNA. Translation: BAD95353.1 . Sequence problems.
    PIRi C84807.
    RefSeqi NP_001031507.1. NM_001036430.2. [Q2V419-1 ]
    NP_181396.2. NM_129419.3. [Q2V419-2 ]
    UniGenei At.10323.
    At.37270.

    3D structure databases

    ProteinModelPortali Q2V419.
    SMRi Q2V419. Positions 1-308.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 3786. 40 interactions.
    IntActi Q2V419. 9 interactions.

    Proteomic databases

    PaxDbi Q2V419.
    PRIDEi Q2V419.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G38620.2 ; AT2G38620.2 ; AT2G38620 . [Q2V419-1 ]
    GeneIDi 818444.
    KEGGi ath:AT2G38620.

    Organism-specific databases

    GeneFarmi 2965. 107.
    TAIRi AT2G38620.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    KOi K07760.
    OMAi CALRDWH.
    PhylomeDBi Q2V419.

    Enzyme and pathway databases

    BioCyci ARA:AT2G38620-MONOMER.
    ARA:GQT-1788-MONOMER.

    Gene expression databases

    Genevestigatori Q2V419.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of novel cyclin-dependent kinases interacting with the CKS1 protein of Arabidopsis."
      Boudolf V., Rombauts S., Naudts M., Inze D., de Veylder L.
      J. Exp. Bot. 52:1381-1382(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH CKS1.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Genome-wide analysis of core cell cycle genes in Arabidopsis."
      Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.
      Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Cell cycle regulation in plant development."
      Inze D., de Veylder L.
      Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiCKB12_ARATH
    AccessioniPrimary (citable) accession number: Q2V419
    Secondary accession number(s): Q56XK5, Q9ZVI4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 75 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3