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Protein

Cyclin-dependent kinase B1-2

Gene

CDKB1-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Together with CDKB1-1, promotes both the last division in the stomatal cell lineage as well as the number of stomata (PubMed:20675570). In collaboration with MYB124 and MYB88, restrict the G1/S transition and chloroplast and nuclear number during stomatal formation, and normally maintain fate and developmental progression throughout the stomatal cell lineage (PubMed:24123248).2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPPROSITE-ProRule annotation1
Active sitei144Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • G1/S transition of mitotic cell cycle Source: GO_Central
  • guard mother cell differentiation Source: UniProtKB
  • positive regulation of cell proliferation Source: GO_Central
  • protein phosphorylation Source: GO_Central
  • regulation of cell cycle Source: TAIR
  • regulation of cell cycle G1/S phase transition Source: UniProtKB
  • regulation of DNA endoreduplication Source: UniProtKB
  • regulation of G2/M transition of mitotic cell cycle Source: GO_Central
  • regulation of gene expression Source: GO_Central
  • regulation of stomatal complex patterning Source: UniProtKB
  • response to organic substance Source: GO_Central
  • signal transduction Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase B1-2 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKB1;2
Gene namesi
Name:CDKB1-2
Ordered Locus Names:At2g38620
ORF Names:T6A23.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G38620.
TAIRilocus:2064087. AT2G38620.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

No apparent stomatal abnormalities. The double mutant cdkb1;1 cdkb1;2 has a reduced number of abnormal stomata consisting in single guard cells (GC) (PubMed:20675570). The quadruple mutant flp-1 myb88 cdkb1;1 cdkb1;2 has a reduced number of large single guard cells blocked at mitosis, with strongly altered shape and size and characterized by enlarged nucleus due to endomitosis and endocycling, as well as extensive chloroplast replication (PubMed:24123248).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002931141 – 311Cyclin-dependent kinase B1-2Add BLAST311

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphotyrosineBy similarity1
Modified residuei178PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ2V419.

Expressioni

Tissue specificityi

Expressed in flowers.1 Publication

Gene expression databases

ExpressionAtlasiQ2V419. baseline and differential.
GenevisibleiQ2V419. AT.

Interactioni

Subunit structurei

Interacts with CKS1.1 Publication

Protein-protein interaction databases

BioGridi3786. 40 interactors.
IntActiQ2V419. 9 interactors.
STRINGi3702.AT2G38620.2.

Structurei

3D structure databases

ProteinModelPortaliQ2V419.
SMRiQ2V419.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 303Protein kinasePROSITE-ProRule annotationAdd BLAST300

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ2V419.
KOiK07760.
OMAiDWHVYPQ.
OrthoDBiEOG09360F64.
PhylomeDBiQ2V419.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2V419-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKYEKLEKV GEGTYGKVYK AMEKTTGKLV ALKKTRLEMD EEGIPPTALR
60 70 80 90 100
EISLLQMLSQ SIYIVRLLCV EHVIQSKDST VSHSPKSNLY LVFEYLDTDL
110 120 130 140 150
KKFIDSHRKG SNPRPLEASL VQRFMFQLFK GVAHCHSHGV LHRDLKPQNL
160 170 180 190 200
LLDKDKGILK IADLGLSRAF TVPLKAYTHE IVTLWYRAPE VLLGSTHYST
210 220 230 240 250
AVDIWSVGCI FAEMIRRQAL FPGDSEFQQL LHIFRLLGTP TEQQWPGVMA
260 270 280 290 300
LRDWHVYPKW EPQDLSRAVP SLSPEGIDLL TQMLKYNPAE RISAKAALDH
310
PYFDSLDKSQ F
Length:311
Mass (Da):35,582
Last modified:July 10, 2007 - v2
Checksum:iA04E60AEF83E5BB0
GO
Isoform 2 (identifier: Q2V419-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     236-257: LLGTPTEQQWPGVMALRDWHVY → YLLLVFDANVYVYRNQFRTLAH
     258-311: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:257
Mass (Da):29,602
Checksum:i45178C9E1D5852B3
GO

Sequence cautioni

Q2V419: The sequence BAD95353 differs from that shown. Reason: Erroneous termination at position 60. Translated as Gln.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti231L → I in BAD95353 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_026473236 – 257LLGTP…DWHVY → YLLLVFDANVYVYRNQFRTL AH in isoform 2. CuratedAdd BLAST22
Alternative sequenceiVSP_026474258 – 311Missing in isoform 2. CuratedAdd BLAST54

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ297937 mRNA. Translation: CAC34053.1.
AC005499 Genomic DNA. Translation: AAC67356.1.
CP002685 Genomic DNA. Translation: AEC09557.1.
CP002685 Genomic DNA. Translation: AEC09558.1.
CP002685 Genomic DNA. Translation: ANM62340.1.
AK221669 mRNA. Translation: BAD95353.1. Sequence problems.
PIRiC84807.
RefSeqiNP_001031507.1. NM_001036430.3. [Q2V419-1]
NP_001324504.1. NM_001336710.1. [Q2V419-2]
NP_181396.2. NM_129419.3. [Q2V419-2]
UniGeneiAt.10323.
At.37270.

Genome annotation databases

EnsemblPlantsiAT2G38620.1; AT2G38620.1; AT2G38620. [Q2V419-2]
AT2G38620.2; AT2G38620.2; AT2G38620. [Q2V419-1]
AT2G38620.3; AT2G38620.3; AT2G38620. [Q2V419-2]
GeneIDi818444.
GrameneiAT2G38620.1; AT2G38620.1; AT2G38620.
AT2G38620.2; AT2G38620.2; AT2G38620.
AT2G38620.3; AT2G38620.3; AT2G38620.
KEGGiath:AT2G38620.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCKB12_ARATH
AccessioniPrimary (citable) accession number: Q2V419
Secondary accession number(s): Q56XK5, Q9ZVI4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: October 25, 2017
This is version 99 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families