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Q2V419

- CKB12_ARATH

UniProt

Q2V419 - CKB12_ARATH

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Protein
Cyclin-dependent kinase B1-2
Gene
CDKB1-2, At2g38620, T6A23.18
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATP By similarity
Active sitei144 – 1441Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC
  3. cyclin binding Source: TAIR
  4. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  5. protein binding Source: TAIR
  6. protein serine/threonine kinase activity Source: TAIR

GO - Biological processi

  1. regulation of cell cycle Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G38620-MONOMER.
ARA:GQT-1788-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase B1-2 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKB1;2
Gene namesi
Name:CDKB1-2
Ordered Locus Names:At2g38620
ORF Names:T6A23.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G38620.

Subcellular locationi

GO - Cellular componenti

  1. cyclin-dependent protein kinase holoenzyme complex Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 311311Cyclin-dependent kinase B1-2
PRO_0000293114Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei15 – 151Phosphotyrosine By similarity
Modified residuei178 – 1781Phosphothreonine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ2V419.
PRIDEiQ2V419.

Expressioni

Tissue specificityi

Expressed in flowers.1 Publication

Gene expression databases

GenevestigatoriQ2V419.

Interactioni

Subunit structurei

Interacts with CKS1.1 Publication

Protein-protein interaction databases

BioGridi3786. 40 interactions.
IntActiQ2V419. 9 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ2V419.
SMRiQ2V419. Positions 1-308.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 303300Protein kinase
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
KOiK07760.
OMAiCALRDWH.
PhylomeDBiQ2V419.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q2V419-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEKYEKLEKV GEGTYGKVYK AMEKTTGKLV ALKKTRLEMD EEGIPPTALR    50
EISLLQMLSQ SIYIVRLLCV EHVIQSKDST VSHSPKSNLY LVFEYLDTDL 100
KKFIDSHRKG SNPRPLEASL VQRFMFQLFK GVAHCHSHGV LHRDLKPQNL 150
LLDKDKGILK IADLGLSRAF TVPLKAYTHE IVTLWYRAPE VLLGSTHYST 200
AVDIWSVGCI FAEMIRRQAL FPGDSEFQQL LHIFRLLGTP TEQQWPGVMA 250
LRDWHVYPKW EPQDLSRAVP SLSPEGIDLL TQMLKYNPAE RISAKAALDH 300
PYFDSLDKSQ F 311
Length:311
Mass (Da):35,582
Last modified:July 10, 2007 - v2
Checksum:iA04E60AEF83E5BB0
GO
Isoform 2 (identifier: Q2V419-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     236-257: LLGTPTEQQWPGVMALRDWHVY → YLLLVFDANVYVYRNQFRTLAH
     258-311: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:257
Mass (Da):29,602
Checksum:i45178C9E1D5852B3
GO

Sequence cautioni

The sequence BAD95353.1 differs from that shown. Reason: Erroneous termination at position 60. Translated as Gln.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei236 – 25722LLGTP…DWHVY → YLLLVFDANVYVYRNQFRTL AH in isoform 2.
VSP_026473Add
BLAST
Alternative sequencei258 – 31154Missing in isoform 2.
VSP_026474Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti231 – 2311L → I in BAD95353. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ297937 mRNA. Translation: CAC34053.1.
AC005499 Genomic DNA. Translation: AAC67356.1.
CP002685 Genomic DNA. Translation: AEC09557.1.
CP002685 Genomic DNA. Translation: AEC09558.1.
AK221669 mRNA. Translation: BAD95353.1. Sequence problems.
PIRiC84807.
RefSeqiNP_001031507.1. NM_001036430.2. [Q2V419-1]
NP_181396.2. NM_129419.3. [Q2V419-2]
UniGeneiAt.10323.
At.37270.

Genome annotation databases

EnsemblPlantsiAT2G38620.2; AT2G38620.2; AT2G38620. [Q2V419-1]
GeneIDi818444.
KEGGiath:AT2G38620.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ297937 mRNA. Translation: CAC34053.1 .
AC005499 Genomic DNA. Translation: AAC67356.1 .
CP002685 Genomic DNA. Translation: AEC09557.1 .
CP002685 Genomic DNA. Translation: AEC09558.1 .
AK221669 mRNA. Translation: BAD95353.1 . Sequence problems.
PIRi C84807.
RefSeqi NP_001031507.1. NM_001036430.2. [Q2V419-1 ]
NP_181396.2. NM_129419.3. [Q2V419-2 ]
UniGenei At.10323.
At.37270.

3D structure databases

ProteinModelPortali Q2V419.
SMRi Q2V419. Positions 1-308.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 3786. 40 interactions.
IntActi Q2V419. 9 interactions.

Proteomic databases

PaxDbi Q2V419.
PRIDEi Q2V419.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G38620.2 ; AT2G38620.2 ; AT2G38620 . [Q2V419-1 ]
GeneIDi 818444.
KEGGi ath:AT2G38620.

Organism-specific databases

GeneFarmi 2965. 107.
TAIRi AT2G38620.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
KOi K07760.
OMAi CALRDWH.
PhylomeDBi Q2V419.

Enzyme and pathway databases

BioCyci ARA:AT2G38620-MONOMER.
ARA:GQT-1788-MONOMER.

Gene expression databases

Genevestigatori Q2V419.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of novel cyclin-dependent kinases interacting with the CKS1 protein of Arabidopsis."
    Boudolf V., Rombauts S., Naudts M., Inze D., de Veylder L.
    J. Exp. Bot. 52:1381-1382(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH CKS1.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Genome-wide analysis of core cell cycle genes in Arabidopsis."
    Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.
    Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Cell cycle regulation in plant development."
    Inze D., de Veylder L.
    Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiCKB12_ARATH
AccessioniPrimary (citable) accession number: Q2V419
Secondary accession number(s): Q56XK5, Q9ZVI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: June 11, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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