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Q2UY11 (COSA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Collagen alpha-1(XXVIII) chain
Gene names
Name:Col28a1
Synonyms:Col28
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1141 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May act as a cell-binding protein.

Subunit structure

Trimer or homomer. Secreted into as a 135 kDa monomer under reducing conditions and as a homotrimer under non-reducing conditions. Ref.1

Subcellular location

Secretedextracellular spaceextracellular matrixbasement membrane Ref.1.

Tissue specificity

Expressed in skin, intestine, sternum, brain and kidney. Lower expression is also observed in heart, lung, sciatic nerve, dorsal root ganglia, peripheral nerves and calvaria of newborn mice and in intestine and brain of adult mice. Found in basement membrane surrounding a particular subset of Schwann cells in adult sciatic nerve. Ref.1

Developmental stage

Major expression in dorsal root ganglia and peripheral nerves, with small amounts in connective tissues like calvaria and skin. Ref.1

Sequence similarities

Belongs to the VWA-containing collagen family.

Contains 1 BPTI/Kunitz inhibitor domain.

Contains 5 collagen-like domains.

Contains 2 VWFA domains.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentBasement membrane
Extracellular matrix
Secreted
   Coding sequence diversityAlternative splicing
   DomainCollagen
Repeat
Signal
   Molecular functionProtease inhibitor
Serine protease inhibitor
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell adhesion

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentbasement membrane

Inferred from direct assay Ref.1. Source: MGI

collagen

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionserine-type endopeptidase inhibitor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q2UY11-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q2UY11-2)

The sequence of this isoform differs from the canonical sequence as follows:
     667-699: GEPGVRGPPGPSGPRGIGTQGPKGDTGQKGLPG → VRFLKEAKILVFKKVLIDDFGKCVLFLSGTQEE
     700-1141: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 11411121Collagen alpha-1(XXVIII) chain
PRO_5000074665

Regions

Domain48 – 227180VWFA 1
Domain243 – 30058Collagen-like 1
Domain301 – 35858Collagen-like 2
Domain501 – 54444Collagen-like 3
Domain545 – 58844Collagen-like 4
Domain733 – 76937Collagen-like 5
Domain798 – 976179VWFA 2
Domain1088 – 113851BPTI/Kunitz inhibitor

Amino acid modifications

Disulfide bond1088 ↔ 1138 By similarity
Disulfide bond1097 ↔ 1121 By similarity
Disulfide bond1113 ↔ 1134 By similarity

Natural variations

Alternative sequence667 – 69933GEPGV…KGLPG → VRFLKEAKILVFKKVLIDDF GKCVLFLSGTQEE in isoform 2.
VSP_031095
Alternative sequence700 – 1141442Missing in isoform 2.
VSP_031096

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: A2C43C4BB7913272

FASTA1,141118,749
        10         20         30         40         50         60 
MRRRDVAFCL LLLPAFMTQA VYGQRKKGPK PNTLARKNDF QDAICFIDVV FILDSSESSK 

        70         80         90        100        110        120 
IVLFDNQKDF VDSLSEKIFQ LTPGRSLKYD IKLAALQFSS SVQIDPPLSS WKDLRTFKQR 

       130        140        150        160        170        180 
VKSLNLIGQG TFSYYAISNA TRLLKREGRK DGVKVALLMT DGIDHPKSPD VQSISEDARI 

       190        200        210        220        230        240 
LGISFITVGL STVVNEAKLR LISGDPSNEP VLLLSDPTLV DRIQERLGVL FERKCEHKIC 

       250        260        270        280        290        300 
ECEKGEPGDP GPPGTHGNPG IKGERGPKGN PGDAQKGETG ERGPVGIPGY KGDKGERGEC 

       310        320        330        340        350        360 
GKPGMKGDKG PEGPYGPKGP RGIQGIGGPP GDPGPKGFQG NKGEPGPPGP YGPPGAPGIG 

       370        380        390        400        410        420 
QQGVKGERGQ EGRMGAPGPI GIGEPGQPGP RGPEGAPGER GLPGEGFPGP KGEKGSEGPI 

       430        440        450        460        470        480 
GPQGLQGLSI KGDKGDLGPV GPQGPAGIPG IGSQGEQGIQ GPSGPPGPQG PPGQGSPGPK 

       490        500        510        520        530        540 
GEVGQMGPTG PRGPMGIGVQ GPKGEPGTVG LPGQPGVPGE DGASGKKGEA GLPGTRGPEG 

       550        560        570        580        590        600 
MPGKGQPGPK GDEGKKGSKG NQGQRGFPGP EGPKGEPGVM GPFGMPGASI PGPSGPKGDR 

       610        620        630        640        650        660 
GGPGMPGLKG EPGLPVRGPK GAQGPRGPVG APGLKGDGYP GVAGPRGLPG PPGPMGLRGV 

       670        680        690        700        710        720 
GDTGAKGEPG VRGPPGPSGP RGIGTQGPKG DTGQKGLPGP PGPPGYGSQG IKGEQGPQGF 

       730        740        750        760        770        780 
PGSKGTVGLG LPGQKGEHGD RGDVGRKGEK GETGEPGSPG KQGLQGPKGD LGLTKEEIIK 

       790        800        810        820        830        840 
LIIEICGCGP KCKETPLELV FVIDSSESVG PENFQIIQSF VKTLADRVAL DLGTARIGII 

       850        860        870        880        890        900 
NYSHKVEKVA SLKQFSSKDD FKLVVDNMQY LGEGTYTATA LQAANDMFKE ARPGVKKVAL 

       910        920        930        940        950        960 
VITDGQTDSR DKKKLADVVK DANDSNVEIF VIGVVKKDDP NFEIFHKEMN LIATDAEHVY 

       970        980        990       1000       1010       1020 
QFDDFFTLQD TLKQKLSKKI CEDFDSYLIQ VFGSPSFQPE FGVSEREVSV STPKPAKEMS 

      1030       1040       1050       1060       1070       1080 
KSFNVSRGQN EETESYVLTE AGILAIPTPP EATNTLEPLL SSREGVETRT PNPNLLQSEK 

      1090       1100       1110       1120       1130       1140 
SLYKDPRCEE ALKPGECGDY VVRWYYDKQV NSCARFWFSG CNGSGNRFHS EKECRETCIK 


Q 

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Isoform 2 [UniParc].

Checksum: 35385C4AE8FC3DC6
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FASTA69971,075

References

[1]"Collagen XXVIII, a novel von Willebrand factor A domain-containing protein with many imperfections in the collagenous domain."
Veit G., Kobbe B., Keene D.R., Paulsson M., Koch M., Wagener R.
J. Biol. Chem. 281:3494-3504(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBUNITS, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: C57BL/6J.
Tissue: Brain and Limb.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ890449 mRNA. Translation: CAI67593.1.
AJ890450 mRNA. Translation: CAI67594.1.
RefSeqNP_001032954.1. NM_001037865.1.
XP_006505094.1. XM_006505031.1.
XP_006505095.1. XM_006505032.1.
UniGeneMm.297404.

3D structure databases

ProteinModelPortalQ2UY11.
SMRQ2UY11. Positions 46-232, 791-1028, 1094-1139.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ2UY11.

Proteomic databases

PRIDEQ2UY11.

Protocols and materials databases

DNASU213945.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000115537; ENSMUSP00000111199; ENSMUSG00000068794. [Q2UY11-1]
GeneID213945.
KEGGmmu:213945.
UCSCuc009axi.1. mouse. [Q2UY11-1]

Organism-specific databases

CTD340267.
MGIMGI:2685312. Col28a1.

Phylogenomic databases

eggNOGNOG236064.
GeneTreeENSGT00730000110647.
HOGENOMHOG000085654.
HOVERGENHBG104822.
InParanoidQ2UY11.
OMAQKDFVDS.
OrthoDBEOG7NSB1W.
PhylomeDBQ2UY11.
TreeFamTF331207.

Gene expression databases

BgeeQ2UY11.
CleanExMM_COL28A1.
GenevestigatorQ2UY11.

Family and domain databases

Gene3D3.40.50.410. 2 hits.
4.10.410.10. 1 hit.
InterProIPR008160. Collagen.
IPR002223. Prot_inh_Kunz-m.
IPR020901. Prtase_inh_Kunz-CS.
IPR002035. VWF_A.
[Graphical view]
PfamPF01391. Collagen. 3 hits.
PF00014. Kunitz_BPTI. 1 hit.
PF00092. VWA. 2 hits.
[Graphical view]
SMARTSM00131. KU. 1 hit.
SM00327. VWA. 2 hits.
[Graphical view]
SUPFAMSSF53300. SSF53300. 2 hits.
SSF57362. SSF57362. 1 hit.
PROSITEPS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.
PS50234. VWFA. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio374132.
PROQ2UY11.
SOURCESearch...

Entry information

Entry nameCOSA1_MOUSE
AccessionPrimary (citable) accession number: Q2UY11
Secondary accession number(s): Q2UY10
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 24, 2006
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot