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Protein

Collagen alpha-1(XXVIII) chain

Gene

Col28a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May act as a cell-binding protein.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-1650814. Collagen biosynthesis and modifying enzymes.

Names & Taxonomyi

Protein namesi
Recommended name:
Collagen alpha-1(XXVIII) chain
Gene namesi
Name:Col28a1
Synonyms:Col28
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2685312. Col28a1.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: MGI
  • collagen trimer Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_500007466521 – 1141Collagen alpha-1(XXVIII) chainAdd BLAST1121

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi1088 ↔ 1138PROSITE-ProRule annotation
Disulfide bondi1097 ↔ 1121PROSITE-ProRule annotation
Disulfide bondi1113 ↔ 1134PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ2UY11.
PaxDbiQ2UY11.
PRIDEiQ2UY11.

PTM databases

PhosphoSitePlusiQ2UY11.

Expressioni

Tissue specificityi

Expressed in skin, intestine, sternum, brain and kidney. Lower expression is also observed in heart, lung, sciatic nerve, dorsal root ganglia, peripheral nerves and calvaria of newborn mice and in intestine and brain of adult mice. Found in basement membrane surrounding a particular subset of Schwann cells in adult sciatic nerve.1 Publication

Developmental stagei

Major expression in dorsal root ganglia and peripheral nerves, with small amounts in connective tissues like calvaria and skin.1 Publication

Gene expression databases

BgeeiENSMUSG00000068794.
CleanExiMM_COL28A1.

Interactioni

Subunit structurei

Trimer or homomer. Secreted into as a 135 kDa monomer under reducing conditions and as a homotrimer under non-reducing conditions.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000111199.

Structurei

3D structure databases

ProteinModelPortaliQ2UY11.
SMRiQ2UY11.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 227VWFA 1PROSITE-ProRule annotationAdd BLAST180
Domaini243 – 300Collagen-like 1Add BLAST58
Domaini301 – 358Collagen-like 2Add BLAST58
Domaini501 – 544Collagen-like 3Add BLAST44
Domaini545 – 588Collagen-like 4Add BLAST44
Domaini733 – 769Collagen-like 5Add BLAST37
Domaini798 – 976VWFA 2PROSITE-ProRule annotationAdd BLAST179
Domaini1088 – 1138BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd BLAST51

Sequence similaritiesi

Belongs to the VWA-containing collagen family.Curated
Contains 1 BPTI/Kunitz inhibitor domain.PROSITE-ProRule annotation
Contains 5 collagen-like domains.Curated
Contains 2 VWFA domains.PROSITE-ProRule annotation

Keywords - Domaini

Collagen, Repeat, Signal

Phylogenomic databases

eggNOGiKOG1217. Eukaryota.
KOG3544. Eukaryota.
ENOG410ZJ4P. LUCA.
GeneTreeiENSGT00820000126981.
HOGENOMiHOG000085654.
HOVERGENiHBG104822.
InParanoidiQ2UY11.
OMAiEKECQET.
OrthoDBiEOG091G0T4R.
PhylomeDBiQ2UY11.
TreeFamiTF331207.

Family and domain databases

Gene3Di3.40.50.410. 2 hits.
4.10.410.10. 1 hit.
InterProiIPR008160. Collagen.
IPR002223. Kunitz_BPTI.
IPR020901. Prtase_inh_Kunz-CS.
IPR002035. VWF_A.
[Graphical view]
PfamiPF01391. Collagen. 1 hit.
PF00014. Kunitz_BPTI. 1 hit.
PF00092. VWA. 2 hits.
[Graphical view]
SMARTiSM00131. KU. 1 hit.
SM00327. VWA. 2 hits.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 2 hits.
SSF57362. SSF57362. 1 hit.
PROSITEiPS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.
PS50234. VWFA. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2UY11-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRRDVAFCL LLLPAFMTQA VYGQRKKGPK PNTLARKNDF QDAICFIDVV
60 70 80 90 100
FILDSSESSK IVLFDNQKDF VDSLSEKIFQ LTPGRSLKYD IKLAALQFSS
110 120 130 140 150
SVQIDPPLSS WKDLRTFKQR VKSLNLIGQG TFSYYAISNA TRLLKREGRK
160 170 180 190 200
DGVKVALLMT DGIDHPKSPD VQSISEDARI LGISFITVGL STVVNEAKLR
210 220 230 240 250
LISGDPSNEP VLLLSDPTLV DRIQERLGVL FERKCEHKIC ECEKGEPGDP
260 270 280 290 300
GPPGTHGNPG IKGERGPKGN PGDAQKGETG ERGPVGIPGY KGDKGERGEC
310 320 330 340 350
GKPGMKGDKG PEGPYGPKGP RGIQGIGGPP GDPGPKGFQG NKGEPGPPGP
360 370 380 390 400
YGPPGAPGIG QQGVKGERGQ EGRMGAPGPI GIGEPGQPGP RGPEGAPGER
410 420 430 440 450
GLPGEGFPGP KGEKGSEGPI GPQGLQGLSI KGDKGDLGPV GPQGPAGIPG
460 470 480 490 500
IGSQGEQGIQ GPSGPPGPQG PPGQGSPGPK GEVGQMGPTG PRGPMGIGVQ
510 520 530 540 550
GPKGEPGTVG LPGQPGVPGE DGASGKKGEA GLPGTRGPEG MPGKGQPGPK
560 570 580 590 600
GDEGKKGSKG NQGQRGFPGP EGPKGEPGVM GPFGMPGASI PGPSGPKGDR
610 620 630 640 650
GGPGMPGLKG EPGLPVRGPK GAQGPRGPVG APGLKGDGYP GVAGPRGLPG
660 670 680 690 700
PPGPMGLRGV GDTGAKGEPG VRGPPGPSGP RGIGTQGPKG DTGQKGLPGP
710 720 730 740 750
PGPPGYGSQG IKGEQGPQGF PGSKGTVGLG LPGQKGEHGD RGDVGRKGEK
760 770 780 790 800
GETGEPGSPG KQGLQGPKGD LGLTKEEIIK LIIEICGCGP KCKETPLELV
810 820 830 840 850
FVIDSSESVG PENFQIIQSF VKTLADRVAL DLGTARIGII NYSHKVEKVA
860 870 880 890 900
SLKQFSSKDD FKLVVDNMQY LGEGTYTATA LQAANDMFKE ARPGVKKVAL
910 920 930 940 950
VITDGQTDSR DKKKLADVVK DANDSNVEIF VIGVVKKDDP NFEIFHKEMN
960 970 980 990 1000
LIATDAEHVY QFDDFFTLQD TLKQKLSKKI CEDFDSYLIQ VFGSPSFQPE
1010 1020 1030 1040 1050
FGVSEREVSV STPKPAKEMS KSFNVSRGQN EETESYVLTE AGILAIPTPP
1060 1070 1080 1090 1100
EATNTLEPLL SSREGVETRT PNPNLLQSEK SLYKDPRCEE ALKPGECGDY
1110 1120 1130 1140
VVRWYYDKQV NSCARFWFSG CNGSGNRFHS EKECRETCIK Q
Length:1,141
Mass (Da):118,749
Last modified:January 24, 2006 - v1
Checksum:iA2C43C4BB7913272
GO
Isoform 2 (identifier: Q2UY11-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     667-699: GEPGVRGPPGPSGPRGIGTQGPKGDTGQKGLPG → VRFLKEAKILVFKKVLIDDFGKCVLFLSGTQEE
     700-1141: Missing.

Show »
Length:699
Mass (Da):71,075
Checksum:i35385C4AE8FC3DC6
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_031095667 – 699GEPGV…KGLPG → VRFLKEAKILVFKKVLIDDF GKCVLFLSGTQEE in isoform 2. 1 PublicationAdd BLAST33
Alternative sequenceiVSP_031096700 – 1141Missing in isoform 2. 1 PublicationAdd BLAST442

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ890449 mRNA. Translation: CAI67593.1.
AJ890450 mRNA. Translation: CAI67594.1.
CCDSiCCDS39424.1. [Q2UY11-1]
RefSeqiNP_001032954.1. NM_001037865.1. [Q2UY11-1]
XP_006505094.1. XM_006505031.2. [Q2UY11-1]
XP_006505095.1. XM_006505032.3. [Q2UY11-1]
XP_017176991.1. XM_017321502.1. [Q2UY11-1]
UniGeneiMm.297404.

Genome annotation databases

EnsembliENSMUST00000115537; ENSMUSP00000111199; ENSMUSG00000068794. [Q2UY11-1]
GeneIDi213945.
KEGGimmu:213945.
UCSCiuc009axi.1. mouse. [Q2UY11-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ890449 mRNA. Translation: CAI67593.1.
AJ890450 mRNA. Translation: CAI67594.1.
CCDSiCCDS39424.1. [Q2UY11-1]
RefSeqiNP_001032954.1. NM_001037865.1. [Q2UY11-1]
XP_006505094.1. XM_006505031.2. [Q2UY11-1]
XP_006505095.1. XM_006505032.3. [Q2UY11-1]
XP_017176991.1. XM_017321502.1. [Q2UY11-1]
UniGeneiMm.297404.

3D structure databases

ProteinModelPortaliQ2UY11.
SMRiQ2UY11.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000111199.

PTM databases

PhosphoSitePlusiQ2UY11.

Proteomic databases

MaxQBiQ2UY11.
PaxDbiQ2UY11.
PRIDEiQ2UY11.

Protocols and materials databases

DNASUi213945.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000115537; ENSMUSP00000111199; ENSMUSG00000068794. [Q2UY11-1]
GeneIDi213945.
KEGGimmu:213945.
UCSCiuc009axi.1. mouse. [Q2UY11-1]

Organism-specific databases

CTDi340267.
MGIiMGI:2685312. Col28a1.

Phylogenomic databases

eggNOGiKOG1217. Eukaryota.
KOG3544. Eukaryota.
ENOG410ZJ4P. LUCA.
GeneTreeiENSGT00820000126981.
HOGENOMiHOG000085654.
HOVERGENiHBG104822.
InParanoidiQ2UY11.
OMAiEKECQET.
OrthoDBiEOG091G0T4R.
PhylomeDBiQ2UY11.
TreeFamiTF331207.

Enzyme and pathway databases

ReactomeiR-MMU-1650814. Collagen biosynthesis and modifying enzymes.

Miscellaneous databases

PROiQ2UY11.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000068794.
CleanExiMM_COL28A1.

Family and domain databases

Gene3Di3.40.50.410. 2 hits.
4.10.410.10. 1 hit.
InterProiIPR008160. Collagen.
IPR002223. Kunitz_BPTI.
IPR020901. Prtase_inh_Kunz-CS.
IPR002035. VWF_A.
[Graphical view]
PfamiPF01391. Collagen. 1 hit.
PF00014. Kunitz_BPTI. 1 hit.
PF00092. VWA. 2 hits.
[Graphical view]
SMARTiSM00131. KU. 1 hit.
SM00327. VWA. 2 hits.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 2 hits.
SSF57362. SSF57362. 1 hit.
PROSITEiPS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.
PS50234. VWFA. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOSA1_MOUSE
AccessioniPrimary (citable) accession number: Q2UY11
Secondary accession number(s): Q2UY10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 24, 2006
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.