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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trm8

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei213S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei216UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trm8
ORF Names:AO090009000430
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006564 Componenti: Chromosome 1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705881 – 330tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST330

Interactioni

Subunit structurei

Forms a complex with trm82.UniRule annotation

Protein-protein interaction databases

STRINGi5062.CADAORAP00003816

Structurei

3D structure databases

ProteinModelPortaliQ2UU72
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni118 – 119S-adenosyl-L-methionine bindingUniRule annotation2
Regioni193 – 194S-adenosyl-L-methionine bindingUniRule annotation2
Regioni302 – 304S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000260965
KOiK03439
OMAiIGMEIRV
OrthoDBiEOG092C3FCG

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 2 hits
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q2UU72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPPAKRQK RDQYRKRAAA AANEDTGKVK LPQKKFYRQR AHANPFSDHQ
60 70 80 90 100
LDYPLSPAHM DWSSHYPAFV NPDPEQKNLA GARKLLKDVE VVDIGCGFGG
110 120 130 140 150
LLVGLAPLLP ETLMLGMEIR TQVIEYVENR IQALRTQQNQ LKNSSTTASE
160 170 180 190 200
SPAPAIPAEP ATDGASPDAA STPETSNSPV PGGYQNISAL RSNTMKFFPN
210 220 230 240 250
FFGKQQLSKI FICFPDPHFK AKKHKARIIS ENLNAEYAYA LKPGGLLYTI
260 270 280 290 300
TDVEEYHHWV LRHFREEGEH EASEGGVKDL FERVSEEELA SDPCVEVMRE
310 320 330
STEEGKKVTR NKGNKYVAVF RRKADPEWPA
Length:330
Mass (Da):36,964
Last modified:January 24, 2006 - v1
Checksum:i28C5FFDA22D155BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007150 Genomic DNA Translation: BAE54893.1
RefSeqiXP_001816895.1, XM_001816843.1

Genome annotation databases

EnsemblFungiiBAE54893; BAE54893; AO090009000430
GeneIDi5988825
KEGGiaor:AO090009000430

Similar proteinsi

Entry informationi

Entry nameiTRMB_ASPOR
AccessioniPrimary (citable) accession number: Q2UU72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: January 24, 2006
Last modified: April 25, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health