Q2UPW4 (DAPB_ASPOR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable dipeptidyl-aminopeptidase B
Short name=DPAP B
EC=3.4.14.5

Gene names

Name:	dapB
ORF Names:	AO090005001482

Organism

Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) [Complete proteome]

Taxonomic identifier

510516 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus oryzae

Protein attributes

Sequence length	902 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Type IV dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline By similarity.
Catalytic activity	Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Subcellular location	Vacuole membrane; Single-pass type II membrane protein By similarity. Note: Lysosome-like vacuoles By similarity.
Sequence similarities	Belongs to the peptidase S9B family.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Aminopeptidase Hydrolase Protease Serine protease
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 902	902	Probable dipeptidyl-aminopeptidase B		PRO_0000412138
Regions
	Topological domain	1 – 78	78	Cytoplasmic Potential
	Transmembrane	79 – 99	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	100 – 902	803	Vacuolar Potential
Sites
	Active site	740	1	Charge relay system By similarity
	Active site	817	1	Charge relay system By similarity
	Active site	850	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	335	1	N-linked (GlcNAc...) Potential
	Glycosylation	626	1	N-linked (GlcNAc...) Potential
	Glycosylation	794	1	N-linked (GlcNAc...) Potential
	Glycosylation	799	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2UPW4 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 682AE97AABC5F565	FASTA	902	101,664
	10 20 30 40 50 60 MTRRRSTSGT SSRSSTDSGL SVDTAYLEDN KHNNFANGTS GLTDETKYRD VEDAEADVDE 70 80 90 100 110 120 PFLPTSSKKL GSGSRTRQIF WALVILCLGG WVLALVLFLT HGRASSQTAS ETLQQQESDS 130 140 150 160 170 180 GSTSAGRPVT LQQVLTGSWN PRAHAISWIA GPDGEDGLLV QRAEVDKEGY MRVDDIRSQE 190 200 210 220 230 240 GDDVDSQSGR ILIDKAAVRV NGETLMPTFT WPSPDLNKVL LMSNHEKNWR YSFTGRYWIF 250 260 270 280 290 300 DVATQTAQPL DPSVPDGRVQ LALWSPSSDA VVFVRDNNMY LRKLSSESVV SITKDGGEDL 310 320 330 340 350 360 FYGIPDWVYE EEVITDKSVT WWSNDGKYVA FLRTNESAVP EFPVQYFVSR PSGKRPPPGL 370 380 390 400 410 420 ENYPEVRQIK YPKAGSPNPV VNLLFYDVEK DEVFPVDVPD DFPDDDRIII EVLWASEGKV 430 440 450 460 470 480 IVRATNRESD RVKVFLIDTK SRTGKLVRFE DIANLDGGWV EPSHYTKFIP ADPSNGRPDD 490 500 510 520 530 540 GYIDTVIHDG YDHLAYFTPL DNPDPIMLTT GEWEVVEAPS AVDLRRGIVY FVATKESPTQ 550 560 570 580 590 600 RHVYRVHLDG SNLQALTDTS KPGFYDVSFS DGAGYALLSY NGPSVPWQAI INTGGDEITF 610 620 630 640 650 660 EKTIEKNPRL ASMVETYALP TEIYQNVTID GFTLQLVERR PPHFNPAKKY PVVFQLYNGP 670 680 690 700 710 720 TSQRVDRKFT IDFQSYIASN LGYIVVTLDA RGTGYSGRKV RCAVRGNLGH YEAHDQITTA 730 740 750 760 770 780 KMWAKKPYVD ETRMAIWGWS YGGFMTLKVL EQDAGETFQY GMAVAPVTDW RFYDSVYTER 790 800 810 820 830 840 YMHTPEHNPS GYENSTITNV SALSKATRFL LIHGASDDNV HIQNTLTFVD KLDLLNVQNY 850 860 870 880 890 900 DMHFYPDSDH NIYFHNAHFM IYERLSNWLI NAFNGEWHQI ANPVPEDSIW DSVKRSVPAF AH

References

[1]

"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H. Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP007151 Genomic DNA. Translation: BAE56401.1.
3D structure databases
ProteinModelPortal	Q2UPW4.
ModBase	Search...
Protein-protein interaction databases
STRING	5062.CADAORAP00003299.
Protein family/group databases
MEROPS	S09.006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAORAT00003356; CADAORAP00003299; CADAORAG00003356.
Phylogenomic databases
HOGENOM	HOG000189891.
OrthoDB	EOG4Q885J.
Family and domain databases
InterPro	IPR001375. Peptidase_S9. IPR002469. Peptidase_S9B. [Graphical view]
Pfam	PF00930. DPPIV_N. 1 hit. PF00326. Peptidase_S9. 1 hit. [Graphical view]
PROSITE	PS00708. PRO_ENDOPEP_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPB_ASPOR

Accession

Primary (citable) accession number: Q2UPW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	January 24, 2006
Last modified:	April 3, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program