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Protein

Probable dipeptidyl-aminopeptidase B

Gene

dapB

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Type IV dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.By similarity

Catalytic activityi

Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei740 – 7401Charge relay systemBy similarity
Active sitei817 – 8171Charge relay systemBy similarity
Active sitei850 – 8501Charge relay systemBy similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. serine-type peptidase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS09.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dipeptidyl-aminopeptidase B (EC:3.4.14.5)
Short name:
DPAP B
Gene namesi
Name:dapB
ORF Names:AO090005001482
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006564 Componenti: Chromosome 1

Subcellular locationi

Vacuole membrane By similarity; Single-pass type II membrane protein By similarity
Note: Lysosome-like vacuoles.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7878CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei79 – 9921Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini100 – 902803VacuolarSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 902902Probable dipeptidyl-aminopeptidase BPRO_0000412138Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi335 – 3351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi626 – 6261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi794 – 7941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi799 – 7991N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi5062.CADAORAP00003299.

Structurei

3D structure databases

ProteinModelPortaliQ2UPW4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9B family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000189891.
OrthoDBiEOG72VHFG.

Family and domain databases

Gene3Di2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B.
[Graphical view]
PfamiPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2UPW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRRRSTSGT SSRSSTDSGL SVDTAYLEDN KHNNFANGTS GLTDETKYRD
60 70 80 90 100
VEDAEADVDE PFLPTSSKKL GSGSRTRQIF WALVILCLGG WVLALVLFLT
110 120 130 140 150
HGRASSQTAS ETLQQQESDS GSTSAGRPVT LQQVLTGSWN PRAHAISWIA
160 170 180 190 200
GPDGEDGLLV QRAEVDKEGY MRVDDIRSQE GDDVDSQSGR ILIDKAAVRV
210 220 230 240 250
NGETLMPTFT WPSPDLNKVL LMSNHEKNWR YSFTGRYWIF DVATQTAQPL
260 270 280 290 300
DPSVPDGRVQ LALWSPSSDA VVFVRDNNMY LRKLSSESVV SITKDGGEDL
310 320 330 340 350
FYGIPDWVYE EEVITDKSVT WWSNDGKYVA FLRTNESAVP EFPVQYFVSR
360 370 380 390 400
PSGKRPPPGL ENYPEVRQIK YPKAGSPNPV VNLLFYDVEK DEVFPVDVPD
410 420 430 440 450
DFPDDDRIII EVLWASEGKV IVRATNRESD RVKVFLIDTK SRTGKLVRFE
460 470 480 490 500
DIANLDGGWV EPSHYTKFIP ADPSNGRPDD GYIDTVIHDG YDHLAYFTPL
510 520 530 540 550
DNPDPIMLTT GEWEVVEAPS AVDLRRGIVY FVATKESPTQ RHVYRVHLDG
560 570 580 590 600
SNLQALTDTS KPGFYDVSFS DGAGYALLSY NGPSVPWQAI INTGGDEITF
610 620 630 640 650
EKTIEKNPRL ASMVETYALP TEIYQNVTID GFTLQLVERR PPHFNPAKKY
660 670 680 690 700
PVVFQLYNGP TSQRVDRKFT IDFQSYIASN LGYIVVTLDA RGTGYSGRKV
710 720 730 740 750
RCAVRGNLGH YEAHDQITTA KMWAKKPYVD ETRMAIWGWS YGGFMTLKVL
760 770 780 790 800
EQDAGETFQY GMAVAPVTDW RFYDSVYTER YMHTPEHNPS GYENSTITNV
810 820 830 840 850
SALSKATRFL LIHGASDDNV HIQNTLTFVD KLDLLNVQNY DMHFYPDSDH
860 870 880 890 900
NIYFHNAHFM IYERLSNWLI NAFNGEWHQI ANPVPEDSIW DSVKRSVPAF

AH
Length:902
Mass (Da):101,664
Last modified:January 23, 2006 - v1
Checksum:i682AE97AABC5F565
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007151 Genomic DNA. Translation: BAE56401.1.

Genome annotation databases

EnsemblFungiiCADAORAT00003356; CADAORAP00003299; CADAORAG00003356.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007151 Genomic DNA. Translation: BAE56401.1.

3D structure databases

ProteinModelPortaliQ2UPW4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5062.CADAORAP00003299.

Protein family/group databases

MEROPSiS09.006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAORAT00003356; CADAORAP00003299; CADAORAG00003356.

Phylogenomic databases

HOGENOMiHOG000189891.
OrthoDBiEOG72VHFG.

Family and domain databases

Gene3Di2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B.
[Graphical view]
PfamiPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequencing and analysis of Aspergillus oryzae."
    Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
    , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
    Nature 438:1157-1161(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 42149 / RIB 40.

Entry informationi

Entry nameiDAPB_ASPOR
AccessioniPrimary (citable) accession number: Q2UPW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2011
Last sequence update: January 23, 2006
Last modified: January 6, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.