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Protein

Probable endo-beta-1,4-glucanase B

Gene

eglB

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates (By similarity).By similarity

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei160Proton donorBy similarity1
Active sitei267NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endo-beta-1,4-glucanase B (EC:3.2.1.4)
Short name:
Endoglucanase B
Alternative name(s):
Carboxymethylcellulase B
Cellulase B
Gene namesi
Name:eglB
Synonyms:celE
ORF Names:AO090005001553
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006564 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000039405718 – 333Probable endo-beta-1,4-glucanase BAdd BLAST316

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi37N-linked (GlcNAc...)Sequence analysis1
Glycosylationi100N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ2UPQ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000111120.
KOiK01179.
OMAiFWQNLAG.
OrthoDBiEOG092C4BA5.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2UPQ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFRNLFFAA VAGSAVAAPL AKEQKKRDSV FQWIGANESG AEFGENNLPG
60 70 80 90 100
VWGTDYIFPD VSAITTLIDK GMNIFRIQFK MERLVPDSMT GAYDEAYLQN
110 120 130 140 150
LTTVVNAVTD AGVHAILDPH NYGRFNGEIM STPSDFQTFW KNLAGQFQSN
160 170 180 190 200
SLVIFDTNNE YHDMDQELVL NLNQAAIDGI REAGATEQYI FVEGNSYTGA
210 220 230 240 250
WTWTDVNDNM KNLEDPQDKI VYQMHQYLDS DGSGTSETCV SGTIGQERVT
260 270 280 290 300
SATQWLKDNK KVGIIGEFAG GNNDQCKTAV KGMLDYLAEN TDVWKGALWW
310 320 330
AAGPWWGDYM YSLEPPNGVA FTGMLDVLQA YLG
Length:333
Mass (Da):36,958
Last modified:January 24, 2006 - v1
Checksum:iEA1271CB19A55C7A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145G → E in BAD72778 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB195229 Genomic DNA. Translation: BAD72778.1.
AP007151 Genomic DNA. Translation: BAE56461.1.
RefSeqiXP_001818463.1. XM_001818411.1.

Genome annotation databases

EnsemblFungiiBAE56461; BAE56461; AO090005001553.
GeneIDi5990408.
KEGGiaor:AOR_1_2698174.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB195229 Genomic DNA. Translation: BAD72778.1.
AP007151 Genomic DNA. Translation: BAE56461.1.
RefSeqiXP_001818463.1. XM_001818411.1.

3D structure databases

ProteinModelPortaliQ2UPQ4.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAE56461; BAE56461; AO090005001553.
GeneIDi5990408.
KEGGiaor:AOR_1_2698174.

Phylogenomic databases

HOGENOMiHOG000111120.
KOiK01179.
OMAiFWQNLAG.
OrthoDBiEOG092C4BA5.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEGLB_ASPOR
AccessioniPrimary (citable) accession number: Q2UPQ4
Secondary accession number(s): Q5TKT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: January 24, 2006
Last modified: November 30, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.