Reviewed, UniProtKB/Swiss-Prot Q2UPP1 (KMO_ASPOR)

Names and origin

Protein names

Recommended name:
    Kynurenine 3-monooxygenase
    EC=1.14.13.9
Alternative name(s):
    Kynurenine 3-hydroxylase
    Biosynthesis of nicotinic acid protein 4

Gene names

Name:	bna4
ORF Names:	AO090005001567

Organism

Aspergillus oryzae [Complete proteome]

Taxonomic identifier

5062 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid By similarity.
Catalytic activity	L-kynurenine + NADPH + O2 = 3-hydroxy-L-kynurenine + NADP+ + H2O.
Cofactor	FAD By similarity.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the aromatic-ring hydroxylase family. KMO subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Mitochondrion
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kynurenine 3-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Kynurenine 3-monooxygenase		PRO_0000361922

Sequences

	Sequence		Length	Mass (Da)
	Q2UPP1-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: A1BDB232E4BDBCDB	FASTA	510	57,017
	10 20 30 40 50 60 MAQEPPRKQK VVIVGAGPVG SLAALYAAAR GDDVEVYELR GDLRDPTTIP LNFTKSINLA 70 80 90 100 110 120 LSERGISSLK GSNRDGMIEK ILNDAIPMHG RMIHGRDDGK LWEAAQAYDV HGQAINSVDR 130 140 150 160 170 180 STLNNALLDE LERTPNVKLF FNHKLTGADF QSNRAWFERR APGDTPLPGS SNRVPEIEVS 190 200 210 220 230 240 FDYMIGADGA HSASRYHMMK YSRVDYQQEY IDTLWCEFRI PPSDTGDFRI SPNHLHIWPG 250 260 270 280 290 300 KEFMFIALPS PDKSFTCTLF APAAHYAQLE SSPQKLFLSF DANFPGVSPD LITPEDLQEQ 310 320 330 340 350 360 FKENPHLPLI SIKAKPHHYG SNIVIVGDAA HAILPFYGQG LNAGLEDIRV LFDFLDKHDA 370 380 390 400 410 420 FDLNASLTAR RESRRAAFQA YTDQRTADAH AINDLSKQNY LEMRWGVKTP LYKIRKSIEE 430 440 450 460 470 480 ALDLYVPSLG WKTQYARVSF STQRYSDVVK VVHRQGRILG YGFASAVISS ITIAGILAWK 490 500 510 IPGRLSPLPA LQSTVQLLGH VWTKISPKNT

References

[1]

"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H. Nature 438:1157-1161(2005) [PubMed: 16372010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP007151 Genomic DNA. Translation: BAE56474.1.
RefSeq	XP_001818476.1.
3D structure databases
SMR	Q2UPP1. Positions 316-427.
ModBase	Search...
Genome annotation databases
GeneID	5990421.
GenomeReviews	Gene locus bna4 in contig AP007151_GR.
KEGG	aor:AO090005001567.
Phylogenomic databases
OrthoDB	EOG9C2JSV.
PhylomeDB	Q2UPP1.
Family and domain databases
InterPro	IPR002938. mOase_FAD_bd. IPR003042. Rng_hydrolase-like. [Graphical view]
Pfam	PF01494. FAD_binding_3. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

KMO_ASPOR

Accession

Primary (citable) accession number: Q2UPP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	January 24, 2006
Last modified:	January 19, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)