Reviewed, UniProtKB/Swiss-Prot Q2UN31 (PMIP_ASPOR)

Names and origin

Protein names

Recommended name:
    Mitochondrial intermediate peptidase
      Short name=MIP
    EC=3.4.24.59
Alternative name(s):
    Octapeptidyl aminopeptidase

Gene names

Name:	oct1
ORF Names:	AO090001000525

Organism

Aspergillus oryzae [Complete proteome]

Taxonomic identifier

5062 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	800 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 23	23	Mitochondrion Potential
	Chain	24 – 800	777	Mitochondrial intermediate peptidase		PRO_0000338573
Sites
	Active site	564	1	By similarity
	Metal binding	563	1	Zinc; catalytic By similarity
	Metal binding	567	1	Zinc; catalytic By similarity
	Metal binding	570	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2UN31-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 474F93077541B66F	FASTA	800	89,243
	10 20 30 40 50 60 MAGHMLMPLR RRPWTCRACL QRLQQPRRSL ETAASPSSQS DVYDYAPTNH STQKKSNDET 70 80 90 100 110 120 LRRVFDSQPF WREFSQRSAT QSKPTGLVQN QYLTNPDGFR AFANVSLQRC QAIVAKVLAA 130 140 150 160 170 180 STLEEYRDMA RDLDRLSDLL CRVIDLSDFI RVIHPDPRVQ EAATQAYALM FEYMNVLNTT 190 200 210 220 230 240 TGLNDQLKKA ASNPDVTSYW SEEEKIVAQI LIKDFSNSAI HMPPNERQRF VNLSNDISQL 250 260 270 280 290 300 GSNFVNSAEP AKSQVVVGAN SLRGLDPILV QQIRRWNRTA SVPTTGMIPR LALRSVHDEG 310 320 330 340 350 360 VRREVYLATR TSSSRQLHRL EELLSKRAEL AQLSGHASFG HMTLSDKMAK SPEAVSNFLT 370 380 390 400 410 420 ALVGSNREYV QEELSKLQAM KGGSPLQPWD HAYYVHQRVL QYSQSRRSRE LSAVPEFFSL 430 440 450 460 470 480 GTVMQGLSRL FDRLYGVRLV PQETAAGETW NPDVRRLDVV DEAERHIAVI YCDLFSRPNK 490 500 510 520 530 540 HPNPAHFTLR CAREISSEEV AECATMDHSA HPNDGMATAV DPQSKTLRQL PTIALVCDFA 550 560 570 580 590 600 EPPATGAGRP SLLSEHSVRT LFHEMGHALH SILGQTRLQS ISGTRCATDF AELPSVLMER 610 620 630 640 650 660 FATEPAVLSM YARHWQTDQP LSESMMLSME KDRLAHGSIY GAVENEAQIL MALVDQAYHS 670 680 690 700 710 720 IPADKAGQID STAIYHQVSS AHSTLPDPTD SRPPTSWQGF FGHLYGYGAT YYSYIFDRAI 730 740 750 760 770 780 ANKIWEDVFQ AGKAAVDREA GERYKNEVLR WGGGRNGWDC VAGVLGSANA ANANGRLAEG 790 800 GDEAMREVGR WGLGRDGVSG

References

[1]

"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H. Nature 438:1157-1161(2005) [PubMed: 16372010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP007154 Genomic DNA. Translation: BAE57034.1.
RefSeq	XP_001819036.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M03.006.
Genome annotation databases
GeneID	5991007.
GenomeReviews	Gene locus oct1 in contig AP007154_GR.
KEGG	aor:AO090001000525.
Family and domain databases
InterPro	IPR001567. Pept_M3A_M3B. IPR006025. Pept_M_Zn_BS. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMIP_ASPOR

Accession

Primary (citable) accession number: Q2UN31

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	January 24, 2006
Last modified:	September 22, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)