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Protein

Mannosyl-oligosaccharide alpha-1,2-mannosidase 1B

Gene

mns1B

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.1 Publication

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.By similarity

Cofactori

Ca2+1 Publication, Mg2+1 PublicationNote: Ca2+. Can also use Mg2+, but with lower efficiency.1 Publication

pH dependencei

Optimum pH is 5.5. Activity is above 50 percent between pH 4.5 and 6.5.1 Publication

Temperature dependencei

Optimum temperature is 45 degrees Celsius. Activity remains at about 60 percent and 14 percent at 60 degrees Celsius and 65 degrees Celsius respectively.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei375Proton donorBy similarity1
Metal bindingi501CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandMetal-binding

Enzyme and pathway databases

UniPathwayiUPA00378

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47
mycoCLAPiMSD47A2_ASPOR

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide alpha-1,2-mannosidase 1B (EC:3.2.1.113By similarity)
Alternative name(s):
Class I alpha-mannosidase 1B
Man(9)-alpha-mannosidase 1B
Gene namesi
Name:mns1B
Synonyms:manA, msdS
ORF Names:AO090003000476
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006564 Componenti: Chromosome 2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000039482022 – 510Mannosyl-oligosaccharide alpha-1,2-mannosidase 1BAdd BLAST489

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi35N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi95N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi182N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi249N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi332 ↔ 361By similarity
Glycosylationi366N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi5062.CADAORAP00001087

Structurei

3D structure databases

ProteinModelPortaliQ2ULB2
SMRiQ2ULB2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000181987
KOiK01230
OMAiESFYYAY
OrthoDBiEOG092C1PJZ

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2ULB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHFSSLSLPL TALSLVTPSL AYPQFKFEQR VARSNSSESR ANAVKEAFVH
60 70 80 90 100
AWDGYMQYAY PHDELHPISN GVGDSRNGWG ASAVDALSTA VIMGNETIVN
110 120 130 140 150
QILDHIATID YSKTDDQVSL FETTIRYLGG MLSGYDLLKG PASNLVKDQA
160 170 180 190 200
KVKTLLDQSQ NLADVLKFAF DTPSGIPYNN INITSHGNDG ATTNGLAVTG
210 220 230 240 250
TLVLEWTRLS DLTGDTEYAQ LSQKAEDYLL NPSPKSAEPF EGLVGSHINI
260 270 280 290 300
SNGAFADGQV SWNGGDDSFY EYLIKMYVYD PKRFSTYGDR WVKAAESSIK
310 320 330 340 350
HLASHPEKRP DLTFLASYND GQYGLSSQHL TCFDGGSFLL GGTVLDRDDF
360 370 380 390 400
IQFGLDLVKG CHETYNQTLT GIGPESFGWD PKNVPSDQKE LYERAGFYIS
410 420 430 440 450
SGAYILRPEV IESFYYAWRI TGQEIYREWV WNAFVNINKY CRTDSGFAGL
460 470 480 490 500
TNVNAANGGG RYDNQESFLF AEVLKYVYLT FAPDNEWQVQ RGKGNKFVYN
510
TEAHPVRVAA
Length:510
Mass (Da):56,631
Last modified:January 24, 2006 - v1
Checksum:i5F24FEDB0B43F2BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB069647 Genomic DNA Translation: BAC07247.1
AP007155 Genomic DNA Translation: BAE57653.1
RefSeqiXP_001819655.1, XM_001819603.1

Genome annotation databases

EnsemblFungiiBAE57653; BAE57653; AO090003000476
GeneIDi5991638
KEGGiaor:AO090003000476

Similar proteinsi

Entry informationi

Entry nameiMNS1B_ASPOR
AccessioniPrimary (citable) accession number: Q2ULB2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: January 24, 2006
Last modified: March 28, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health