Q2UIM2 (ABFB_ASPOR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-N-arabinofuranosidase B
Short name=ABF B
Short name=Arabinosidase B
EC=3.2.1.55

Gene names

Name:	abfB
ORF Names:	AO090023000001

Organism

Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) [Complete proteome]

Taxonomic identifier

510516 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Alpha-N-arabinofuranosidase involved in the degradation of arabinoxylan, a major component of plant hemicellulose. Able to hydrolyze 1,5-, 1,3- and 1,2-alpha-linkages not only in L-arabinofuranosyl oligosaccharides, but also in polysaccharides containing terminal non-reducing L-arabinofuranoses in side chains, like L-arabinan, arabinogalactan and arabinoxylan. Ref.1
Catalytic activity	Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.
Pathway	Glycan metabolism; L-arabinan degradation.
Subcellular location	Secreted By similarity Ref.1.
Induction	Repressed by glucose. Ref.1
Domain	Organized into two domains: an N-terminal catalytic domain and a C-terminal arabinose-binding domain (ABD) By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 54 family.
Biophysicochemical properties	pH dependence: Optimum pH is 5.5. Stable between pH 4.0 and 6.5. Ref.1 Temperature dependence: Optimum temperature is 60 degrees Celsius.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation Xylan degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-arabinose metabolic process Inferred from electronic annotation. Source: InterPro arabinan metabolic process Inferred from electronic annotation. Source: InterPro polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	alpha-N-arabinofuranosidase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 506	480	Alpha-N-arabinofuranosidase B		PRO_0000394607
Regions
	Region	27 – 343	317	Catalytic By similarity
	Region	344 – 506	163	ABD By similarity
Sites
	Active site	229	1	Nucleophile By similarity
	Active site	305	1	Proton donor By similarity
	Binding site	227	1	Substrate By similarity
	Binding site	230	1	Substrate; via amide nitrogen By similarity
	Binding site	304	1	Substrate; via amide nitrogen By similarity
	Binding site	424	1	Substrate By similarity
	Binding site	426	1	Substrate; via amide nitrogen By similarity
	Binding site	427	1	Substrate; via amide nitrogen By similarity
	Binding site	443	1	Substrate By similarity
	Binding site	471	1	Substrate By similarity
	Binding site	473	1	Substrate; via amide nitrogen By similarity
	Binding site	476	1	Substrate; via amide nitrogen By similarity
	Binding site	496	1	Substrate By similarity
Amino acid modifications
	Glycosylation	91	1	N-linked (GlcNAc...) Potential
	Disulfide bond	29 ↔ 39		By similarity
	Disulfide bond	89 ↔ 94		By similarity
	Disulfide bond	184 ↔ 185		Uncommon non-proline cis-peptide bond By similarity
	Disulfide bond	409 ↔ 447		By similarity
Experimental info
	Sequence conflict	46	1	T → A in BAB71804. Ref.1
	Sequence conflict	84	1	I → T in BAB71803. Ref.1
	Sequence conflict	167	1	K → E in BAB71803. Ref.1
	Sequence conflict	387	1	S → T in BAB71803. Ref.1
	Sequence conflict	390	1	T → K in BAB71803. Ref.1
	Sequence conflict	393	1	R → K in BAB71803. Ref.1
	Sequence conflict	410	1	L → F in BAB71803. Ref.1
	Sequence conflict	429	1	L → H in BAB71803. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q2UIM2 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: A0D6B300C19C9420	FASTA	506	52,195
	10 20 30 40 50 60 MSSGLSLERA CAVALGIVAS ASLVAAGPCD IYSSGGTPCV AAHSTTRALY SAYTGALYQV 70 80 90 100 110 120 KRGSDGSTTD IAPLSAGGVA DAAIQDSFCA NTTCLITIIY DQSGRGNHLT QAPPGGFNGP 130 140 150 160 170 180 ESNGYDNLAS AVGAPVTLNG KKAYGVFMSP GTGYRNNAAS GTATGDKAEG MYAVLDGTHY 190 200 210 220 230 240 NSACCFDYGN AEVSNTDTGN GHMEAIYYGD NTVWGSGAGS GPWIMADLEN GLFSGLSSTN 250 260 270 280 290 300 NAGDPSISYR FVTAVVKGEA NQWSIRGANA ASGSLSTYYS GARPSASGYN PMSKEGAIIL 310 320 330 340 350 360 GIGGDNSNGA QGTFYEGVMT SGYPSDATEN SVQADIVAAK YAIASLTSGP ALTVGSSISL 370 380 390 400 410 420 QVTTAGYTTR YLAHDGSTVN TQVVSSSSTT ALRQQASWTV RTGLANSACL SFESVDTPGS 430 440 450 460 470 480 YIRHYNFALL LNANDGTKQF YEDATFCPQA GLNGQGNSIR SWSYPTRYFR HYENVLYVAS 490 500 NGGVQTFDAT TSFNDDVSWV VSTGFA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Synergistic degradation of arabinoxylan with alpha-L-arabinofuranosidase, xylanase and beta-xylosidase from soy sauce koji mold, Aspergillus oryzae, in high salt condition." Hashimoto T., Nakata Y. J. Biosci. Bioeng. 95:164-169(2003) [PubMed: 16233386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, INDUCTION, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: ATCC 42149 / RIB 40 and HL15.
[2]	"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H. Nature 438:1157-1161(2005) [PubMed: 16372010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB073860 Genomic DNA. Translation: BAB71803.1. AB073861 Genomic DNA. Translation: BAB71804.1. AP007157 Genomic DNA. Translation: BAE58593.1.
RefSeq	XP_001820595.1. XM_001820543.1.
3D structure databases
ProteinModelPortal	Q2UIM2.
SMR	Q2UIM2. Positions 27-506.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAORAT00007157; CADAORAP00007019; CADAORAG00007157.
GeneID	5993498.
GenomeReviews	Gene locus abfB in contig AP007157_GR.
KEGG	aor:AOR_1_144.
Phylogenomic databases
OrthoDB	EOG4N33X5.
Family and domain databases
InterPro	IPR015289. A-L-arabinofuranosidase_B_cat. IPR007934. AbfB. IPR008985. ConA-like_lec_gl. IPR013320. ConA-like_subgrp. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF05270. AbfB. 1 hit. PF09206. ArabFuran-catal. 1 hit. [Graphical view]
SUPFAM	SSF110221. AbfB. 1 hit. SSF49899. ConA_like_lec_gl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ABFB_ASPOR

Accession

Primary (citable) accession number: Q2UIM2
Secondary accession number(s): Q96VA0, Q96VA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program