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Protein

NADPH--cytochrome P450 reductase

Gene

cprA

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis (By similarity).By similarity

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.By similarity

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarity
  • FMNBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 765FMNPROSITE-ProRule annotationBy similarity
Nucleotide bindingi166 – 19732FMNPROSITE-ProRule annotationBy similarityAdd
BLAST
Nucleotide bindingi317 – 32812FADBy similarityAdd
BLAST
Nucleotide bindingi447 – 45812FADBy similarityAdd
BLAST
Nucleotide bindingi547 – 56519NADPBy similarityAdd
BLAST
Nucleotide bindingi642 – 65817NADPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseBy similarity (EC:1.6.2.4)
Short name:
CPRBy similarity
Short name:
P450RBy similarity
Gene namesi
Name:cprABy similarity
ORF Names:AO090023000520
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006564 Componenti: Chromosome 3

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity
  • Cell membrane By similarity; Single-pass membrane protein By similarity
  • Microsome By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 3123HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 695695NADPH--cytochrome P450 reductasePRO_0000404330Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5062.CADAORAP00007484.

Structurei

3D structure databases

ProteinModelPortaliQ2UHA7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 221156Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini277 – 538262FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Sequence Analysis
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0369.
HOGENOMiHOG000282027.
KOiK00327.
OMAiEIFPEND.
OrthoDBiEOG744TKC.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2UHA7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLDTLDLV VLVALLVGSV AYFTKGTYWA VAKDPYASSG PAMNGAAKAG
60 70 80 90 100
KTRDILEKME ETGKNCVIFY GSQTGTAEDY ASRLAKEGSQ RFGLKTMVAD
110 120 130 140 150
LEDYDYENLD KFPEDKVAFF VLATYGEGEP TDNAVEFYQF ITGEDVAFES
160 170 180 190 200
GASAEEKPLS ALKYVTFGLG NNTYEHYNAM VRNLDAALQK LGAQRIGSAG
210 220 230 240 250
EGDDGAGTME EDFLAWKEPM WTALSEAMGL QEREAVYEPV FNVTEDESKS
260 270 280 290 300
AEDETVYLGE PTKGHLEGQP KGPFSAHNPF IAPIVESREL FTVKDRNCLH
310 320 330 340 350
MEISIAGSNL TYQTGDHIAV WPTNAGAEVD RFLQVFGLEE KRHSVINIKG
360 370 380 390 400
IDVTAKVPIP TPTTYDAAVR YYMEVCAPVS RQFVSSLAAF APDEATKTEI
410 420 430 440 450
QRLGSDKDYF HEKITNQCFN IAQALQSITS KPFSAVPFSL LIEGLNKLQP
460 470 480 490 500
RYYSISSSSL VQKDKISITA VVESVRLPGA SHLVKGVTTN YLLALKQKQN
510 520 530 540 550
GEPSPDPHGL TYAITGPRNK YDGIHVPVHV RHSNFKLPSD PSRPIIMVGP
560 570 580 590 600
GTGVAPFRGF IQERAALAAK GEKVGTTVLF FGCRNRNEDF LYQDEFKAYE
610 620 630 640 650
EQLGDSLKII TAFSRETSQK VYVQHRLREQ AELVSDLLKQ KATFYVCGDA
660 670 680 690
ANMAREVNLV LGQIIAQQRG LPAEKGEEMV KHMRSSGSYQ EDVWS
Length:695
Mass (Da):76,637
Last modified:January 24, 2006 - v1
Checksum:i4C7A78DE7B4F2B63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007157 Genomic DNA. Translation: BAE59058.1.
RefSeqiXP_001821060.1. XM_001821008.2.

Genome annotation databases

EnsemblFungiiCADAORAT00007638; CADAORAP00007484; CADAORAG00007638.
GeneIDi5993062.
KEGGiaor:AOR_1_894144.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007157 Genomic DNA. Translation: BAE59058.1.
RefSeqiXP_001821060.1. XM_001821008.2.

3D structure databases

ProteinModelPortaliQ2UHA7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5062.CADAORAP00007484.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAORAT00007638; CADAORAP00007484; CADAORAG00007638.
GeneIDi5993062.
KEGGiaor:AOR_1_894144.

Phylogenomic databases

eggNOGiCOG0369.
HOGENOMiHOG000282027.
KOiK00327.
OMAiEIFPEND.
OrthoDBiEOG744TKC.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequencing and analysis of Aspergillus oryzae."
    Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
    , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
    Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 42149 / RIB 40.

Entry informationi

Entry nameiNCPR_ASPOR
AccessioniPrimary (citable) accession number: Q2UHA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: January 24, 2006
Last modified: April 1, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.