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Q2UHA7

- NCPR_ASPOR

UniProt

Q2UHA7 - NCPR_ASPOR

Protein

NADPH--cytochrome P450 reductase

Gene

cprA

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (24 Jan 2006)
      Previous versions | rss
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    Functioni

    This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis By similarity.By similarity

    Catalytic activityi

    NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.By similarity

    Cofactori

    FAD.By similarity
    FMN.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi72 – 765FMNBy similarityPROSITE-ProRule annotation
    Nucleotide bindingi166 – 19732FMNBy similarityPROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi317 – 32812FADBy similarityAdd
    BLAST
    Nucleotide bindingi447 – 45812FADBy similarityAdd
    BLAST
    Nucleotide bindingi547 – 56519NADPBy similarityAdd
    BLAST
    Nucleotide bindingi642 – 65817NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. FMN binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. NADPH-hemoprotein reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. sterol biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    FAD, Flavoprotein, FMN, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH--cytochrome P450 reductaseBy similarity (EC:1.6.2.4)
    Short name:
    CPRBy similarity
    Short name:
    P450RBy similarity
    Gene namesi
    Name:cprABy similarity
    ORF Names:AO090023000520
    OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
    Taxonomic identifieri510516 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000006564: Chromosome 3

    Subcellular locationi

    Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity. Cell membrane By similarity; Single-pass membrane protein By similarity. Microsome By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 695695NADPH--cytochrome P450 reductasePRO_0000404330Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi5062.CADAORAP00007484.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2UHA7.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 3123HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini66 – 221156Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini277 – 538262FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Sequence Analysis
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0369.
    HOGENOMiHOG000282027.
    KOiK00327.
    OMAiEFGAMEH.
    OrthoDBiEOG744TKC.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR023208. P450R.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000208. P450R. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2UHA7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQLDTLDLV VLVALLVGSV AYFTKGTYWA VAKDPYASSG PAMNGAAKAG    50
    KTRDILEKME ETGKNCVIFY GSQTGTAEDY ASRLAKEGSQ RFGLKTMVAD 100
    LEDYDYENLD KFPEDKVAFF VLATYGEGEP TDNAVEFYQF ITGEDVAFES 150
    GASAEEKPLS ALKYVTFGLG NNTYEHYNAM VRNLDAALQK LGAQRIGSAG 200
    EGDDGAGTME EDFLAWKEPM WTALSEAMGL QEREAVYEPV FNVTEDESKS 250
    AEDETVYLGE PTKGHLEGQP KGPFSAHNPF IAPIVESREL FTVKDRNCLH 300
    MEISIAGSNL TYQTGDHIAV WPTNAGAEVD RFLQVFGLEE KRHSVINIKG 350
    IDVTAKVPIP TPTTYDAAVR YYMEVCAPVS RQFVSSLAAF APDEATKTEI 400
    QRLGSDKDYF HEKITNQCFN IAQALQSITS KPFSAVPFSL LIEGLNKLQP 450
    RYYSISSSSL VQKDKISITA VVESVRLPGA SHLVKGVTTN YLLALKQKQN 500
    GEPSPDPHGL TYAITGPRNK YDGIHVPVHV RHSNFKLPSD PSRPIIMVGP 550
    GTGVAPFRGF IQERAALAAK GEKVGTTVLF FGCRNRNEDF LYQDEFKAYE 600
    EQLGDSLKII TAFSRETSQK VYVQHRLREQ AELVSDLLKQ KATFYVCGDA 650
    ANMAREVNLV LGQIIAQQRG LPAEKGEEMV KHMRSSGSYQ EDVWS 695
    Length:695
    Mass (Da):76,637
    Last modified:January 24, 2006 - v1
    Checksum:i4C7A78DE7B4F2B63
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP007157 Genomic DNA. Translation: BAE59058.1.
    RefSeqiXP_001821060.1. XM_001821008.2.

    Genome annotation databases

    EnsemblFungiiCADAORAT00007638; CADAORAP00007484; CADAORAG00007638.
    GeneIDi5993062.
    KEGGiaor:AOR_1_894144.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP007157 Genomic DNA. Translation: BAE59058.1 .
    RefSeqi XP_001821060.1. XM_001821008.2.

    3D structure databases

    ProteinModelPortali Q2UHA7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5062.CADAORAP00007484.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADAORAT00007638 ; CADAORAP00007484 ; CADAORAG00007638 .
    GeneIDi 5993062.
    KEGGi aor:AOR_1_894144.

    Phylogenomic databases

    eggNOGi COG0369.
    HOGENOMi HOG000282027.
    KOi K00327.
    OMAi EFGAMEH.
    OrthoDBi EOG744TKC.

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProi IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR023208. P450R.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000208. P450R. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequencing and analysis of Aspergillus oryzae."
      Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
      , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
      Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 42149 / RIB 40.

    Entry informationi

    Entry nameiNCPR_ASPOR
    AccessioniPrimary (citable) accession number: Q2UHA7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 8, 2011
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3