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Protein

NADPH--cytochrome P450 reductase

Gene

cprA

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.UniRule annotation

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei204 – 2041FMNUniRule annotation
Binding sitei296 – 2961NADPUniRule annotation
Binding sitei552 – 5521NADPUniRule annotation
Binding sitei656 – 6561NADPUniRule annotation
Binding sitei694 – 6941FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 776FMNUniRule annotation
Nucleotide bindingi123 – 1264FMNUniRule annotation
Nucleotide bindingi169 – 17810FMNUniRule annotation
Nucleotide bindingi451 – 4544FADUniRule annotation
Nucleotide bindingi469 – 4713FADUniRule annotation
Nucleotide bindingi486 – 4894FADUniRule annotation
Nucleotide bindingi614 – 6152NADPUniRule annotation
Nucleotide bindingi620 – 6245NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
Gene namesi
Name:cprAUniRule annotation
ORF Names:AO090023000520
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006564 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiFungiDB:AO090023000520.

Subcellular locationi

  • Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  • Mitochondrion outer membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  • Cell membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88LumenalUniRule annotation
Transmembranei9 – 3123HelicalUniRule annotationAdd
BLAST
Topological domaini32 – 695664CytoplasmicUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 695695NADPH--cytochrome P450 reductasePRO_0000404330Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ2UHA7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 221156Flavodoxin-likeUniRule annotationAdd
BLAST
Domaini277 – 538262FAD-binding FR-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000282027.
KOiK00327.
OMAiYTENCLE.
OrthoDBiEOG744TKC.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2UHA7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLDTLDLV VLVALLVGSV AYFTKGTYWA VAKDPYASSG PAMNGAAKAG
60 70 80 90 100
KTRDILEKME ETGKNCVIFY GSQTGTAEDY ASRLAKEGSQ RFGLKTMVAD
110 120 130 140 150
LEDYDYENLD KFPEDKVAFF VLATYGEGEP TDNAVEFYQF ITGEDVAFES
160 170 180 190 200
GASAEEKPLS ALKYVTFGLG NNTYEHYNAM VRNLDAALQK LGAQRIGSAG
210 220 230 240 250
EGDDGAGTME EDFLAWKEPM WTALSEAMGL QEREAVYEPV FNVTEDESKS
260 270 280 290 300
AEDETVYLGE PTKGHLEGQP KGPFSAHNPF IAPIVESREL FTVKDRNCLH
310 320 330 340 350
MEISIAGSNL TYQTGDHIAV WPTNAGAEVD RFLQVFGLEE KRHSVINIKG
360 370 380 390 400
IDVTAKVPIP TPTTYDAAVR YYMEVCAPVS RQFVSSLAAF APDEATKTEI
410 420 430 440 450
QRLGSDKDYF HEKITNQCFN IAQALQSITS KPFSAVPFSL LIEGLNKLQP
460 470 480 490 500
RYYSISSSSL VQKDKISITA VVESVRLPGA SHLVKGVTTN YLLALKQKQN
510 520 530 540 550
GEPSPDPHGL TYAITGPRNK YDGIHVPVHV RHSNFKLPSD PSRPIIMVGP
560 570 580 590 600
GTGVAPFRGF IQERAALAAK GEKVGTTVLF FGCRNRNEDF LYQDEFKAYE
610 620 630 640 650
EQLGDSLKII TAFSRETSQK VYVQHRLREQ AELVSDLLKQ KATFYVCGDA
660 670 680 690
ANMAREVNLV LGQIIAQQRG LPAEKGEEMV KHMRSSGSYQ EDVWS
Length:695
Mass (Da):76,637
Last modified:January 24, 2006 - v1
Checksum:i4C7A78DE7B4F2B63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007157 Genomic DNA. Translation: BAE59058.1.
RefSeqiXP_001821060.1. XM_001821008.2.

Genome annotation databases

EnsemblFungiiCADAORAT00007638; CADAORAP00007484; CADAORAG00007638.
GeneIDi5993062.
KEGGiaor:AOR_1_894144.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007157 Genomic DNA. Translation: BAE59058.1.
RefSeqiXP_001821060.1. XM_001821008.2.

3D structure databases

ProteinModelPortaliQ2UHA7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAORAT00007638; CADAORAP00007484; CADAORAG00007638.
GeneIDi5993062.
KEGGiaor:AOR_1_894144.

Organism-specific databases

EuPathDBiFungiDB:AO090023000520.

Phylogenomic databases

HOGENOMiHOG000282027.
KOiK00327.
OMAiYTENCLE.
OrthoDBiEOG744TKC.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequencing and analysis of Aspergillus oryzae."
    Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
    , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
    Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 42149 / RIB 40.

Entry informationi

Entry nameiNCPR_ASPOR
AccessioniPrimary (citable) accession number: Q2UHA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: January 24, 2006
Last modified: June 8, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.