Q2UFN0 (GUAA_ASPOR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing]
EC=6.3.5.2

Alternative name(s):
GMP synthetase
Glutamine amidotransferase

Gene names

Name:	gua1
ORF Names:	AO090026000141

Organism

Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) [Complete proteome]

Taxonomic identifier

510516 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 541	541	GMP synthase [glutamine-hydrolyzing]		PRO_0000286145
Regions
	Domain	15 – 209	195	Glutamine amidotransferase type-1
	Domain	210 – 416	207	GMPS ATP-PPase
	Nucleotide binding	238 – 244	7	ATP By similarity
Sites
	Active site	91	1	For GATase activity By similarity
	Active site	183	1	For GATase activity By similarity
	Active site	185	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2UFN0 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: EFB3F9AB66CC38AC	FASTA	541	59,794
	10 20 30 40 50 60 MAETPELEPH NAFDTILTLD FGSQYTHLIT RRLRELNVYS EMLPCTQKLA DLKFKPAGVI 70 80 90 100 110 120 LSGGPYSVYE EGAPHVDPAY FDLGVPILGI CYGLQEIAYR LDSTNVIAGT SREYGLAQLK 130 140 150 160 170 180 AKKVGGHVDH LFDGLEDEFN VWMSHGDKLG KLPEGFHTIA TTPNSEYAGI AHETKPIYGL 190 200 210 220 230 240 QLHPEVTHTQ NGTKLLKNFA VNICGCKQNW TMARFVDQEI ARIRKLVGPT GQVLGAVSGG 250 260 270 280 290 300 VDSTVAAKLM KEAIGDRFHA VLVDTGFMRL NECEQVKQTL AEHLGINLIV ADASQVFMEG 310 320 330 340 350 360 LKGISDPEQK RKFIGNKFID VFEEEAKKIE DAAAHSETAG KIGFFLQGTL YPDVIESISF 370 380 390 400 410 420 KGPSATIKTH HNVGGLPKRM TEGQGLKLIE PLRELFKDEV RDLGRQLGIA HEMVMRHPFP 430 440 450 460 470 480 GPGIAIRILG EITPERVEMA RKADHIFISM IREAGLYDKI GQAFAALDPS RAVGVMGDKR 490 500 510 520 530 540 VYENIVLLRA VETTDFMTAI AYPFEHEFLT RVSTRIVNEV SGVCRVAYDY TSKPPGTIEL E

References

[1]

"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H. Nature 438:1157-1161(2005) [PubMed: 16372010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP007159 Genomic DNA. Translation: BAE59635.1.
RefSeq	XP_001821637.1. XM_001821585.2.
3D structure databases
ProteinModelPortal	Q2UFN0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2UFN0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAORAT00008219; CADAORAP00008053; CADAORAG00008219.
GeneID	5993665.
GenomeReviews	Gene locus gua1 in contig AP007159_GR.
KEGG	aor:AOR_1_270014.
Phylogenomic databases
GeneTree	EFGT00050000004027.
OrthoDB	EOG4WWVST.
Family and domain databases
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_ASPOR

Accession

Primary (citable) accession number: Q2UFN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program