Q2U9M7 (BGLH_ASPOR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-glucosidase H
EC=3.2.1.21

Alternative name(s):
Beta-D-glucoside glucohydrolase H
Cellobiase H
Gentiobiase H

Gene names

Name:	bglH
ORF Names:	AO090166000090

Organism

Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) [Complete proteome]

Taxonomic identifier

510516 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus oryzae

Protein attributes

Sequence length	827 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Beta-glucosidases are one of a number of cellulolytic enzymes involved in the degradation of cellulosic biomass. Catalyzes the last step releasing glucose from the inhibitory cellobiose By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Pathway	Glycan metabolism; cellulose degradation.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family. Contains 1 PA14 domain.
Sequence caution	The sequence BAE61738.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 827	827	Probable beta-glucosidase H		PRO_0000394880
Regions
	Domain	424 – 519	96	PA14
Sites
	Active site	223	1	By similarity
Amino acid modifications
	Glycosylation	471	1	N-linked (GlcNAc...) Potential
	Glycosylation	594	1	N-linked (GlcNAc...) Potential
	Glycosylation	600	1	N-linked (GlcNAc...) Potential
	Glycosylation	625	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2U9M7 [UniParc]. Last modified June 15, 2010. Version 2. Checksum: 7D495C5BD7FBF59C	FASTA	827	90,680
	10 20 30 40 50 60 MALEIDYVLS HISQEDKIAL LAGIDFWHTH PIPELNVPSI RSTDGPNGIR GTKFFAGVPA 70 80 90 100 110 120 ACLPCGTALA STWDQNLLRE VGVLIGKECL AKGAHCWLGP TINMPRSPLG GRGFESFAED 130 140 150 160 170 180 PHLAGAMAAS MITGCESTGV ISAVKHFVGN DQEHERRAVD VLVTQRALRE IYLRPFQIVA 190 200 210 220 230 240 RDAGPGALMT SYNKINGKHV VESKEMLDMV RQEWKWNPLI MSDWLGTYTT IDSMNAGLDL 250 260 270 280 290 300 EMPGPSRYRG RYVESALQAR LIKESTIDSR ARKVLEFVQQ ASRAPVSAVE TGRDYPEDRA 310 320 330 340 350 360 LNRNLCANSI VLLKNQNDIL PLPKTIKKIA LVGSHVRTPA ISGGGSASLE PYYTVSLYDA 370 380 390 400 410 420 VSEALPHTEI LYEVGAYAHK MLPVIDRLLT NAVMHFYNEP VGTERILRAT QPMSKTAFQL 430 440 450 460 470 480 MDFNAPELNR GLFYATLTGD FTPDVSGVWD FGLTVFGTGL LYVDDELVVD NTTHQTRGTA 490 500 510 520 530 540 FFGKGTVQEL GSKTLNAGQT YKIRIEYGSA NTSPMKAIGV VHFGGGAAHL GACLHVDSAE 550 560 570 580 590 600 MVRSAVKAAA EADYTILCTG LNHEWESEGF DRSHMDLPPG IDALITSVLD VAANKTVIVN 610 620 630 640 650 660 QSGTPVTMPW ADRARGIVQA WYGGNETGHG IADVIFGDVN PSGKLPLSWP VDVKHNPAYL 670 680 690 700 710 720 NYASVGGRVL YGEDVYVGYR YYEKVGREVL FPFGHGLSYT TFTVSPDVVF SQEVFRPEEP 730 740 750 760 770 780 PTAAVKIKNT GKVAGAQVLQ LYISAPHSPT PRPTKELHGF TKVLLQPGEE RVAHIRMDKY 790 800 810 820 ATNFWDEIEG MWKSEEGIYE ALIGTSSQNI LAKGTFRVDR TRYWLGL

References

[1]

"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H. Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP007163 Genomic DNA. Translation: BAE61738.1. Sequence problems.
RefSeq	XP_001822871.2. XM_001822819.2.
3D structure databases
ProteinModelPortal	Q2U9M7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAORAT00012146; CADAORAP00011903; CADAORAG00012146.
GeneID	5994927.
KEGG	aor:AOR_1_130124.
Phylogenomic databases
eggNOG	COG1472.
HOGENOM	HOG000031215.
OrthoDB	EOG4RFQ1P.
Enzyme and pathway databases
UniPathway	UPA00696.
Family and domain databases
Gene3D	3.20.20.300. 1 hit. 3.40.50.1700. 2 hits.
InterPro	IPR026891. Fn3-like. IPR026892. Glyco_hydro_3. IPR002772. Glyco_hydro_3_C. IPR001764. Glyco_hydro_3_N. IPR017853. Glycoside_hydrolase_SF. IPR011658. PA14. [Graphical view]
PANTHER	PTHR30620. PTHR30620. 1 hit.
Pfam	PF14310. Fn3-like. 1 hit. PF00933. Glyco_hydro_3. 1 hit. PF01915. Glyco_hydro_3_C. 1 hit. PF07691. PA14. 1 hit. [Graphical view]
PRINTS	PR00133. GLHYDRLASE3.
SMART	SM00758. PA14. 1 hit. [Graphical view]
SUPFAM	SSF52279. Glyco_hydro_3_C. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGLH_ASPOR

Accession

Primary (citable) accession number: Q2U9M7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	June 15, 2010
Last modified:	May 1, 2013
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program