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Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei90SubstrateBy similarity1
Binding sitei135SubstrateBy similarity1
Binding sitei136Substrate; via amide nitrogenBy similarity1
Binding sitei137SubstrateBy similarity1
Binding sitei195SubstrateBy similarity1
Active sitei196Proton donorSequence analysis1
Binding sitei264SubstrateBy similarity1
Active sitei309NucleophileSequence analysis1
Binding sitei374SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase B (EC:3.2.1.23)
Alternative name(s):
Lactase B
Gene namesi
Name:lacB
ORF Names:AO090120000158
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006564 Componenti: Chromosome 5

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000039522623 – 1022Probable beta-galactosidase BAdd BLAST1000

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25N-linked (GlcNAc...)Sequence analysis1
Glycosylationi111N-linked (GlcNAc...)Sequence analysis1
Glycosylationi172N-linked (GlcNAc...)Sequence analysis1
Glycosylationi210N-linked (GlcNAc...)Sequence analysis1
Glycosylationi251N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi270 ↔ 325By similarity
Glycosylationi271N-linked (GlcNAc...)Sequence analysis1
Glycosylationi412N-linked (GlcNAc...)Sequence analysis1
Glycosylationi457N-linked (GlcNAc...)Sequence analysis1
Glycosylationi551N-linked (GlcNAc...)Sequence analysis1
Glycosylationi598N-linked (GlcNAc...)Sequence analysis1
Glycosylationi627N-linked (GlcNAc...)Sequence analysis1
Glycosylationi704N-linked (GlcNAc...)Sequence analysis1
Glycosylationi749N-linked (GlcNAc...)Sequence analysis1
Glycosylationi787N-linked (GlcNAc...)Sequence analysis1
Glycosylationi821N-linked (GlcNAc...)Sequence analysis1
Glycosylationi882N-linked (GlcNAc...)Sequence analysis1
Glycosylationi921N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ2U6P1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000181922.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2U6P1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLISKTVLSG LALGASFVGV SAQQNSTRWP LHDNGLTDTV EWDHYSFLIN
60 70 80 90 100
GQRHFVFSGE FHYWRIPVPE LWRDLLEKIK AAGFTAFSIY NHWGYHSPKP
110 120 130 140 150
GVLDFENGAH NFTSIMTLAK EIGLYMIIRP GPYVNAEANA GGLPLWTTTG
160 170 180 190 200
AYGKLRDNDP RYLEALTPYW ANISKIIAPH LITNDGNVIL YQIENEYAEQ
210 220 230 240 250
WLDEETHEPN TSGQEYMQYL EDVARENGID APLIHNLPNM NGHSWSKDLS
260 270 280 290 300
NATGNVDVIG VDSYPTCWTC NVSECASTNG EYIPYLKLTY PQISYFKELS
310 320 330 340 350
PTQPSFMPEF QGGSYNPWGG PQGGCPDDLG PDFANLFYRN LISQRVSAIS
360 370 380 390 400
LYMLYGGTNW GWHASTDVAT SYDYSSPISE NRKLIEKYYE TKVLTQFTKI
410 420 430 440 450
AQDLSKVDRL GNSTKYSSNP AVSVAELRNP DTGAAFYVTQ HEYTPSGTVE
460 470 480 490 500
KFTVKVNTSE GALTIPQYGS QITLNGHQSK IIVTDFKFGS KTLLYSTAEV
510 520 530 540 550
LTYAVIDGKE VLALWVPTGE SGEFTVKGVN SAKFADKGRT ANIEIHPGTN
560 570 580 590 600
NVTVSFMQRS GMSLVELGDG TRIVLLDRSA AHVFWSTPLN NDPAEAGNNT
610 620 630 640 650
VLVHGPYLVR SAKLEGCDLK LTGDIQNSTE VSIFAPKSVC SVNWNGKKTS
660 670 680 690 700
VKSAKGGVIT TTLGGDAKFE LPTISGWKSA DSLPEIAKDY SATSKAWVVA
710 720 730 740 750
TKTNSSNPTP PAPNNPVLYV DENDIHVGNH IYRATFPSTD EPPTDVYLNI
760 770 780 790 800
TGGRAFGYSV WLNSDFIGSW LGTATTEQND QTFSFSNATL STDEDNILVV
810 820 830 840 850
VMDNSAHDLR DGALNPRGIT NATLIGPGSY SFTEWKLAGN AGFEDHLDPV
860 870 880 890 900
RAPLNEGSLY AERVGIHLPG YEFDEAEEVS SNSTSLTVPG AGIRVFRTVV
910 920 930 940 950
PLSVPQGLDV SISFRLTAPS NVTFTSAEGY TNQLRALLFV NGYQYGRFNP
960 970 980 990 1000
YIGHQIDFPV PPGVLDYNGD NTIAVTVWSQ SVDGAEIKVD WNVDYVHETS
1010 1020
FDMNFDGAYL RPGWIEERRE YA
Length:1,022
Mass (Da):112,493
Last modified:July 13, 2010 - v2
Checksum:iE18455942DAA8A62
GO

Sequence cautioni

The sequence BAE62774 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007166 Genomic DNA. Translation: BAE62774.1. Sequence problems.

Genome annotation databases

EnsemblFungiiBAE62774; BAE62774; AO090120000158.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007166 Genomic DNA. Translation: BAE62774.1. Sequence problems.

3D structure databases

ProteinModelPortaliQ2U6P1.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAE62774; BAE62774; AO090120000158.

Phylogenomic databases

HOGENOMiHOG000181922.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBGALB_ASPOR
AccessioniPrimary (citable) accession number: Q2U6P1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 13, 2010
Last modified: November 2, 2016
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.