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Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei90SubstrateBy similarity1
Binding sitei135SubstrateBy similarity1
Binding sitei136Substrate; via amide nitrogenBy similarity1
Binding sitei137SubstrateBy similarity1
Binding sitei195SubstrateBy similarity1
Active sitei196Proton donorSequence analysis1
Binding sitei264SubstrateBy similarity1
Active sitei309NucleophileSequence analysis1
Binding sitei374SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH35 Glycoside Hydrolase Family 35

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase B (EC:3.2.1.23)
Alternative name(s):
Lactase B
Gene namesi
Name:lacB
ORF Names:AO090120000158
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006564 Componenti: Chromosome 5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei1261 Asp o Lactase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000039522623 – 1022Probable beta-galactosidase BAdd BLAST1000

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi111N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi172N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi210N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi251N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi270 ↔ 325By similarity
Glycosylationi271N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi412N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi457N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi551N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi598N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi627N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi704N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi749N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi787N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi821N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi882N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi921N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ2U6P1

Structurei

3D structure databases

ProteinModelPortaliQ2U6P1
SMRiQ2U6P1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000181922
OrthoDBiEOG092C0SLI

Family and domain databases

Gene3Di2.102.20.10, 1 hit
2.60.120.260, 3 hits
2.60.390.10, 1 hit
InterProiView protein in InterPro
IPR018954 Betagal_dom2
IPR037110 Betagal_dom2_sf
IPR025972 BetaGal_dom3
IPR036833 BetaGal_dom3_sf
IPR025300 BetaGal_jelly_roll_dom
IPR008979 Galactose-bd-like_sf
IPR031330 Gly_Hdrlase_35_cat
IPR001944 Glycoside_Hdrlase_35
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR23421 PTHR23421, 1 hit
PfamiView protein in Pfam
PF10435 BetaGal_dom2, 1 hit
PF13363 BetaGal_dom3, 1 hit
PF13364 BetaGal_dom4_5, 2 hits
PF01301 Glyco_hydro_35, 1 hit
PRINTSiPR00742 GLHYDRLASE35
SMARTiView protein in SMART
SM01029 BetaGal_dom2, 1 hit
SUPFAMiSSF117100 SSF117100, 1 hit
SSF49785 SSF49785, 2 hits
SSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2U6P1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLISKTVLSG LALGASFVGV SAQQNSTRWP LHDNGLTDTV EWDHYSFLIN
60 70 80 90 100
GQRHFVFSGE FHYWRIPVPE LWRDLLEKIK AAGFTAFSIY NHWGYHSPKP
110 120 130 140 150
GVLDFENGAH NFTSIMTLAK EIGLYMIIRP GPYVNAEANA GGLPLWTTTG
160 170 180 190 200
AYGKLRDNDP RYLEALTPYW ANISKIIAPH LITNDGNVIL YQIENEYAEQ
210 220 230 240 250
WLDEETHEPN TSGQEYMQYL EDVARENGID APLIHNLPNM NGHSWSKDLS
260 270 280 290 300
NATGNVDVIG VDSYPTCWTC NVSECASTNG EYIPYLKLTY PQISYFKELS
310 320 330 340 350
PTQPSFMPEF QGGSYNPWGG PQGGCPDDLG PDFANLFYRN LISQRVSAIS
360 370 380 390 400
LYMLYGGTNW GWHASTDVAT SYDYSSPISE NRKLIEKYYE TKVLTQFTKI
410 420 430 440 450
AQDLSKVDRL GNSTKYSSNP AVSVAELRNP DTGAAFYVTQ HEYTPSGTVE
460 470 480 490 500
KFTVKVNTSE GALTIPQYGS QITLNGHQSK IIVTDFKFGS KTLLYSTAEV
510 520 530 540 550
LTYAVIDGKE VLALWVPTGE SGEFTVKGVN SAKFADKGRT ANIEIHPGTN
560 570 580 590 600
NVTVSFMQRS GMSLVELGDG TRIVLLDRSA AHVFWSTPLN NDPAEAGNNT
610 620 630 640 650
VLVHGPYLVR SAKLEGCDLK LTGDIQNSTE VSIFAPKSVC SVNWNGKKTS
660 670 680 690 700
VKSAKGGVIT TTLGGDAKFE LPTISGWKSA DSLPEIAKDY SATSKAWVVA
710 720 730 740 750
TKTNSSNPTP PAPNNPVLYV DENDIHVGNH IYRATFPSTD EPPTDVYLNI
760 770 780 790 800
TGGRAFGYSV WLNSDFIGSW LGTATTEQND QTFSFSNATL STDEDNILVV
810 820 830 840 850
VMDNSAHDLR DGALNPRGIT NATLIGPGSY SFTEWKLAGN AGFEDHLDPV
860 870 880 890 900
RAPLNEGSLY AERVGIHLPG YEFDEAEEVS SNSTSLTVPG AGIRVFRTVV
910 920 930 940 950
PLSVPQGLDV SISFRLTAPS NVTFTSAEGY TNQLRALLFV NGYQYGRFNP
960 970 980 990 1000
YIGHQIDFPV PPGVLDYNGD NTIAVTVWSQ SVDGAEIKVD WNVDYVHETS
1010 1020
FDMNFDGAYL RPGWIEERRE YA
Length:1,022
Mass (Da):112,493
Last modified:July 13, 2010 - v2
Checksum:iE18455942DAA8A62
GO

Sequence cautioni

The sequence BAE62774 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007166 Genomic DNA Translation: BAE62774.1 Sequence problems.

Genome annotation databases

EnsemblFungiiBAE62774; BAE62774; AO090120000158

Similar proteinsi

Entry informationi

Entry nameiBGALB_ASPOR
AccessioniPrimary (citable) accession number: Q2U6P1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 13, 2010
Last modified: May 23, 2018
This is version 72 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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