Reviewed, UniProtKB/Swiss-Prot Q2U6A1 (DNLI4_ASPOR)

Names and origin

Protein names

Recommended name:
    DNA ligase 4
    EC=6.5.1.1
Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	lig4
ORF Names:	AO090120000322

Organism

Aspergillus oryzae [Complete proteome]

Taxonomic identifier

5062 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	1006 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Magnesium By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1006	1006	DNA ligase 4		PRO_0000278376
Regions
	Domain	718 – 811	94	BRCT 1
	Domain	890 – 1002	113	BRCT 2
Sites
	Active site	320	1	N6-AMP-lysine intermediate By similarity
	Metal binding	387	1	Magnesium 1 Potential
	Metal binding	487	1	Magnesium 2 Potential
	Binding site	318	1	ATP By similarity
	Binding site	325	1	ATP By similarity
	Binding site	347	1	ATP By similarity
	Binding site	492	1	ATP By similarity
	Binding site	503	1	ATP By similarity
	Binding site	509	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2U6A1-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 75D212090D2D03E4	FASTA	1,006	114,882
	10 20 30 40 50 60 MDSDDDYNGP ADTNPRLEDE ESDLDEKYPN RPRNHSTTLP FHVLFQTLFY PLSEIKKKPA 70 80 90 100 110 120 GPARKKVGPH GLSSVNLTPL EKRRDIIDRF ISRWRKEVGD DIYPAFRLIL PDKDRDRAMY 130 140 150 160 170 180 GMKEKAIGKL LIRIMKIDKN SEDALNLLNW KLPGQTTTSS MAGDFAGRCF GVLSKRPMRT 190 200 210 220 230 240 EVGDMTIEEV NEKLDHLSAA SKENQQLPIL TEFYRRMNPE ELMWLIRIIL RQMKVGATER 250 260 270 280 290 300 TFFDVFHPDA ENLYSISSSL RRVCWELHDP NIRLEAEDRG ITLMQCFQPQ LAQFQMHSLD 310 320 330 340 350 360 RMISRMRLTE DDPVFWIEEK LDGERMQLHM DSNDSVPGGR TFRFWSRKAK DYTYLYGNGI 370 380 390 400 410 420 QDENGALTRY LSDAFADGVE SLILDGEMIT WDTEQDAIAP FGTLKTAALS EQRNPYSSTT 430 440 450 460 470 480 RPLFRIFDIL YLNGRDLTRY TLRDRRNALQ KSIKPVYRRF EIHPYEEATG KTEIEEALRR 490 500 510 520 530 540 VVEEASEGLV LKNPRSPYRL NERHDDWMKV KPEYMTEFGE SLDVVVIGGY YGSGHRGGGL 550 560 570 580 590 600 SSFLCGLRVD DAHSSQGMVA SKCYSFCKVG GGFTAADYAN IRHHTDGKWH EWKSRKPPTT 610 620 630 640 650 660 YIELAGGDAQ YERPDMWIKP EDSVVLCVKA ASVAVSDQFR IGLTLRFPRF KKLRMDKDWK 670 680 690 700 710 720 SALSVQEFLD LKANAERERK EKEFNVDNSR KKRAKRDNKK PLAIVGYSAE AEAQYTGPSG 730 740 750 760 770 780 NIFEGLNFYI TTDSNTPVKK SKAELEQLVK ANGGKFFQTS NAAPSTICIA DRRTVKAASL 790 800 810 820 830 840 QKSGNVNIIR PSWILDCIRQ SEIDAGLPDS LLPLEPRHVF FATQDKKEEI AASVDRFNDS 850 860 870 880 890 900 YARNTTNDEL KEILKQMSKD HHFHASQNPK IVRKLNERIQ EKVNAGWEMP SGWLFKGLTI 910 920 930 940 950 960 LFPQNDKVDD AEVDETSQTH QQYRLNLARN TVRFAGANVV DSKSSSVTHI VVAPGSSSSD 970 980 990 1000 VSSIRKSHSA KPGKKVPHLV TAEWIEECWK QRTLLGEEGF QPSRGT

References

[1]

"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H. Nature 438:1157-1161(2005) [PubMed: 16372010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP007166 Genomic DNA. Translation: BAE62914.1.
RefSeq	XP_001824047.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5996306.
KEGG	aor:AO090120000322.
Enzyme and pathway databases
BRENDA	6.5.1.1. 2240.
Family and domain databases
InterPro	IPR001357. BRCT. IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 2 hits. PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS50172. BRCT. 2 hits. PS00697. DNA_LIGASE_A1. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_ASPOR

Accession

Primary (citable) accession number: Q2U6A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	January 24, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)