DNA ligase 4 - Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)

Preferred order of sections

Names and origin

Protein names

Recommended name:
DNA ligase 4
EC=6.5.1.1

Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	lig4
ORF Names:	AO090120000322

Organism

Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) [Complete proteome]

Taxonomic identifier

510516 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	1006 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Magnesium By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1006

1006

DNA ligase 4

PRO_0000278376

Regions

Domain

718 – 811

94

BRCT 1

Domain

890 – 1002

113

BRCT 2

Sites

Active site

320

1

N6-AMP-lysine intermediate By similarity

Metal binding

387

1

Magnesium 1 Potential

Metal binding

487

1

Magnesium 2 Potential

Binding site

318

1

ATP By similarity

Binding site

325

1

ATP By similarity

Binding site

347

1

ATP By similarity

Binding site

492

1

ATP By similarity

Binding site

503

1

ATP By similarity

Binding site

509

1

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q2U6A1 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: 75D212090D2D03E4
Show »

FASTA

1,006

114,882

        10         20         30         40         50         60 
MDSDDDYNGP ADTNPRLEDE ESDLDEKYPN RPRNHSTTLP FHVLFQTLFY PLSEIKKKPA 

        70         80         90        100        110        120 
GPARKKVGPH GLSSVNLTPL EKRRDIIDRF ISRWRKEVGD DIYPAFRLIL PDKDRDRAMY 

       130        140        150        160        170        180 
GMKEKAIGKL LIRIMKIDKN SEDALNLLNW KLPGQTTTSS MAGDFAGRCF GVLSKRPMRT 

       190        200        210        220        230        240 
EVGDMTIEEV NEKLDHLSAA SKENQQLPIL TEFYRRMNPE ELMWLIRIIL RQMKVGATER 

       250        260        270        280        290        300 
TFFDVFHPDA ENLYSISSSL RRVCWELHDP NIRLEAEDRG ITLMQCFQPQ LAQFQMHSLD 

       310        320        330        340        350        360 
RMISRMRLTE DDPVFWIEEK LDGERMQLHM DSNDSVPGGR TFRFWSRKAK DYTYLYGNGI 

       370        380        390        400        410        420 
QDENGALTRY LSDAFADGVE SLILDGEMIT WDTEQDAIAP FGTLKTAALS EQRNPYSSTT 

       430        440        450        460        470        480 
RPLFRIFDIL YLNGRDLTRY TLRDRRNALQ KSIKPVYRRF EIHPYEEATG KTEIEEALRR 

       490        500        510        520        530        540 
VVEEASEGLV LKNPRSPYRL NERHDDWMKV KPEYMTEFGE SLDVVVIGGY YGSGHRGGGL 

       550        560        570        580        590        600 
SSFLCGLRVD DAHSSQGMVA SKCYSFCKVG GGFTAADYAN IRHHTDGKWH EWKSRKPPTT 

       610        620        630        640        650        660 
YIELAGGDAQ YERPDMWIKP EDSVVLCVKA ASVAVSDQFR IGLTLRFPRF KKLRMDKDWK 

       670        680        690        700        710        720 
SALSVQEFLD LKANAERERK EKEFNVDNSR KKRAKRDNKK PLAIVGYSAE AEAQYTGPSG 

       730        740        750        760        770        780 
NIFEGLNFYI TTDSNTPVKK SKAELEQLVK ANGGKFFQTS NAAPSTICIA DRRTVKAASL 

       790        800        810        820        830        840 
QKSGNVNIIR PSWILDCIRQ SEIDAGLPDS LLPLEPRHVF FATQDKKEEI AASVDRFNDS 

       850        860        870        880        890        900 
YARNTTNDEL KEILKQMSKD HHFHASQNPK IVRKLNERIQ EKVNAGWEMP SGWLFKGLTI 

       910        920        930        940        950        960 
LFPQNDKVDD AEVDETSQTH QQYRLNLARN TVRFAGANVV DSKSSSVTHI VVAPGSSSSD 

       970        980        990       1000 
VSSIRKSHSA KPGKKVPHLV TAEWIEECWK QRTLLGEEGF QPSRGT

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References

[1]

"Genome sequencing and analysis of Aspergillus oryzae."
Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.

Kikuchi H.
Nature 438:1157-1161(2005) [PubMed: 16372010] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP007166 Genomic DNA. Translation: BAE62914.1.
3D structure databases
ProteinModelPortal	Q2U6A1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2U6A1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAORAT00011462; CADAORAP00011229; CADAORAG00011462.
GenomeReviews	Gene locus lig4 in contig AP007166_GR.
Phylogenomic databases
GeneTree	EFGT00050000002597.
OrthoDB	EOG41K2MD.
Family and domain databases
InterPro	IPR001357. BRCT. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 2 hits. [Graphical view]
SUPFAM	SSF52113. BRCT. 2 hits. SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00574. Dnl1. 1 hit.
PROSITE	PS50172. BRCT. 2 hits. PS00697. DNA_LIGASE_A1. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_ASPOR

Accession

Primary (citable) accession number: Q2U6A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents