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Protein

Bifunctional lycopene cyclase/phytoene synthase

Gene

AO090020000159

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and lycopene to beta-carotene via the intermediate gamma-carotene (lycopene cyclase).By similarity

Catalytic activityi

Carotenoid psi-end group = carotenoid beta-end group.
2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Pathwayi

GO - Molecular functioni

  1. geranylgeranyl-diphosphate geranylgeranyltransferase activity Source: UniProtKB-EC
  2. intramolecular lyase activity Source: InterPro

GO - Biological processi

  1. carotenoid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Transferase

Keywords - Biological processi

Carotenoid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00799; UER00773.
UPA00802.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional lycopene cyclase/phytoene synthase
Including the following 2 domains:
Lycopene beta-cyclase (EC:5.5.1.19)
Alternative name(s):
Lycopene cyclase
Phytoene synthase (EC:2.5.1.32)
Gene namesi
ORF Names:AO090020000159
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006564 Componenti: Chromosome 6

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei5 – 2521HelicalSequence AnalysisAdd
BLAST
Transmembranei35 – 5521HelicalSequence AnalysisAdd
BLAST
Transmembranei65 – 8521HelicalSequence AnalysisAdd
BLAST
Transmembranei91 – 11121HelicalSequence AnalysisAdd
BLAST
Transmembranei116 – 13621HelicalSequence AnalysisAdd
BLAST
Transmembranei145 – 16521HelicalSequence AnalysisAdd
BLAST
Transmembranei218 – 23821HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 587587Bifunctional lycopene cyclase/phytoene synthasePRO_0000409233Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5062.CADAORAP00006543.

Structurei

3D structure databases

ProteinModelPortaliQ2U4X9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 236236Lycopene beta-cyclaseAdd
BLAST
Regioni243 – 587345Phytoene synthaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the lycopene beta-cyclase family.Curated
In the C-terminal section; belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG47964.
KOiK17841.
OMAiEVFFFII.
OrthoDBiEOG7RBZJ2.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR03462. CarR_dom_SF. 2 hits.

Sequencei

Sequence statusi: Complete.

Q2U4X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLDYILVHV TYNIPLAGIL TLVYWPFMTR LDWQKISTLV IISLVATIPW
60 70 80 90 100
DSYLVRHRIW TYAPNGVIGW TLYDIPSEEV FFFIIQTYNT SLVYLILTRW
110 120 130 140 150
LVLPMYLGTV ARKETLIGAS ILLLAISVGL IALCFGDHFT YFGMIITWAG
160 170 180 190 200
PFLLIQWVFS SGFIIALPKL ELMVSITLPT LFLWTVDTIS INQGTWTVEA
210 220 230 240 250
PTKLGVQLWS GMDIEEVLFF LITNIVIVFG LVCIDYAIAM ATCELVQSPQ
260 270 280 290 300
AVQSFPSYFR VLARFVTNKY HPDKQFVASL RKAVDRLAAS SQSMYMGSAM
310 320 330 340 350
FQGPFRIDLI LLYSFFRVAD DLVDESQDTE SARMIIEQCD QLLEAKFSHP
360 370 380 390 400
ELFPFSPGYQ EAKHPAPPEL IAAIDSLPVS RLRLEHLKGL IEGFRTDLTF
410 420 430 440 450
SAKPGSFPFV TESDLDTYAY HVASSVAASM LGLVVHHFPD HQFAINVFLR
460 470 480 490 500
RRVVDAGERM GQTLQYINVA RDIARDAAIN RVYLPTTWLK QQGLGPEDVL
510 520 530 540 550
ASPTDSRLEL VRDRLLDRAE FLSASAREEM KFLPDEVQGP FLATVDSYLE
560 570 580
IGAALRRGMR PRTLDDKLRL PLGTRLWVAY RAMAWRK
Length:587
Mass (Da):66,543
Last modified:January 24, 2006 - v1
Checksum:i8BF7D3D45A03AA1D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007167 Genomic DNA. Translation: BAE63386.1.
RefSeqiXP_001824519.1. XM_001824467.1.

Genome annotation databases

EnsemblFungiiCADAORAT00006674; CADAORAP00006543; CADAORAG00006674.
GeneIDi5996605.
KEGGiaor:AOR_1_284084.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007167 Genomic DNA. Translation: BAE63386.1.
RefSeqiXP_001824519.1. XM_001824467.1.

3D structure databases

ProteinModelPortaliQ2U4X9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5062.CADAORAP00006543.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAORAT00006674; CADAORAP00006543; CADAORAG00006674.
GeneIDi5996605.
KEGGiaor:AOR_1_284084.

Phylogenomic databases

eggNOGiNOG47964.
KOiK17841.
OMAiEVFFFII.
OrthoDBiEOG7RBZJ2.

Enzyme and pathway databases

UniPathwayiUPA00799; UER00773.
UPA00802.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR03462. CarR_dom_SF. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequencing and analysis of Aspergillus oryzae."
    Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
    , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
    Nature 438:1157-1161(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 42149 / RIB 40.

Entry informationi

Entry nameiLCPS_ASPOR
AccessioniPrimary (citable) accession number: Q2U4X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: January 24, 2006
Last modified: April 1, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.