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Q2U2I3 (MANF_ASPOR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable mannan endo-1,4-beta-mannosidase F

EC=3.2.1.78
Alternative name(s):
Endo-beta-1,4-mannanase F
Gene names
Name:manF
ORF Names:AO090038000444
OrganismAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) [Complete proteome]
Taxonomic identifier510516 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length463 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans By similarity.

Catalytic activity

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Subcellular location

Secreted By similarity.

Domain

Has a modular structure: a carbohydrate-binding module (CBM) at the N-terminus, a linker rich in serines, and a C-terminal endo-1,4-mannanase catalytic module. The genes for catalytic modules and CBMs seem to have evolved separately and have been linked by gene fusion.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Sequence caution

The sequence BAE64232.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncellulose binding

Inferred from electronic annotation. Source: InterPro

mannan endo-1,4-beta-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 463445Probable mannan endo-1,4-beta-mannosidase F
PRO_0000393716

Regions

Domain19 – 5436CBM1
Region75 – 11844Ser-rich linker
Region119 – 463345Catalyticr

Sites

Active site1961Proton donor By similarity
Active site3051Nucleophile By similarity

Amino acid modifications

Glycosylation871N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q2U2I3 [UniParc].

Last modified April 20, 2010. Version 2.
Checksum: 8926BA2E033A383B

FASTA46349,992
        10         20         30         40         50         60 
MRSLSSIALL SVVGAASAQA GPWAQCGGKS FSGSSECASG WKCQELNEWF SQCVPGAEST 

        70         80         90        100        110        120 
TPTVSSTPTP TDAPSVSITA SVTTGINKSI SVSSASKSTP LPSSSSASPS PRPTGSGSFA 

       130        140        150        160        170        180 
KADGLQFSID GETKYFAGTN AYWLPFQMND ADIDSVFDHL EQAGLKILRV WGFNDVNTAP 

       190        200        210        220        230        240 
SPGTVYFQLH DKEKGTSTIN TGKDGLQRLD YVVAAAEKHG VKLIIPFVNS WDDYGGYNAY 

       250        260        270        280        290        300 
VKAYGGSKTE WFTNEKIQSV YQAYIKAVVS RYRDSPAIFA WELGNEPRCS GCSTDVIHGW 

       310        320        330        340        350        360 
ATKISAYIKS LDPNHMVALG DEGMGLTIGS DQSYPYGTSE GNDFEKNLAI PDIDFGTLHL 

       370        380        390        400        410        420 
YTTDWGIKDN AWGNGWVENH AKACKAAGKP CLFEEYGMKG NHCTDELKWQ KTSLSSGTAA 

       430        440        450        460 
DLIWQYGQQL STGESPKDAY SIFYGTDEWK CAVMDHMENV NKN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP007169 Genomic DNA. Translation: BAE64232.1. Sequence problems.
RefSeqXP_001825365.2. XM_001825313.2.

3D structure databases

ProteinModelPortalQ2U2I3.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5997460.
KEGGaor:AOR_1_742074.

Phylogenomic databases

eggNOGCOG3934.
HOGENOMHOG000169951.
OMASYWVGLM.
OrthoDBEOG7M3J90.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR000254. Cellulose-bd_dom_fun.
IPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00734. CBM_1. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SMARTSM00236. fCBD. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
SSF57180. SSF57180. 1 hit.
PROSITEPS00562. CBM1_1. 1 hit.
PS51164. CBM1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMANF_ASPOR
AccessionPrimary (citable) accession number: Q2U2I3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 20, 2010
Last modified: February 19, 2014
This is version 51 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries