Reviewed, UniProtKB/Swiss-Prot Q2U0C3 (ATG26_ASPOR)

Names and origin

Protein names

Recommended name:
    Sterol 3-beta-glucosyltransferase
    EC=2.4.1.173
Alternative name(s):
    Autophagy-related protein 26

Gene names

Name:	atg26
ORF Names:	AO090011000498

Organism

Aspergillus oryzae [Complete proteome]

Taxonomic identifier

5062 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	1384 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in decane metabolism and autophagy. Involved in the biosynthesis of sterol glucoside By similarity.
Catalytic activity	UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 2 GRAM domains. Contains 1 PH domain.
Sequence caution	The sequence BAE64992.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Autophagy Lipid synthesis Protein transport Steroid biosynthesis Sterol biosynthesis Transport
Cellular component	Cytoplasm Membrane
Domain	Repeat
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sterol 3-beta-glucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1384	1384	Sterol 3-beta-glucosyltransferase		PRO_0000318042
Regions
	Domain	234 – 285	52	GRAM 1
	Domain	285 – 384	100	PH
	Domain	713 – 779	67	GRAM 2

Sequences

	Sequence		Length	Mass (Da)
	Q2U0C3-1 [UniParc]. Last modified February 5, 2008. Version 2. Checksum: A5B4031635513FCB	FASTA	1,384	154,514
	10 20 30 40 50 60 MPNMKPLLED AKRRVDRRLS ASRQSISSSR IFSSAFPDRL KDDHDAQVDY TAPPRGAGSR 70 80 90 100 110 120 DGQLPYMHQS IFSMIAAVGS KSDFHARFDE SSDSEGEAEG QTQKQPGKAS LSNKKKFPLS 130 140 150 160 170 180 PTLKPQSTLE GRGRRHRRSI SDNKLLRSLK PSPKSSKGTE TTVQTEPPTS DEMSPLASPR 190 200 210 220 230 240 RARSATPRAA PILSRMLEAE ALLDKKQSAD QPSSSTKGET DGTSEQSCAS PLSLRLKEMF 250 260 270 280 290 300 GFEMPEKVLM EYACSLLQNI LLQGYMYVTE GHICFYAYLP RKSAVTIRSG YLHKRGRKNP 310 320 330 340 350 360 KYNRYWFSLK GDVLSYYTDP SSLYFPSGHV DLRYGISASL TEQKDKDKEV RDFQVTTDQR 370 380 390 400 410 420 TYYFRADSSA SAKEWVKALQ KVIFRAHNEG DSVKISFPIE SIIDIEESPI TDLAETFKIR 430 440 450 460 470 480 VVESDESYAI DEYYFSFFES GRDAYNFVKG LISEGPMKTS QLLPPPSEQT SPATRARGPR 490 500 510 520 530 540 NRWSLNSDLS QSRGNGIFKT QRKRSASTGQ TNSGPDGIGM SPRQRDLSDS FVNSFEQATD 550 560 570 580 590 600 ASAVLQSMID TTESASQILN RSDVFQSPTI HTLRQRHPSG DRTGRRLSDG TARSTHPNAA 610 620 630 640 650 660 DANRNGQEMQ YASSDSDQGT QHPSKVNSSA PTLNELVKAG AYPLQRAAGF AEYLRSRSRQ 670 680 690 700 710 720 MSNLLASESM GYIEKVSGMW AGGRRHYGET EGVLPDDQDV DPEDKEDGVK HGDRFRAHFA 730 740 750 760 770 780 LPSTERLQAT YYAYLHRVLP LYGKIYISQK KLCFRSLIPG TRTKLILPLK DIENVEKEKG 790 800 810 820 830 840 FRFGYQGLVI IIRGHEELFF EFNTADARDD CAVTVHQSLE SMRFLVESGL LAEQEKDEIE 850 860 870 880 890 900 SAQAEHRMLQ EARLDGAGEH DSHASVNESS ELHPIFDDPR ASIINFKPSE SLRITCLTIG 910 920 930 940 950 960 SRGDVQPYIA LCKGLLAEGH KPKIATHAEF EPWVRQHGID FAPVDGDPAE LMRICVENGM 970 980 990 1000 1010 1020 FTYSFLKEAS TKFRGWIDDL LSSAWASCQD SDLLIESPSA MAGIHIAEAL RIPYFRAFTM 1030 1040 1050 1060 1070 1080 PWSRTRAYPH AFAVPENKMG GAYNYITYVM FDTVFWKAIA GQVNRWRKKQ LGLKATTLDK 1090 1100 1110 1120 1130 1140 MQPNKVPFLY NYSPSVVAPP LDYPDWIRIT GYWFLSEGGN WTPPTDLLDF IHRARSDGKK 1150 1160 1170 1180 1190 1200 IVYIGFGSIV VSDPSALTRT VVESVLKADV RCILSKGWSD RLGDPASAKV EIPLPPEIFQ 1210 1220 1230 1240 1250 1260 IQAAPHDWLF SQIDAAAHHG GAGTTGASLR AGVPTIVKPF FGDQFFFGTR VEDLGVGICM 1270 1280 1290 1300 1310 1320 KKLNVSVFSR ALWEATHSER MIVKARELGA QIRSENGVDT AIQAIYRDLE YAKTLARQRS 1330 1340 1350 1360 1370 1380 IVSSTPFSPT PSAKTTAEQE EDDVDDSEEW TFVGDDTEID VSRRLRDRAV SDADMLPEPV TSAS

References

[1]

"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H. Nature 438:1157-1161(2005) [PubMed: 16372010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP007171 Genomic DNA. Translation: BAE64992.1. Sequence problems.
3D structure databases
SMR	Q2U0C3. Positions 288-383.
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus atg26 in contig AP007171_GR.
Phylogenomic databases
OrthoDB	EOG9908S8.
Enzyme and pathway databases
BRENDA	2.4.1.173. 2240.
Family and domain databases
InterPro	IPR004276. Glyco_trans_28. IPR004182. GRAM. IPR011993. PH_type. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF03033. Glyco_transf_28. 1 hit. PF02893. GRAM. 2 hits. PF00169. PH. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 2 hits. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG26_ASPOR

Accession

Primary (citable) accession number: Q2U0C3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	January 19, 2010
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)