Q2TXB7 (MANBB_ASPOR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-mannosidase B
EC=3.2.1.25

Alternative name(s):
Mannanase B
Short name=Mannase B

Gene names

Name:	mndB
ORF Names:	AO090010000208

Organism

Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) [Complete proteome]

Taxonomic identifier

510516 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus oryzae

Protein attributes

Sequence length	833 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides. Involved in the degradation of mannan and galactomannan By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.
Pathway	Glycan metabolism; N-glycan degradation.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.
Sequence caution	The sequence BAE66106.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?		Potential
	Chain	? – 833		Probable beta-mannosidase B		PRO_0000394656
Sites
	Active site	432	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	256	1	N-linked (GlcNAc...) Potential
	Glycosylation	712	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2TXB7 [UniParc]. Last modified June 15, 2010. Version 2. Checksum: 716AB5C9B57FA0D7	FASTA	833	94,718
	10 20 30 40 50 60 MAAFSQYPLS TGWSFKDSDD QSPEAWMPVP VVPSVAHQDL QANQKLKNPY IGFNELDARW 70 80 90 100 110 120 VNDKSWTYRT VFQKPAVAAG SSIILAFDGL DTFATVKLDG SVILQSDNMF LAHRVDVTKA 130 140 150 160 170 180 LEAEGDHVLE IDFDCAMRRA RELREKDTKH NWASFNGDPA RMAVRKAQYH WGWDWGPLLS 190 200 210 220 230 240 TAGIWREVRL EVYSAKISDL WTEVELASDH QTARVSAFAE VDAADSVDSY KASFLLSLHG 250 260 270 280 290 300 KEVAREVATL KDKVANVTFD VTQPSLWWPN GYGDPALYEI SVSLEKEDCE IHSVSKKIGI 310 320 330 340 350 360 RTAELIQQPD RHGKSFFFRI NGVDVFCGGS CWIPADNLLP SITAERYRKW IELMVAGRQV 370 380 390 400 410 420 MIRVWGGGCY EDDSFYQACD ELGVLVWQDF MFGCGNYPTW PELLESIEKE ANYNVRRLRH 430 440 450 460 470 480 HPSIVVYVGN NEDYQVQESA GLVYDYEDKN PENWLKTDFP ARYIYEKLLP SVVEKLSPKT 490 500 510 520 530 540 VYHPGSPWGD GKITSDPTVG DMHQWNVWHG TQEKYQIFDT LGGRFNSEFG MEAFPHMSTI 550 560 570 580 590 600 EYFVENEADK YPQSHVLDFH NKADGHERRI ATYLTYVYLT QVVQAETMMF GYRGWRRQWG 610 620 630 640 650 660 DERHCGGALL WQLNDCWPTI SWAIVDYFLR PKPAFYAVAR VLKPIAVGVR REHHDWSVTH 670 680 690 700 710 720 AQPPKTSKYE LWIASSLQKE TVGTIELRFL SVNTGLDVRA PILRDNVKIV PNGTTNILEG 730 740 750 760 770 780 VIDHKAQPEP HVLAARLWVD GEVTARDVDW PQPFKYLDLS DRGLEVNKVS ESGNEQKLLI 790 800 810 820 830 TAKKPVKCLV FEERDGIRVS DSAMDIVPGD GQTVTVTGLK AGDAPLKYKY LGQ

References

[1]

"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H. Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP007175 Genomic DNA. Translation: BAE66106.1. Sequence problems.
3D structure databases
ProteinModelPortal	Q2TXB7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAORAT00004309; CADAORAP00004234; CADAORAG00004309.
Phylogenomic databases
eggNOG	COG3250.
HOGENOM	HOG000186861.
OrthoDB	EOG408RH8.
Enzyme and pathway databases
UniPathway	UPA00280.
Family and domain databases
Gene3D	2.60.120.260. 1 hit. 2.60.40.320. 1 hit. 3.20.20.80. 1 hit.
InterPro	IPR008979. Galactose-bd-like. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006102. Glyco_hydro_2_Ig-like. IPR006104. Glyco_hydro_2_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00703. Glyco_hydro_2. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF49303. Glyco_hydro_2Ig. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. False negative. PS00608. GLYCOSYL_HYDROL_F2_2. False negative. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL4155.

Entry information

Entry name

MANBB_ASPOR

Accession

Primary (citable) accession number: Q2TXB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	June 15, 2010
Last modified:	May 29, 2013
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program