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Protein

Endo-beta-1,4-glucanase celB

Gene

celB

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

pH dependencei

Optimum pH is 4.0. Stable between pH 3.0 and 7.0.1 Publication

Temperature dependencei

Optimum temperature is 45 degrees Celsius. Stable up to 50 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei214NucleophileBy similarity1
Active sitei219Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellulose catabolic process Source: UniProtKB-KW
  • glucan catabolic process Source: AspGD

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH7 Glycoside Hydrolase Family 7

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-beta-1,4-glucanase celB (EC:3.2.1.4)
Short name:
Endoglucanase celB
Alternative name(s):
Carboxymethylcellulase celB
Cellulase B
Gene namesi
Name:celB
ORF Names:AO090010000314
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006564 Componenti: Chromosome 8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000039515718 – 416Endo-beta-1,4-glucanase celBAdd BLAST399

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi45N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi104N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi117N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi135N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi233N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi278N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi292N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi382N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi5062.CADAORAP00004325

Structurei

3D structure databases

ProteinModelPortaliQ2TX26
SMRiQ2TX26
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000182210
KOiK19357
OMAiQERCIAD
OrthoDBiEOG092C29WB

Family and domain databases

CDDicd07999 GH7_CBH_EG, 1 hit
Gene3Di2.70.100.10, 1 hit
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR001722 Glyco_hydro_7
IPR037019 Glyco_hydro_7_sf
PANTHERiPTHR33753 PTHR33753, 1 hit
PfamiView protein in Pfam
PF00840 Glyco_hydro_7, 1 hit
PRINTSiPR00734 GLHYDRLASE7
SUPFAMiSSF49899 SSF49899, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2TX26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIWTLAPFVA LLPLVTAQQV GTTADAHPRL TTYKCTSQNG CTRQNTSVVL
60 70 80 90 100
DAATHFIHKK GTQTSCTNSN GLDTAICPDK QTCADNCVVD GITDYASYGV
110 120 130 140 150
QTKNDTLTLQ QYLQTGNATK SLSPRVYLLA EDGENYSMLK LLNQEFTFDV
160 170 180 190 200
DASTLVCGMN GALYLSEMEA SGGKSSLNQA GAKYGTGYCD AQCYTTPWIN
210 220 230 240 250
GEGNTESVGS CCQEMDIWEA NARATGLTPH PCNTTGLYEC SGSGCGDSGV
260 270 280 290 300
CDKAGCGFNP YGLGAKDYYG YGLKVNTNET FTVVTQFLTN DNTTSGQLSE
310 320 330 340 350
IRRLYIQNGQ VIQNAAVTSG GKTVDSITKD FCSGEGSAFN RLGGLEEMGH
360 370 380 390 400
ALGRGMVLAL SIWNDAGSFM QWLDGGSAGP CNATEGNPAL IEKLYPDTHV
410
KFSKIRWGDI GSTYRH
Length:416
Mass (Da):44,448
Last modified:January 24, 2006 - v1
Checksum:i437ADE03BF84AB53
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48V → L in BAA22589 (PubMed:9008887).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83732 Genomic DNA Translation: BAA22589.1
AP007175 Genomic DNA Translation: BAE66197.1
RefSeqiXP_001827330.1, XM_001827278.1

Genome annotation databases

EnsemblFungiiBAE66197; BAE66197; AO090010000314
GeneIDi5999464
KEGGiaor:AO090010000314

Similar proteinsi

Entry informationi

Entry nameiCELB_ASPOR
AccessioniPrimary (citable) accession number: Q2TX26
Secondary accession number(s): O13455
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: January 24, 2006
Last modified: March 28, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health